Next Article in Journal
Schistosoma haematobium Extracellular Vesicle Proteins Confer Protection in a Heterologous Model of Schistosomiasis
Previous Article in Journal
Proteomic Analysis of Human Immune Responses to Live-Attenuated Tularemia Vaccine
Article

Alterations in the Human Plasma Lipidome in Response to Tularemia Vaccination

1
Department of Biochemistry, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA
2
The Emmes Company, Rockville, MD 20850, USA
3
Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, MO 63104, USA
4
Division of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USA
5
Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
6
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Decatur, GA 30030, USA
7
Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
8
Biomedical Advanced Research and Development Authority, US Department of Health and Human Services, Washington, DC 20201, USA
9
Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20892, USA
10
Department of Pediatrics, Emory University School of Medicine and Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA
11
Department of Internal Medicine, Saint Louis University School of Medicine, St. Louis, MO 63104, USA
12
Division of Infectious Diseases and Immunology, Department of Medicine, and New York University (NYU) Langone Vaccine Center, NYU School of Medicine, New York, NY 10016, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Vaccines 2020, 8(3), 414; https://doi.org/10.3390/vaccines8030414
Received: 11 March 2020 / Revised: 14 April 2020 / Accepted: 24 April 2020 / Published: 24 July 2020
(This article belongs to the Section Attenuated/Inactivated/Live and Vectored Vaccines)
Tularemia is a highly infectious and contagious disease caused by the bacterium Francisella tularensis. To better understand human response to a live-attenuated tularemia vaccine and the biological pathways altered post-vaccination, healthy adults were vaccinated, and plasma was collected pre- and post-vaccination for longitudinal lipidomics studies. Using tandem mass spectrometry, we fully characterized individual lipid species within predominant lipid classes to identify changes in the plasma lipidome during the vaccine response. Separately, we targeted oxylipins, a subset of lipid mediators involved in inflammatory pathways. We identified 14 differentially abundant lipid species from eight lipid classes. These included 5-hydroxyeicosatetraenoic acid (5-HETE) which is indicative of lipoxygenase activity and, subsequently, inflammation. Results suggest that 5-HETE was metabolized to a dihydroxyeicosatrienoic acid (DHET) by day 7 post-vaccination, shedding light on the kinetics of the 5-HETE-mediated inflammatory response. In addition to 5-HETE and DHET, we observed pronounced changes in 34:1 phosphatidylinositol, anandamide, oleamide, ceramides, 16:1 cholesteryl ester, and other glycerophospholipids; several of these changes in abundance were correlated with serum cytokines and T cell activation. These data provide new insights into alterations in plasma lipidome post-tularemia vaccination, potentially identifying key mediators and pathways involved in vaccine response and efficacy. View Full-Text
Keywords: lipidomics; oxylipins; tularemia; 5-hydroxyeicosatetraenoic (5HETE); inflammation; vaccine response lipidomics; oxylipins; tularemia; 5-hydroxyeicosatetraenoic (5HETE); inflammation; vaccine response
Show Figures

Graphical abstract

MDPI and ACS Style

Maner-Smith, K.M.; Goll, J.B.; Khadka, M.; Jensen, T.L.; Colucci, J.K.; Gelber, C.E.; Albert, C.J.; Bosinger, S.E.; Franke, J.D.; Natrajan, M.; Rouphael, N.; Johnson, R.A.; Sanz, P.; Anderson, E.J.; Hoft, D.F.; Mulligan, M.J.; Ford, D.A.; Ortlund, E.A. Alterations in the Human Plasma Lipidome in Response to Tularemia Vaccination. Vaccines 2020, 8, 414. https://doi.org/10.3390/vaccines8030414

AMA Style

Maner-Smith KM, Goll JB, Khadka M, Jensen TL, Colucci JK, Gelber CE, Albert CJ, Bosinger SE, Franke JD, Natrajan M, Rouphael N, Johnson RA, Sanz P, Anderson EJ, Hoft DF, Mulligan MJ, Ford DA, Ortlund EA. Alterations in the Human Plasma Lipidome in Response to Tularemia Vaccination. Vaccines. 2020; 8(3):414. https://doi.org/10.3390/vaccines8030414

Chicago/Turabian Style

Maner-Smith, Kristal M., Johannes B. Goll, Manoj Khadka, Travis L. Jensen, Jennifer K. Colucci, Casey E. Gelber, Carolyn J. Albert, Steven E. Bosinger, Jacob D. Franke, Muktha Natrajan, Nadine Rouphael, Robert A. Johnson, Patrick Sanz, Evan J. Anderson, Daniel F. Hoft, Mark J. Mulligan, David A. Ford, and Eric A. Ortlund 2020. "Alterations in the Human Plasma Lipidome in Response to Tularemia Vaccination" Vaccines 8, no. 3: 414. https://doi.org/10.3390/vaccines8030414

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop