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Antioxidants 2018, 7(1), 19; https://doi.org/10.3390/antiox7010019

Interplay between Selenium Levels and Replicative Senescence in WI-38 Human Fibroblasts: A Proteomic Approach

1
Unité Propre de Recherche 3404 (UPR3404), Centre de Génétique Moléculaire (CGM), Centre National de la Recherche Scientifique (CNRS), 91198 Gif-sur-Yvette, France
2
Institut de Biologie Intégrative de la Cellule, Commissariat à L’énergie Atomique et aux Énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Université Paris Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
3
Institut de Génomique Fonctionelle de Lyon (IGFL), Unité Mixte de Recherche 5242 Centre National de la Recherche Scientifique/Ecole Normale Supérieure de Lyon (CNRS/ENS UMR5242), 69007 Lyon, France
4
Centre International de Recherche en Infectiologie (CIRI), 69007 Lyon, France
5
Unité 1111, Institut National de la Santé Et de la Recherche Médicale (INSERM U1111), 69007 Lyon, France
6
Unité Mixte de Recherche 5308, Centre National de la Recherche Scientifique/Ecole Normale Supérieure de Lyon/Université Claude-Bernard-Lyon-I (CNRS/ENS/UCBL1 UMR5308), 69007 Lyon, France
These authors contributed equally to the work.
*
Author to whom correspondence should be addressed.
Received: 31 December 2017 / Revised: 15 January 2018 / Accepted: 17 January 2018 / Published: 20 January 2018
(This article belongs to the Special Issue Selenium and Selenoproteins for Optimal Health)
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Abstract

Selenoproteins are essential components of antioxidant defense, redox homeostasis, and cell signaling in mammals, where selenium is found in the form of a rare amino acid, selenocysteine. Selenium, which is often limited both in food intake and cell culture media, is a strong regulator of selenoprotein expression and selenoenzyme activity. Aging is a slow, complex, and multifactorial process, resulting in a gradual and irreversible decline of various functions of the body. Several cellular aspects of organismal aging are recapitulated in the replicative senescence of cultured human diploid fibroblasts, such as embryonic lung fibroblast WI-38 cells. We previously reported that the long-term growth of young WI-38 cells with high (supplemented), moderate (control), or low (depleted) concentrations of selenium in the culture medium impacts their replicative lifespan, due to rapid changes in replicative senescence-associated markers and signaling pathways. In order to gain insight into the molecular link between selenium levels and replicative senescence, in the present work, we have applied a quantitative proteomic approach based on 2-Dimensional Differential in-Gel Electrophoresis (2D-DIGE) to the study of young and presenescent cells grown in selenium-supplemented, control, or depleted media. Applying a restrictive cut-off (spot intensity ±50% and a p value < 0.05) to the 2D-DIGE analyses revealed 81 differentially expressed protein spots, from which 123 proteins of interest were identified by mass spectrometry. We compared the changes in protein abundance for three different conditions: (i) spots varying between young and presenescent cells, (ii) spots varying in response to selenium concentration in young cells, and (iii) spots varying in response to selenium concentration in presenescent cells. Interestingly, a 72% overlap between the impact of senescence and selenium was observed in our proteomic results, demonstrating a strong interplay between selenium, selenoproteins, and replicative senescence. View Full-Text
Keywords: proteomics; 2-Dimensional Differential in-Gel Electrophoresis (2D-DIGE); selenium; protein abundance; selenoprotein; replicative senescence; WI-38 cells proteomics; 2-Dimensional Differential in-Gel Electrophoresis (2D-DIGE); selenium; protein abundance; selenoprotein; replicative senescence; WI-38 cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hammad, G.; Legrain, Y.; Touat-Hamici, Z.; Duhieu, S.; Cornu, D.; Bulteau, A.-L.; Chavatte, L. Interplay between Selenium Levels and Replicative Senescence in WI-38 Human Fibroblasts: A Proteomic Approach. Antioxidants 2018, 7, 19.

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