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Open AccessArticle

Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic Tubes

1
Tokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, Japan
2
Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
3
Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan
4
Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
*
Author to whom correspondence should be addressed.
J. Funct. Biomater. 2019, 10(3), 43; https://doi.org/10.3390/jfb10030043
Received: 12 August 2019 / Revised: 13 September 2019 / Accepted: 15 September 2019 / Published: 17 September 2019
Compared with platelet-rich plasma, the preparation of platelet-rich fibrin (PRF) is simple and has not been overly modified. However, it was recently demonstrated that centrifugation conditions influence the composition of PRF and that silica microparticles from silica-coated plastic tubes can enter the PRF matrix. These factors may also modify platelet distribution. To examine these possibilities, we prepared PRF matrices using various types of blood-collection tubes (plain glass tubes and silica-containing plastic tubes) and different centrifugation speeds. The protocols of concentrated growth factors and advanced-PRF represented high- and low-speed centrifugation, respectively. Platelet distribution in the PRF matrix was examined immunohistochemically. Using low-speed centrifugation, platelets were distributed homogeneously within the PRF matrix regardless of tube types. In high-speed centrifugation, platelets were distributed mainly on one surface region of the PRF matrix in glass tubes, whereas in silica-coated tubes, platelet distribution was commonly more diffusive than in glass tubes. Therefore, both blood-collection tube types and centrifugal conditions appeared to influence platelet distribution in the PRF matrix. Platelets distributed in the deep regions of the PRF matrix may contribute to better growth factor retention and release. However, clinicians should be careful in using silica-coated tubes because their silica microparticles may be a health hazard. View Full-Text
Keywords: advanced platelet-rich fibrin; concentrated growth factors; platelets; CD41; centrifugal force; silica advanced platelet-rich fibrin; concentrated growth factors; platelets; CD41; centrifugal force; silica
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Tsujino, T.; Masuki, H.; Nakamura, M.; Isobe, K.; Kawabata, H.; Aizawa, H.; Watanabe, T.; Kitamura, Y.; Okudera, H.; Okuda, K.; Nakata, K.; Kawase, T. Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic Tubes. J. Funct. Biomater. 2019, 10, 43.

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