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Open AccessArticle

Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes

1
Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, Japan
2
Toray Research Center, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan
*
Author to whom correspondence should be addressed.
Membranes 2014, 4(3), 491-508; https://doi.org/10.3390/membranes4030491
Received: 26 February 2014 / Revised: 22 July 2014 / Accepted: 25 July 2014 / Published: 8 August 2014
We isolated a high-purity carp glycophorin from carp erythrocyte membranes following extraction using the lithium diiodosalicylate (LIS)-phenol method and streptomycin treatment. The main carp glycophorin was observed to locate at the position of the carp and human band-3 proteins on an SDS-polyacrylamide gel. Only the N-glycolylneuraminic acid (NeuGc) form of sialic acid was detected in the carp glycophorin. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. We observed bacteriostatic activity against five strains of bacteria, including two known fish pathogens. Fractions from the carp erythrocyte membrane, the glycophorin oligosaccharide and the P-1 also exhibited bacteriostatic activity; whereas the glycolipid fraction and the glycophorin fraction without sialic acid did not show the activity. The carp glycophorin molecules attach to the flagellum of V. anguillarum or the cell surface of M. luteus and inhibited bacterial growth. View Full-Text
Keywords: fish; red blood cell membranes; erythrocyte; glycophorin; oligosaccharide; sialic acid; N-glycolylneuraminic acid; bacteriostatic activity fish; red blood cell membranes; erythrocyte; glycophorin; oligosaccharide; sialic acid; N-glycolylneuraminic acid; bacteriostatic activity
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Aoki, T.; Chimura, K.; Nakao, N.; Mizuno, Y. Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes. Membranes 2014, 4, 491-508.

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