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Biosensors 2018, 8(4), 87; https://doi.org/10.3390/bios8040087

Functionalized Gold Nanoparticles as Biosensors for Monitoring Cellular Uptake and Localization in Normal and Tumor Prostatic Cells

1
Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, 80078 Pozzuoli, Italy
2
Institute of Agro-environmental and Forest Biology, National Research Council of Italy, 80131 Naples, Italy
*
Authors to whom correspondence should be addressed.
Received: 18 July 2018 / Revised: 27 September 2018 / Accepted: 29 September 2018 / Published: 4 October 2018
(This article belongs to the Special Issue Applications of Raman Techniques in Biosensing)
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Abstract

In the present contribution the fabrication and characterization of functionalized gold nanospheres of uniform shape and controlled size is reported. These nano-objects are intended to be used as Surface Enhanced Raman Spectroscopy (SERS) sensors for in-vitro cellular uptake and localization. Thiophenol was used as molecular reporter and was bound to the Au surface by a chemisorption process in aqueous solution. The obtained colloidal solution was highly stable and no aggregation of the single nanospheres into larger clusters was observed. The nanoparticles were incubated in human prostatic cells with the aim of developing a robust, SERS-based method to differentiate normal and tumor cell lines. SERS imaging experiments showed that tumor cells uptake considerably larger amounts of nanoparticles in comparison to normal cells (up to 950% more); significant differences were also observed in the uptake kinetics. This largely different behaviour might be exploited in diagnostic and therapeutic applications. View Full-Text
Keywords: SERS; nanomaterials; biosensing; prostate cancer; single cell spectroscopy SERS; nanomaterials; biosensing; prostate cancer; single cell spectroscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Pannico, M.; Calarco, A.; Peluso, G.; Musto, P. Functionalized Gold Nanoparticles as Biosensors for Monitoring Cellular Uptake and Localization in Normal and Tumor Prostatic Cells. Biosensors 2018, 8, 87.

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