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Open AccessArticle

Resveratrol Prevents GLUT3 Up-Regulation Induced by Middle Cerebral Artery Occlusion

1
Laboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía “Manuel Velasco Suárez”, Insurgentes Sur #3877, Mexico City 14269, Mexico
2
Laboratorio de Formación Reticular, Instituto Nacional de Neurología y Neurocirugía “Manuel Velasco Suárez”, Insurgentes Sur #3877, Mexico City 14269, Mexico
3
Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía “Manuel Velasco Suárez”, Insurgentes Sur #3877, Mexico City 14269, Mexico
4
Unidad del Bioterio, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico
5
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico
*
Author to whom correspondence should be addressed.
Brain Sci. 2020, 10(9), 651; https://doi.org/10.3390/brainsci10090651
Received: 20 August 2020 / Revised: 7 September 2020 / Accepted: 18 September 2020 / Published: 20 September 2020
(This article belongs to the Special Issue Biochemical Biomarkers and Neurodegenerative Diseases)
Glucose transporter (GLUT)3 up-regulation is an adaptive response activated to prevent cellular damage when brain metabolic energy is reduced. Resveratrol is a natural polyphenol with anti-oxidant and anti-inflammatory features that protects neurons against damage induced in cerebral ischemia. Since transcription factors sensitive to oxidative stress and inflammation modulate GLUT3 expression, the purpose of this work was to assess the effect of resveratrol on GLUT3 expression levels after ischemia. Male Wistar rats were subjected to 2 h of middle cerebral artery occlusion (MCAO) followed by different times of reperfusion. Resveratrol (1.9 mg/kg; i. p.) was administered at the onset of the restoration of the blood flow. Quantitative-PCR and Western blot showed that MCAO provoked a substantial increase in GLUT3 expression in the ipsilateral side to the lesion of the cerebral cortex. Immunofluorescence assays indicated that GLUT3 levels were upregulated in astrocytes. Additionally, an important increase in GLUT3 occurred in other cellular types (e.g., damaged neurons, microglia, or infiltrated macrophages). Immunodetection of the microtubule-associated protein 2 (MAP2) showed that MCAO induced severe damage to the neuronal population. However, the administration of resveratrol at the time of reperfusion resulted in injury reduction. Resveratrol also prevented the MCAO-induced increase of GLUT3 expression. In conclusion, resveratrol protects neurons from damage induced by ischemia and prevents GLUT3 upregulation in the damaged brain that might depend on AMPK activation. View Full-Text
Keywords: GLUT3; cerebral ischemia; MCAO; resveratrol; astrocytes; AMPK GLUT3; cerebral ischemia; MCAO; resveratrol; astrocytes; AMPK
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Gutiérrez Aguilar, G.F.; Alquisiras-Burgos, I.; Franco-Pérez, J.; Pineda-Ramírez, N.; Ortiz-Plata, A.; Torres, I.; Pedraza-Chaverri, J.; Aguilera, P. Resveratrol Prevents GLUT3 Up-Regulation Induced by Middle Cerebral Artery Occlusion. Brain Sci. 2020, 10, 651.

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