Next Article in Journal
Shear Strength Prediction Equations and Experimental Study of High Strength Steel Fiber-Reinforced Concrete Beams with Different Shear Span-to-Depth Ratios
Previous Article in Journal
Time Shared Optical Network (TSON): A Programmable Network Edge Solution for Multi-Access Support
Open AccessArticle

Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry

1
Department of Pharmaceutical Analysis, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania
2
Department of Proteomics and Metabolomics, MedFuture-Research Center for Advanced Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, Louis Pasteur Street 4-6, Gh. Marinescu Street 23, 400349 Cluj-Napoca, Romania
3
Department of Toxicology, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
Appl. Sci. 2019, 9(22), 4789; https://doi.org/10.3390/app9224789
Received: 18 October 2019 / Revised: 1 November 2019 / Accepted: 6 November 2019 / Published: 9 November 2019
(This article belongs to the Special Issue Smart Nano-Biointerfaces for Theranostics)
The interactions of nanoparticles with living organisms are driven by an interface called the protein corona. This interface is formed when nanoparticles are introduced in biological milieu and proteins are adsorbed at nanoparticles’ surfaces. Understanding the factors that are responsible for the formation and the composition of the protein corona could reveal mechanistic insights that are involved in the interaction of nanoparticles with biological structures. Multiple studies have qualitatively described the protein corona, but just a few have proposed quantification methods, especially for gold nanoparticles. Using bovine serum albumin conjugated with fluorescein-5-isothiocyanate as a model protein, we developed a fluorescent-based quantification method for gold nanoparticles’ protein coronas. The impact of nanoparticle size and surface chemistry was studied, and our research emphasizes that size and surface chemistry are determinant factors: Bigger nanoparticles and amino-modified surface chemistry are responsible for higher protein adsorption compared to smaller ones and carboxyl- or methoxy-modified surface chemistry. The proposed method can be used to complete the full picture of the interactions of nanoparticles with biological milieu and to describe the parameters which govern these interactions for the better development of nanomedicines. View Full-Text
Keywords: gold nanoparticles; protein corona; PEG coating; colloidal stability gold nanoparticles; protein corona; PEG coating; colloidal stability
Show Figures

Figure 1

MDPI and ACS Style

Nicoară, R.; Ilieș, M.; Uifălean, A.; Iuga, C.A.; Loghin, F. Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry. Appl. Sci. 2019, 9, 4789.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop