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Article

Comparative Study of Biological Characteristics, and Osteoblast Differentiation of Mesenchymal Stem Cell Established from Camelus dromedarius Skeletal Muscle, Dermal Skin, and Adipose Tissues

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UAE Biotech Research Center, Abu Dhabi 30310, United Arab Emirates
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Hilli E.T. Cloning and Surgical Centre Presidential Camels and Camel Racing Affairs, Al-Ain P.O. Box 17292, Unitied Arab Emirates
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Author to whom correspondence should be addressed.
Academic Editor: Ioanna-Katerina Aggeli
Animals 2021, 11(4), 1017; https://doi.org/10.3390/ani11041017
Received: 24 February 2021 / Revised: 31 March 2021 / Accepted: 2 April 2021 / Published: 4 April 2021
(This article belongs to the Section Veterinary Clinical Studies)
Mesenchymal stem cells (MSCs) can be isolated in various types of tissues and exhibit different characteristics. In this study, MSCs were established from skeletal muscle, dermal skin, and adipose tissue from a single Camelus dromedarius donor. We also identified an efficient source for osteoblast differentiation and analyzed biological characteristics.
Mesenchymal stem cells (MSCs) showed in vitro mesoderm-lineage differentiation and self-renewal capacity. However, no comparative study was reported on the biological characteristics of stem cells derived from skeletal muscle (SM-MSCs), dermal skin (DS-MSCs), and adipose tissues (A-MSCs) from a single donor in camels. The present study aimed to evaluate the influence of MSCs source on stem cell characteristics. We evaluated proliferation capacity and mesoderm-lineage differentiation potential from SM-MSCs, DS-MSCs, and A-MSCs. They showed spindle-like morphology after homogenization. The proliferation ability was not significantly difference in any of the groups. Furthermore, the portion of the cell cycle and expression of pluripotent markers (Oct4, Sox2, and Nanog) were similar in all cell lines at passage 3. The differentiation capacity of A-MSCs into adipocytes was significantly higher than that of SM-MSCs and DS-MSCs. However, the osteoblast differentiation capacity of A-MSCs was significantly lower than that of SM-MSCs and DS-MSCs. Additionally, after osteoblast differentiation, the alkaline phosphatase (ALP) activity and calcium content significantly decreased in A-MSCs compared to SM-MSCs and DS-MSCs. To the best of our knowledge, we primarily established MSCs from the single camel and demonstrated their comparative characteristics, including expression of pluripotent factors and proliferation, and in vitro differentiation capacity into adipocytes and osteoblasts. View Full-Text
Keywords: mesenchymal stem cells; Camelus dromedarius; skeletal muscle; dermal skin; adipose tissue; differentiation mesenchymal stem cells; Camelus dromedarius; skeletal muscle; dermal skin; adipose tissue; differentiation
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MDPI and ACS Style

Son, Y.-B.; Jeong, Y.I.; Jeong, Y.W.; Hossein, M.S.; Tinson, A.; Singh, K.K.; Hwang, W.S. Comparative Study of Biological Characteristics, and Osteoblast Differentiation of Mesenchymal Stem Cell Established from Camelus dromedarius Skeletal Muscle, Dermal Skin, and Adipose Tissues. Animals 2021, 11, 1017. https://doi.org/10.3390/ani11041017

AMA Style

Son Y-B, Jeong YI, Jeong YW, Hossein MS, Tinson A, Singh KK, Hwang WS. Comparative Study of Biological Characteristics, and Osteoblast Differentiation of Mesenchymal Stem Cell Established from Camelus dromedarius Skeletal Muscle, Dermal Skin, and Adipose Tissues. Animals. 2021; 11(4):1017. https://doi.org/10.3390/ani11041017

Chicago/Turabian Style

Son, Young-Bum, Yeon Ik Jeong, Yeon Woo Jeong, Mohammad Shamim Hossein, Alex Tinson, Kuhad Kuldip Singh, and Woo Suk Hwang. 2021. "Comparative Study of Biological Characteristics, and Osteoblast Differentiation of Mesenchymal Stem Cell Established from Camelus dromedarius Skeletal Muscle, Dermal Skin, and Adipose Tissues" Animals 11, no. 4: 1017. https://doi.org/10.3390/ani11041017

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