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Article

Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis

1
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
2
Interdepartmental Graduate Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX 77030, USA
3
Graduate Program in Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
4
Department of Chemistry, Rice University, Houston, TX 77005, USA
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Department of Medicine, Division of Rheumatology, Icahn School of Medicine at Mount Sinai, New York, NY 11030, USA
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The NanoCarbon Center, Rice University, Houston, TX 77005, USA
7
Center for Drug Discovery and Biology of Inflammation Center, Baylor College of Medicine, Houston, TX 77030, USA
*
Authors to whom correspondence should be addressed.
M.R.T. and R.H. contributed equally to this work.
Antioxidants 2020, 9(10), 1005; https://doi.org/10.3390/antiox9101005
Received: 4 August 2020 / Revised: 13 October 2020 / Accepted: 14 October 2020 / Published: 16 October 2020
(This article belongs to the Special Issue Nanoantioxidants)
Reactive oxygen species have been involved in the pathogenesis of rheumatoid arthritis (RA). Our goal was to determine the effects of selectively scavenging superoxide (O2•−) and hydroxyl radicals with antioxidant nanoparticles, called poly(ethylene glycol)-functionalized hydrophilic carbon clusters (PEG-HCCs), on the pathogenic functions of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA) and on the progression of an animal model of RA. We used human FLS from patients with RA to determine PEG-HCC internalization and effects on FLS cytotoxicity, invasiveness, proliferation, and production of proteases. We used the pristane-induced arthritis (PIA) rat model of RA to assess the benefits of PEG-HCCs on reducing disease severity. PEG-HCCs were internalized by RA-FLS, reduced their intracellular O2•−, and reduced multiple measures of their pathogenicity in vitro, including proliferation and invasion. In PIA, PEG-HCCs caused a 65% reduction in disease severity, as measured by a standardized scoring system of paw inflammation and caused a significant reduction in bone and tissue damage, and circulating rheumatoid factor. PEG-HCCs did not induce lymphopenia during PIA. Our study demonstrated a role for O2•− and hydroxyl radicals in the pathogenesis of a rat model of RA and showed efficacy of PEG-HCCs in treating a rat model of RA. View Full-Text
Keywords: synovial fibroblast; oxidative stress; nanomaterials synovial fibroblast; oxidative stress; nanomaterials
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MDPI and ACS Style

Tanner, M.R.; Huq, R.; Sikkema, W.K.A.; Nilewski, L.G.; Yosef, N.; Schmitt, C.; Flores-Suarez, C.P.; Raugh, A.; Laragione, T.; Gulko, P.S.; Tour, J.M.; Beeton, C. Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis. Antioxidants 2020, 9, 1005. https://doi.org/10.3390/antiox9101005

AMA Style

Tanner MR, Huq R, Sikkema WKA, Nilewski LG, Yosef N, Schmitt C, Flores-Suarez CP, Raugh A, Laragione T, Gulko PS, Tour JM, Beeton C. Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis. Antioxidants. 2020; 9(10):1005. https://doi.org/10.3390/antiox9101005

Chicago/Turabian Style

Tanner, Mark R., Redwan Huq, William K. A. Sikkema, Lizanne G. Nilewski, Nejla Yosef, Cody Schmitt, Carlos P. Flores-Suarez, Arielle Raugh, Teresina Laragione, Pércio S. Gulko, James M. Tour, and Christine Beeton. 2020. "Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis" Antioxidants 9, no. 10: 1005. https://doi.org/10.3390/antiox9101005

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