In the original publication [1], there was an error in the published **Figure 3G**. Nrf2 and NFkB were detected on the same membrane so they have the same laminin as a control. In fact, among the original bands sent to the journal during submission we show only one laminine. The error occurred in the construction of Figure 3G as the laminin band was overturned by mistake. The correct Figure 3G appears below. The authors state that the scientific conclusions are not affected. This correction has been approved by the Academic Editor. The original publication has also been updated.
Figure 3.
The effects of CV on TLR4, Myd88 and NF-kB pathway expression after DNBS-injection. Immunohistochemistry for TLR4 was evaluated in Sham (A), DNBS (B) and DNBS+CV (C). The results are expressed as number of TLR4+ positive pixel (D). Images are figurative of at least three independent experiments. Western blots for Myd88, IKB-α, and NF-kB. Representative Western blots for cytoplasmic Myd88 (E,E’), IKB-α degradation (F,F’), nuclear NF-kB translocation (G,G’) expression were performed. A demonstrative blot of lysates (5 animals/group), with a densitometric analysis for all animals, is shown (E’,F’,G’). Laminin was used as a control for normalization of NF-κB and Nrf2, which were incubated on the same membrane that was stripped by agitation with 3% glycine, pH 2, blocked in 5% non-fat dried milk/PBS for 1 hour at room temperature, and reincubated with the antibody. Values = means ± SD of 5 animals in each group. *** p < 0.001 vs. Sham; ### p < 0.001 vs. DNBS.
Reference
- Impellizzeri, D.; Fusco, R.; Genovese, T.; Cordaro, M.; D’Amico, R.; Trovato Salinaro, A.; Ontario, M.L.; Modafferi, S.; Cuzzocrea, S.; Di Paola, R.; et al. Coriolus Versicolor Downregulates TLR4/NF-κB Signaling Cascade in Dinitrobenzenesulfonic Acid-Treated Mice: A Possible Mechanism for the Anti-Colitis Effect. Antioxidants 2022, 11, 406. [Google Scholar] [CrossRef] [PubMed]
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