Prevalence and Molecular Characterization of Mycoplasma Species, Pasteurella multocida, and Staphylococcus aureus Isolated from Calves with Respiratory Manifestations
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Study Area
2.3. Animals and Samples Collection
2.4. Phenotypic Isolation and Identification of Mycoplasma spp., S. aureus, and P. multocida
2.5. Molecular Confirmation of Mycoplasma spp., S. aureus, and P. multocida Strains by PCR
2.6. Sequencing and Phylogenetic Analysis
2.7. Data Submission
3. Results
3.1. Prevalence of M. bovis, M. bovigenitalium, P. multocida, and S. aureus Recovered from Calves with Respiratory Signs
3.2. Molecular Detection of M. bovis, M. bovigenitalium, P. multocida, and S. aureus Isolated from Calves with Respiratory Signs
3.3. Sequence Analysis of M. bovis, M. bovigenitalium, P. multocida, and S. aureus Isolated from Calves with Respiratory Signs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Organism | Gene | Primer Name | Primer Sequence (5′-3′) | Anneal. Temp. | Amplicon Size | References |
---|---|---|---|---|---|---|
Class Mollicutes | 16S rRNA | MW28-F MW29-R | CCAGACTCCTACGGGAGGCA TGCGAGCATACTACTCAGGC | 55 °C | 580 bp | [29] |
M. bovis | mb-mp 81 | Mb-mp 81 F Mb-mp 81 R | TATTGGATCAACTGCTGGAT AGATGCTCCACTTATCTTAG | 54 °C | 447 bp | [30] |
M. bovigenitalium | 16S rRNA | Mbg F Mbg R | CGTAGATGCCGCATGGCATTTACGG CATTCAATATAGTGGCATTTCCTAC | 60 °C | 321 bp | [31] |
P. multocida | 16S rRNA | KMT1T7 KMT1SP6 | GCTGTAAACGAACTCGCCAC ATCCGCTATTTACCCAGTGG | 64 °C | 460 bp | [32] |
S. aureus | 16S rRNA | Sau 327 Sau 1645 | GGA CGA CAT TAG ACG AAT CA CGG GCA CCT ATT TTC TAT CT | 55 °C | 1318 bp | [33] |
S. aureus | coa | G2 G3 | ACCACAAGGTACTGAATCAACG TGCTTTCGATTGTTCGATGC | 55 °C | 987 bp | [34] |
S. aureus | nuc | Nuc | GCGATTGATGGTGATACGGTT AGCCAAGCCTTGGAACTAAAGC | 55 °C | 270 bp | [35] |
No. of Calves Examined | No. of Calves Showing Respiratory Signs | M. bovis | M. bovigenitalium | S. aureus | P. multocida | |||||
---|---|---|---|---|---|---|---|---|---|---|
No. | % | No. | % | No. | % | No. | % | No. | % | |
200 | 60 | 30 | 5 | 8.33 | 3 | 5 | 3 | 5 | 3 | 5 |
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Hashem, Y.M.; Mousa, W.S.; Abdeen, E.E.; Abdelkhalek, H.M.; Nooruzzaman, M.; El-Askary, A.; Ismail, K.A.; Megahed, A.M.; Abdeen, A.; Soliman, E.A.; et al. Prevalence and Molecular Characterization of Mycoplasma Species, Pasteurella multocida, and Staphylococcus aureus Isolated from Calves with Respiratory Manifestations. Animals 2022, 12, 312. https://doi.org/10.3390/ani12030312
Hashem YM, Mousa WS, Abdeen EE, Abdelkhalek HM, Nooruzzaman M, El-Askary A, Ismail KA, Megahed AM, Abdeen A, Soliman EA, et al. Prevalence and Molecular Characterization of Mycoplasma Species, Pasteurella multocida, and Staphylococcus aureus Isolated from Calves with Respiratory Manifestations. Animals. 2022; 12(3):312. https://doi.org/10.3390/ani12030312
Chicago/Turabian StyleHashem, Youserya M., Walid S. Mousa, Eman E. Abdeen, Hanaa M. Abdelkhalek, Mohammed Nooruzzaman, Ahmad El-Askary, Khadiga A. Ismail, Ayman M. Megahed, Ahmed Abdeen, Enas A. Soliman, and et al. 2022. "Prevalence and Molecular Characterization of Mycoplasma Species, Pasteurella multocida, and Staphylococcus aureus Isolated from Calves with Respiratory Manifestations" Animals 12, no. 3: 312. https://doi.org/10.3390/ani12030312
APA StyleHashem, Y. M., Mousa, W. S., Abdeen, E. E., Abdelkhalek, H. M., Nooruzzaman, M., El-Askary, A., Ismail, K. A., Megahed, A. M., Abdeen, A., Soliman, E. A., & Wareth, G. (2022). Prevalence and Molecular Characterization of Mycoplasma Species, Pasteurella multocida, and Staphylococcus aureus Isolated from Calves with Respiratory Manifestations. Animals, 12(3), 312. https://doi.org/10.3390/ani12030312