Next Article in Journal
Protective Effect of Aqueous Extract from the Leaves of Justicia tranquebariesis against Thioacetamide-Induced Oxidative Stress and Hepatic Fibrosis in Rats
Previous Article in Journal
The Effects of Beta-Hydroxy-Beta-Methylbutyrate-Free Acid Supplementation and Resistance Training on Oxidative Stress Markers: A Randomized, Double-Blind, Placebo-Controlled Study
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle

The Extract of D. dasycarpus Ameliorates Oxazolone-Induced Skin Damage in Mice by Anti-Inflammatory and Antioxidant Mechanisms

1
Department of Applied Cosmetology and Master Program of Cosmetic Sciences, Hungkuang University, Taichung 43302, Taiwan
2
Department of Medical Laboratory Science and Biotechnology, College of Medicine, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan
3
Niuer International Skincare Science Research Institute, 9F, No. 266, Ruiguang Rd., Neihu Dist., Taipei 11491, Taiwan
*
Author to whom correspondence should be addressed.
Antioxidants 2018, 7(6), 77; https://doi.org/10.3390/antiox7060077
Received: 1 June 2018 / Revised: 12 June 2018 / Accepted: 12 June 2018 / Published: 15 June 2018
  |  
PDF [2666 KB, uploaded 15 June 2018]
  |  

Abstract

Dictamni dasycarpus is a type of Chinese medicine made from the root bark of D. dasycarpus. It has been reported to show a wide spectrum of biological and pharmacological effects, for example, it has been used widely for the treatment of rheumatism, nettle rash, itching, jaundice, chronic hepatitis and skin diseases. In the current study, D. dasycarpus extract was investigated for its antioxidant and anti-inflammatory effects, as well as its capability to alleviate oxazolone-induced skin damage in mice. The possible anti-inflammatory mechanism of D. dasycarpus extract against oxidative challenge was elucidated by measuring the levels of reactive oxygen species (ROS) production, interleukin-6, Tumor necrosis factor-α, NLRP3 (NACHT, LRR and PYD domains-containing protein 3 (NALP3)) inflammasome and interleukin-1β in HaCaT cells. D. dasycarpus extract did not affect cell viability in basal conditions. The extract significantly reduced oxazolone-induced epidermal swelling compared to untreated animal in the hairless albino mice (ICR mice) model. At the molecular level, Western blot assays indicated that the D. dasycarpus extract attenuated oxazolone-induced activation of apoptosis-associated speck-like protein containing CARD (ASC), procaspase-1, NF-κB and mitogen-activated protein kinase (MAPKs) such as c-Jun N-terminal protein kinase (JNK) and p38. This study demonstrates that D. dasycarpus extract could protect skin cells against oxidative and inflammatory insult by modulating the intracellular levels of ROS, TNF-α, interleukin-1, interleukin-6, NLR family pyrin domain containing 3 (NLRP3) inflammasome generation, antioxidant enzyme activity and cell signaling pathways. D. dasycarpus extract also attenuated the expression of NF-κB in HaCaT keratinocytes and thereby effectively downregulated inflammatory responses in the skin. Furthermore, D. dasycarpus extract alleviated oxazolone-induced damage in mice. Our results suggest the potential application of D. dasycarpus extract in preventing inflammatory processes in dermatitis. View Full-Text
Keywords: D. dasycarpus; ROS; interleukin-1β; interleukin-6; tumor necrosis factor-α; NF-κB; inflammasome D. dasycarpus; ROS; interleukin-1β; interleukin-6; tumor necrosis factor-α; NF-κB; inflammasome
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Chang, T.-M.; Yang, T.-Y.; Niu, Y.-L.; Huang, H.-C. The Extract of D. dasycarpus Ameliorates Oxazolone-Induced Skin Damage in Mice by Anti-Inflammatory and Antioxidant Mechanisms. Antioxidants 2018, 7, 77.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top