Text Correction
Authors would like to correct an error in the GC conditions for VFA analysis [1]. Higher temperature was used for the detector than previously reported. We also omitted the individuals that analyzed our samples. Section 2.8. Volatile Fatty Acid has been changed as following:
Rumen fluid samples that were preserved with 25% metaphosphoric–crotonic acid were thawed and centrifuged at 10,000× g for 15 min at 4 °C using a Thermo Fisher Scientific centrifuge (model Sorvall X4R Pro-MD; Thermo Electron LED GmbH, Osterode, Germany) and analyzed according to the protocol in [42] for acetate, propionate, butyrate, isobutyrate, isovalerate, and valerate concentrations using an automated GC (model 7890 B; Agilent Technologies, Santa Clara, CA, USA) and a flame ionization detector. Volatile fatty acids were separated on a capillary column ZB FFAP (30 m × 0.25 mm i.d., 0.25 um; Phenomenex Inc., Torrance, CA, USA) using a metaphosphoric–crotonic acid mixture as an internal standard. The split ratio of 1:12 in the injector port was at a temperature of 250 °C with a flow rate of 1 mL/min of helium. The oven temperature was initially set at 120 °C for 0.8 min; it then increased by 8 degrees per minute until it reached 140 °C. The detector temperature was maintained at 280 °C.
Reference
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