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Article

Enhancing PD-1 Gene Silence in T Lymphocytes by Comparing the Delivery Performance of Two Inorganic Nanoparticle Platforms

by 1, 1,*, 1, 2 and 1,*
1
Australian Institute for Bioengineering and Nanotechnology, the University of Queensland, St. Lucia 4072, QLD, Australia
2
School of Biomedical Sciences, the University of Queensland, St. Lucia 4072, QLD, Australia
*
Authors to whom correspondence should be addressed.
Nanomaterials 2019, 9(2), 159; https://doi.org/10.3390/nano9020159
Received: 18 December 2018 / Revised: 18 January 2019 / Accepted: 24 January 2019 / Published: 28 January 2019
Suitable carriers are crucial to RNAi applications for cancer genotherapy and T-cell immunotherapy. In this research, we selected two extensively-investigated biocompatible inorganic nanoparticle carriers, i.e., layered double hydroxide (LDH) and lipid-coated calcium phosphate (LCP) and then compared their efficacy for siRNA delivery in T cells, in order to understand which carrier is more efficient in delivering functional programmed cell death protein 1 siRNA (PD-1 siRNA) to suspended T lymphocytes. Both LDH and LCP nanoparticles quickly delivered gene segment to mouse T cell lines (EL4), while the LCP nanoparticles exhibited more cellular uptake and higher PD-1 gene silence efficiency. We further demonstrated that LCP nanoparticles successfully reduced the expression of PD-1 in human ex vivo tumor infiltrating lymphocytes (TILs). Thus, LCP nanoparticles can be used as a better nano-carrier for gene therapy in lymphocytes, especially in regards to TIL-related cancer immunotherapy. View Full-Text
Keywords: layered double hydroxide (LDH) nanoparticle; lipid-coated calcium phosphate (LCP) nanoparticle; programmed cell death protien-1 (PD-1); human tumor infiltrating lymphocytes (TILs); PD-1 gene silencing; EL4 cells layered double hydroxide (LDH) nanoparticle; lipid-coated calcium phosphate (LCP) nanoparticle; programmed cell death protien-1 (PD-1); human tumor infiltrating lymphocytes (TILs); PD-1 gene silencing; EL4 cells
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MDPI and ACS Style

Wu, Y.; Gu, W.; Li, L.; Chen, C.; Xu, Z.P. Enhancing PD-1 Gene Silence in T Lymphocytes by Comparing the Delivery Performance of Two Inorganic Nanoparticle Platforms. Nanomaterials 2019, 9, 159. https://doi.org/10.3390/nano9020159

AMA Style

Wu Y, Gu W, Li L, Chen C, Xu ZP. Enhancing PD-1 Gene Silence in T Lymphocytes by Comparing the Delivery Performance of Two Inorganic Nanoparticle Platforms. Nanomaterials. 2019; 9(2):159. https://doi.org/10.3390/nano9020159

Chicago/Turabian Style

Wu, Yanheng; Gu, Wenyi; Li, Li; Chen, Chen; Xu, Zhi P. 2019. "Enhancing PD-1 Gene Silence in T Lymphocytes by Comparing the Delivery Performance of Two Inorganic Nanoparticle Platforms" Nanomaterials 9, no. 2: 159. https://doi.org/10.3390/nano9020159

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