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Article

Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes

1
Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland
2
Cell Biology, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland
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Electron Microscopy Unit, Helsinki Institute of Life Science—Institute of Biotechnology, University of Helsinki, FI-00014 Helsinki, Finland
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Institute of Biomedicine, Faculty of Medicine, University of Turku, 20520 Turku, Finland
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Cancer Research Laboratory FICAN West, Institute of Biomedicine, University of Turku, 20520 Turku, Finland
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Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland
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CryoCapCell, 155 Boulevard de l’Hopital, 75013 Paris, France
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Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan
*
Author to whom correspondence should be addressed.
Nanomaterials 2021, 11(1), 14; https://doi.org/10.3390/nano11010014
Received: 18 November 2020 / Revised: 17 December 2020 / Accepted: 21 December 2020 / Published: 23 December 2020
(This article belongs to the Special Issue Application of Nanomaterials in Biomedical Imaging and Cancer Therapy)
Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets. View Full-Text
Keywords: correlative microscopy; 3D CLEM; volume imaging correlative microscopy; 3D CLEM; volume imaging
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MDPI and ACS Style

Prabhakar, N.; Belevich, I.; Peurla, M.; Heiligenstein, X.; Chang, H.-C.; Sahlgren, C.; Jokitalo, E.; Rosenholm, J.M. Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes. Nanomaterials 2021, 11, 14. https://doi.org/10.3390/nano11010014

AMA Style

Prabhakar N, Belevich I, Peurla M, Heiligenstein X, Chang H-C, Sahlgren C, Jokitalo E, Rosenholm JM. Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes. Nanomaterials. 2021; 11(1):14. https://doi.org/10.3390/nano11010014

Chicago/Turabian Style

Prabhakar, Neeraj, Ilya Belevich, Markus Peurla, Xavier Heiligenstein, Huan-Cheng Chang, Cecilia Sahlgren, Eija Jokitalo, and Jessica M. Rosenholm 2021. "Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes" Nanomaterials 11, no. 1: 14. https://doi.org/10.3390/nano11010014

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