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Article

A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

by 1,2,3, 1,2,3, 1,2,3, 1,2,3 and 1,2,3,*
1
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China
3
Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai 201306, China
*
Author to whom correspondence should be addressed.
Biosensors 2022, 12(7), 456; https://doi.org/10.3390/bios12070456
Received: 29 May 2022 / Revised: 20 June 2022 / Accepted: 24 June 2022 / Published: 26 June 2022
(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)
In this research, we designed a rapid tricolour immunochromatographic test strip with double test lines (TS-DTL) and two-colour AuNP probes, which realised the simultaneous detection of tricaine mesylate (TMS) and malachite green (MG). Through a distinct tricolour system (red T1 line, blue T2 line and purple C line), a visual identification of TMS (0.2 μg/mL) and MG (0.5 μg/mL) was quickly achieved on site, which improved the accuracy of naked eye observations. The LODs of TMS in aquaculture water, fish and shrimp were 11.0, 29.6 and 61.4 ng/mL, respectively. MG LODs were 47.0 ng/mL (aquaculture water), 82.8 ng/mL (fish) and 152.4 ng/mL (shrimp). The LOD of MG was close to the similar TS methods. However, visual detection of TMS could meet the requirements of the residue limit (1 μg/mL) of TMS in the USA, and the quantitative detection of TMS was over 16 times lower than the USA standard. The developed platform was rapid (~20 min, HPLC~3 h) and accurate, which was verified using a traditional HPLC method. The recovery rates ranged from 82.2% to 108.6% in three types of real samples, indicating a potential application in on-site fast screening or multiple detection for TMS and MG residues in aquatic products. View Full-Text
Keywords: tricolour test strip; tricaine mesylate; malachite green; simultaneous detection; Au nanoparticles tricolour test strip; tricaine mesylate; malachite green; simultaneous detection; Au nanoparticles
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MDPI and ACS Style

Lu, Y.-K.; Xu, D.; Liu, W.-Y.; Xie, J.; Lu, Y. A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green. Biosensors 2022, 12, 456. https://doi.org/10.3390/bios12070456

AMA Style

Lu Y-K, Xu D, Liu W-Y, Xie J, Lu Y. A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green. Biosensors. 2022; 12(7):456. https://doi.org/10.3390/bios12070456

Chicago/Turabian Style

Lu, Yi-Kuan, Di Xu, Wen-Yue Liu, Jing Xie, and Ying Lu. 2022. "A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green" Biosensors 12, no. 7: 456. https://doi.org/10.3390/bios12070456

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