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Open AccessArticle

Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity

1
Institute for Biomedical Research “Alberto Sols” (IIBM), Spanish National Research Council-Autonomous University of Madrid (CSIC-UAM), 28029 Madrid, Spain
2
Centre for Biomedical Network Research on Rare Diseases (CIBERER, CIBER), Institute of Health Carlos III (ISCIII), 28029 Madrid, Spain
3
Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, 48080 Leioa, Spain
4
Hospital La Paz Institute for Health Research (IdiPAZ), 28029 Madrid, Spain
5
Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, 50134 Firenze, Italy
6
Instituto Biofisika (CSIC, UPV/EHU), Barrio Sarriena S/N 48940, 48080 Leioa, Spain
*
Authors to whom correspondence should be addressed.
Equal senior contribution.
J. Clin. Med. 2019, 8(9), 1464; https://doi.org/10.3390/jcm8091464
Received: 24 July 2019 / Revised: 23 August 2019 / Accepted: 12 September 2019 / Published: 14 September 2019
(This article belongs to the Special Issue Therapies for Hearing Loss)
Cisplatin is a chemotherapeutic agent that causes the irreversible death of auditory sensory cells, leading to hearing loss. Local administration of cytoprotective drugs is a potentially better option co-therapy for cisplatin, but there are strong limitations due to the difficulty of accessing the inner ear. The use of nanocarriers for the efficient delivery of drugs to auditory cells is a novel approach for this problem. Solid lipid nanoparticles (SLNs) are biodegradable and biocompatible nanocarriers with low solubility in aqueous media. We show here that stearic acid-based SLNs have the adequate particle size, polydispersity index and ζ-potential, to be considered optimal nanocarriers for drug delivery. Stearic acid-based SLNs were loaded with the fluorescent probe rhodamine to show that they are efficiently incorporated by auditory HEI-OC1 (House Ear Institute-Organ of Corti 1) cells. SLNs were not ototoxic over a wide dose range. Glucocorticoids are used to decrease cisplatin-induced ototoxicity. Therefore, to test SLNs’ drug delivery efficiency, dexamethasone and hydrocortisone were tested either alone or loaded into SLNs and tested in a cisplatin-induced ototoxicity in vitro assay. Our results indicate that the encapsulation in SLNs increases the protective effect of low doses of hydrocortisone and lengthens the survival of HEI-OC1 cells treated with cisplatin. View Full-Text
Keywords: nanocarriers; dexamethasone; hydrocortisone; otic protection; stearic acid-based solid lipid nanoparticle (SLN) nanocarriers; dexamethasone; hydrocortisone; otic protection; stearic acid-based solid lipid nanoparticle (SLN)
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MDPI and ACS Style

Cervantes, B.; Arana, L.; Murillo-Cuesta, S.; Bruno, M.; Alkorta, I.; Varela-Nieto, I. Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity. J. Clin. Med. 2019, 8, 1464.

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