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Antioxidants
Volume 14
Issue 5
10.3390/antiox14050602
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

The Class III Peroxidase Gene Family in Populus simonii: Genome-Wide Identification, Classification, Gene Expression and Functional Analysis

by
Lu Han
1,
Yishuang Ren
1,
Xinru Bi
1,
Guowei Yao
1,
Jinwang Zhang
2,
Hongtao Yuan
2,
Xiaoyu Xie
1,
Junbo Chen
1,
Yunchang Zhang
1,
Sitong Du
1,
Wanying Chen
1,
Kewei Cai
1,* and
Xiyang Zhao
1,*
1
Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural University, Changchun 130118, China
2
Tongliao Forestry and Grassland Science Research Institute, Tongliao 028000, China
*
Authors to whom correspondence should be addressed.
Antioxidants 2025, 14(5), 602; https://doi.org/10.3390/antiox14050602 (registering DOI)
Submission received: 21 February 2025 / Revised: 9 May 2025 / Accepted: 15 May 2025 / Published: 16 May 2025
(This article belongs to the Special Issue Antioxidant Systems in Plants)
Versions Notes

Abstract

Class III peroxidases are plant-specific enzymes that play indispensable roles in catalyzing oxidative–reductive reactions, which are integral to numerous biochemical processes in plants. In this study, we identified 69 members of the class III peroxidase (POD) gene family in the Populus simonii genome and classified them into four subfamilies based on phylogenetic analysis. Chromosomal localization revealed that these PsPOD genes are unevenly distributed across 19 chromosomes, with chromosomes 3 and 7 harboring the highest densities. Conserved domain and motif analyses demonstrated that all PsPOD proteins contain the characteristic peroxidase domain and share highly conserved motif structures. Cis-acting element analysis of promoter regions revealed the presence of numerous regulatory elements associated with light responsiveness, phytohormone signaling, stress responses, and plant growth and development. Transcriptome data showed that the expression of PsPOD genes varies significantly across different tissues and organs and under various stress conditions, suggesting their involvement in both developmental processes and abiotic stress responses. These findings were further validated by qRT-PCR analysis of selected PsPOD genes. Notably, PsPOD45, PsPOD69, PsPOD33, and PsPOD64 were identified as central hub genes in the protein–protein interaction network, making them promising candidates for further functional characterization. Overall, this study provides a comprehensive overview of the PsPOD gene family in P. simonii, laying a solid foundation for future functional studies and offering valuable insights for comparative research in other plant species.
Keywords: Populus simonii; class III peroxidase (POD); expression pattern; protein–protein interaction Populus simonii; class III peroxidase (POD); expression pattern; protein–protein interaction

Share and Cite

MDPI and ACS Style

Han, L.; Ren, Y.; Bi, X.; Yao, G.; Zhang, J.; Yuan, H.; Xie, X.; Chen, J.; Zhang, Y.; Du, S.; et al. The Class III Peroxidase Gene Family in Populus simonii: Genome-Wide Identification, Classification, Gene Expression and Functional Analysis. Antioxidants 2025, 14, 602. https://doi.org/10.3390/antiox14050602

AMA Style

Han L, Ren Y, Bi X, Yao G, Zhang J, Yuan H, Xie X, Chen J, Zhang Y, Du S, et al. The Class III Peroxidase Gene Family in Populus simonii: Genome-Wide Identification, Classification, Gene Expression and Functional Analysis. Antioxidants. 2025; 14(5):602. https://doi.org/10.3390/antiox14050602

Chicago/Turabian Style

Han, Lu, Yishuang Ren, Xinru Bi, Guowei Yao, Jinwang Zhang, Hongtao Yuan, Xiaoyu Xie, Junbo Chen, Yunchang Zhang, Sitong Du, and et al. 2025. "The Class III Peroxidase Gene Family in Populus simonii: Genome-Wide Identification, Classification, Gene Expression and Functional Analysis" Antioxidants 14, no. 5: 602. https://doi.org/10.3390/antiox14050602

APA Style

Han, L., Ren, Y., Bi, X., Yao, G., Zhang, J., Yuan, H., Xie, X., Chen, J., Zhang, Y., Du, S., Chen, W., Cai, K., & Zhao, X. (2025). The Class III Peroxidase Gene Family in Populus simonii: Genome-Wide Identification, Classification, Gene Expression and Functional Analysis. Antioxidants, 14(5), 602. https://doi.org/10.3390/antiox14050602

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