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Open AccessArticle

Pneumatically Actuated Microfluidic Platform for Reconstituting 3D Vascular Tissue Compression

1
Department of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-742, Korea
2
Bioengineering, Seoul National University, Seoul 151-742, Korea
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(6), 2027; https://doi.org/10.3390/app10062027
Received: 21 January 2020 / Revised: 26 February 2020 / Accepted: 3 March 2020 / Published: 17 March 2020
(This article belongs to the Special Issue Biomaterials and Biofabrication)
In vivo, blood vessels constitutively experience mechanical stresses exerted by adjacent tissues and other structural elements. Vascular collapse, a structural failure of vascular tissues, may stem from any number of possible compressive forces ranging from injury to tumor growth and can promote inflammation. In particular, endothelial cells are continuously exposed to varying mechanical stimuli, internally and externally, resulting in blood vessel deformation and injury. This study proposed a method to model biomechanical-stimuli-induced blood vessel compression in vitro within a polydimethylsiloxane (PDMS) microfluidic 3D microvascular tissue culture platform with an integrated pneumatically actuated compression mechanism. 3D microvascular tissues were cultured within the device. Histological reactions to compressive forces were quantified and shown to be the following: live/dead assays indicated the presence of a microvascular dead zone within high-stress regions and reactive oxygen species (ROS) quantification exhibited a stress-dependent increase. Fluorescein isothiocyanate (FITC)-dextran flow assays showed that compressed vessels developed structural failures and increased leakiness; finite element analysis (FEA) corroborated the experimental data, indicating that the suggested model of vascular tissue deformation and stress distribution was conceptually sound. As such, this study provides a powerful and accessible in vitro method of modeling microphysiological reactions of microvascular tissues to compressive stress, paving the way for further studies into vascular failure as a result of external stress. View Full-Text
Keywords: blood vessel compression; biomechanical stress; microfluidic chip; pneumatically acutuated valve; perfusable blood vessel blood vessel compression; biomechanical stress; microfluidic chip; pneumatically acutuated valve; perfusable blood vessel
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MDPI and ACS Style

Ahn, J.; Lee, H.; Kang, H.; Choi, H.; Son, K.; Yu, J.; Lee, J.; Lim, J.; Park, D.; Cho, M.; Jeon, N.L. Pneumatically Actuated Microfluidic Platform for Reconstituting 3D Vascular Tissue Compression. Appl. Sci. 2020, 10, 2027. https://doi.org/10.3390/app10062027

AMA Style

Ahn J, Lee H, Kang H, Choi H, Son K, Yu J, Lee J, Lim J, Park D, Cho M, Jeon NL. Pneumatically Actuated Microfluidic Platform for Reconstituting 3D Vascular Tissue Compression. Applied Sciences. 2020; 10(6):2027. https://doi.org/10.3390/app10062027

Chicago/Turabian Style

Ahn, Jungho; Lee, Hyeok; Kang, Habin; Choi, Hyeri; Son, Kyungmin; Yu, James; Lee, Jungseub; Lim, Jungeun; Park, Dohyun; Cho, Maenghyo; Jeon, Noo L. 2020. "Pneumatically Actuated Microfluidic Platform for Reconstituting 3D Vascular Tissue Compression" Appl. Sci. 10, no. 6: 2027. https://doi.org/10.3390/app10062027

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