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Open AccessArticle

Development and Characterization of Solid Lipid Nanoparticles Loaded with a Highly Active Doxorubicin Derivative

1
Dipartimento di Scienza e Tecnologia del Farmaco, University of Turin, Via P. Giuria 9, 10125 Turin, Italy
2
Dipartimento di Scienze della Salute, University of Eastern Piedmont Amedeo Avogadro, Via P. Solaroli 15, 28100 Novara, Italy
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this manuscript.
Nanomaterials 2018, 8(2), 110; https://doi.org/10.3390/nano8020110
Received: 10 January 2018 / Revised: 9 February 2018 / Accepted: 13 February 2018 / Published: 16 February 2018
(This article belongs to the Special Issue Pharmaceutical Nanotechnology)
Solid lipid nanoparticles (SLNs) comprise a versatile drug delivery system that has been developed for the treatment of a variety of diseases. The present study will investigate the feasibility of entrapping an active doxorubicin prodrug (a squalenoyl-derivative) in SLNs. The doxorubicin derivative-loaded SLNs are spherically shaped, have a mean diameter of 300–400 nm and show 85% w/w drug entrapment efficiency. The effects on cell growth of loaded SLNs, free doxorubicin and the prodrug have been examined using cytotoxicity and colony-forming assays in both human ovarian cancer line A2780 wild-type and doxorubicin-resistant cells. Further assessments as to the treatment’s ability to induce cell death by apoptosis have been carried out by analyzing annexin-V staining and the activation of caspase-3. The in vitro data demonstrate that the delivery of the squalenoyl-doxorubicin derivative by SLNs increases its cytotoxic activity, as well as its apoptosis effect. This effect was particularly evident in doxorubicin-resistant cells. View Full-Text
Keywords: solid lipid nanoparticles; doxorubicin; anticancer agent; nanoassemblies; anticancer prodrugs solid lipid nanoparticles; doxorubicin; anticancer agent; nanoassemblies; anticancer prodrugs
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MDPI and ACS Style

Stella, B.; Peira, E.; Dianzani, C.; Gallarate, M.; Battaglia, L.; Gigliotti, C.L.; Boggio, E.; Dianzani, U.; Dosio, F. Development and Characterization of Solid Lipid Nanoparticles Loaded with a Highly Active Doxorubicin Derivative. Nanomaterials 2018, 8, 110. https://doi.org/10.3390/nano8020110

AMA Style

Stella B, Peira E, Dianzani C, Gallarate M, Battaglia L, Gigliotti CL, Boggio E, Dianzani U, Dosio F. Development and Characterization of Solid Lipid Nanoparticles Loaded with a Highly Active Doxorubicin Derivative. Nanomaterials. 2018; 8(2):110. https://doi.org/10.3390/nano8020110

Chicago/Turabian Style

Stella, Barbara; Peira, Elena; Dianzani, Chiara; Gallarate, Marina; Battaglia, Luigi; Gigliotti, Casimiro L.; Boggio, Elena; Dianzani, Umberto; Dosio, Franco. 2018. "Development and Characterization of Solid Lipid Nanoparticles Loaded with a Highly Active Doxorubicin Derivative" Nanomaterials 8, no. 2: 110. https://doi.org/10.3390/nano8020110

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