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Animals 2019, 9(4), 142; https://doi.org/10.3390/ani9040142

Identification of Ovine KRTAP28-1 and Its Association with Wool Fibre Diameter

1
Agricultural College, Ningxia University, Yinchuan 750021, China
2
College of Animal Science and Technology, Hebei North University, Zhangjiakou, Hebei 075131, China
3
Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 25 February 2019 / Revised: 25 March 2019 / Accepted: 29 March 2019 / Published: 2 April 2019
(This article belongs to the Section Animal Genetics and Genomics)
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Simple Summary

Keratin-associated proteins (KAPs) are fundamental components of wool and hair fibres. They are split into three broad groups: the high sulphur (HS), the ultra-high sulphur (UHS) and the high glycine-tyrosine (HGT) KAPs. KRTAP25-1 encodes a HS-KAP protein and the gene has recently been identified in humans. Here, we report the absence of a KRTAP25-1 in sheep, and we describe a new HS-KRTAP (named KRTAP28-1) in the chromosome region where KRTAP25-1 was expected to be found. Six variants (AF) of KRTAP28-1 containing eight single nucleotide polymorphisms (SNPs) and a TG dinucleotide repeat polymorphism were detected. One SNP was located upstream of the start codon and all the others were non-synonymous SNPs, including a nonsense SNP. The TG repeat polymorphism would lead to a reading frame shift at the carboxyl-terminal end. The association of KRTAP28-1 with wool traits was investigated with 383 Southdown × Merino-cross lambs from seven sire lines. Of the four genotypes with a frequency over 5%, lambs of genotypes AB and BD produced wool of a smaller mean fibre diameter (MFD) than lambs of genotype BC. This shows that KRTAP28-1 is associated with a key wool trait, and variation in this gene might therefore have value as a marker for improving that trait.

Abstract

Keratin-associated proteins (KAPs) are a diverse group of proteins and form a matrix that cross-links keratin intermediate filaments in hair and wool fibres. From over 100 KAP genes (KRTAPs) identified in mammalian species, KRTAP25-1 is a high sulphur (HS)-KAP gene, which has recently been described in humans. Here, we report the absence of KRTAP25-1 in sheep, and describe a new HS-KRTAP (named KRTAP28-1) in the chromosome region where KRTAP25-1 was expected to be found. Six variants (AF) of KRTAP28-1 containing eight single nucleotide polymorphisms (SNPs) and a TG repeat polymorphism were detected. One was positioned 30 bp upstream of the transcription start codon and all the others were non-synonymous SNPs, including a nonsense SNP. The TG repeat polymorphism would lead to a reading frame shift at the carboxyl-terminal end. The effect of KRTAP28-1 on wool traits was investigated with 383 Southdown × Merino-cross lambs from seven sire lines. Of the four genotypes with a frequency of over 5%, lambs of genotypes AB and BD produced wool of a smaller MFD than lambs of genotype BC. This shows that KRTAP28-1 is associated with wool fibre diameter, and that variation in this gene might have potential for use as a gene marker for reducing wool fibre diameter. View Full-Text
Keywords: sheep; keratin-associated protein KAP28-1 gene (KRTAP28-1); polymerase chain reaction (PCR); single-stranded conformational polymorphism (SSCP); variation; wool fibre diameter sheep; keratin-associated protein KAP28-1 gene (KRTAP28-1); polymerase chain reaction (PCR); single-stranded conformational polymorphism (SSCP); variation; wool fibre diameter
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MDPI and ACS Style

Bai, L.; Wang, J.; Zhou, H.; Gong, H.; Tao, J.; Hickford, J.G.H. Identification of Ovine KRTAP28-1 and Its Association with Wool Fibre Diameter. Animals 2019, 9, 142.

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