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Appl. Sci. 2018, 8(2), 235;

Large Scale Imaging by Fine Spatial Alignment of Multi-Scanning Data with Gel Cube Device

NanoSquare Research Institute, Osaka Prefecture University, Osaka 599-8570, Japan
Department of Bioscience and Informatics, Osaka Prefecture University, Osaka 599-8570, Japan
Department of Biological Functions Engineering, Kyushu Institute of Technology, Kitakyushu 808-0196, Japan
Author to whom correspondence should be addressed.
Received: 10 January 2018 / Revised: 31 January 2018 / Accepted: 1 February 2018 / Published: 4 February 2018
(This article belongs to the Special Issue Microfluidics in Biology and Medicine)
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In vitro three-dimensional (3D) culturing is considered essential in many biological fields. However, the imaging of developed 3D formations is often difficult, especially if the size of the sample is relatively large. The z-resolution of fluorescent imaging is low using low magnification lenses (4× and 10×) due to large focal depths. This paper describes 3D culture platform enabling large scale 3D imaging by fine spatial alignment of the image dataset obtained from multiple directions. A gel cube device was employed to conduct the multi-scanning and then a self-fluorescent microstructure in a cubic frame allows us spatially align image dataset within a few pixels. By synthesizing data from multiple scans, the platform enables us to visualize millimeter-sized 3D sample structure and individual cellular actin filaments at the same time. Millimeter depth imaging of a developed bronchial tree was achieved with high z-resolution. The device, which is applicable to most microscopy systems, can enhance the image quality without modifying current systems. View Full-Text
Keywords: 3D culture; 3D image; multi-directional imaging 3D culture; 3D image; multi-directional imaging

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hagiwara, M.; Nobata, R.; Kawahara, T. Large Scale Imaging by Fine Spatial Alignment of Multi-Scanning Data with Gel Cube Device. Appl. Sci. 2018, 8, 235.

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