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Article

Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park

L.K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy, Podolsk Municipal District, Moscow Region, 142132 Podolsk, Russia
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Authors to whom correspondence should be addressed.
Andrey Rodionov and Tatiana Deniskova contributed equally to this work.
Academic Editors: Stephen F. Pires and George Olah
Animals 2021, 11(12), 3416; https://doi.org/10.3390/ani11123416
Received: 7 October 2021 / Revised: 18 November 2021 / Accepted: 28 November 2021 / Published: 30 November 2021
(This article belongs to the Special Issue Wildlife Crime: Issues and Promising Solutions)
DNA molecular techniques, including multiple microsatellite analysis and genome-wide SNP-genotyping, were used to unlock and prove the poaching of wild goats (Capra caucasica) in an area of the Caucasian mountains in Russia.
Poaching is one of the major types of wildlife crime in Russia. Remnants of goats (presumably the wild endemic species, the Caucasian tur) were found in an area of the Caucasian mountains. The case study involves a suspected poacher whose vehicle was found to have two duffel bags containing pieces of a carcass, which he claimed was that of a goat from his flock. The aim of the forensic genetic analysis for this case was to (i) establish individual identity and (ii) perform species identification. DNA typing based on fourteen microsatellites revealed that STR-genotypes generated from pieces of evidence found at crime scene fully matched those obtained from the evidence seized from the suspect. The results of genome-wide SNP-genotyping, using Illumina Goat SNP50 BeadChip, provided evidence that the poached animal was a wild Caucasian tur (Capra caucasica). Thus, based on comprehensive molecular genetic analysis, evidence of poaching was obtained and sent to local authorities. To our knowledge, this case study is the first to attempt to use DNA chips in wildlife forensics of ungulates. View Full-Text
Keywords: poaching; wild goat; single-nucleotide polymorphisms; microsatellites; evidence; DNA typing poaching; wild goat; single-nucleotide polymorphisms; microsatellites; evidence; DNA typing
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MDPI and ACS Style

Rodionov, A.; Deniskova, T.; Dotsev, A.; Volkova, V.; Petrov, S.; Kharzinova, V.; Koshkina, O.; Abdelmanova, A.; Solovieva, A.; Shakhin, A.; Bardukov, N.; Zinovieva, N. Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park. Animals 2021, 11, 3416. https://doi.org/10.3390/ani11123416

AMA Style

Rodionov A, Deniskova T, Dotsev A, Volkova V, Petrov S, Kharzinova V, Koshkina O, Abdelmanova A, Solovieva A, Shakhin A, Bardukov N, Zinovieva N. Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park. Animals. 2021; 11(12):3416. https://doi.org/10.3390/ani11123416

Chicago/Turabian Style

Rodionov, Andrey, Tatiana Deniskova, Arsen Dotsev, Valeria Volkova, Sergey Petrov, Veronika Kharzinova, Olga Koshkina, Alexandra Abdelmanova, Anastasia Solovieva, Alexey Shakhin, Nikolay Bardukov, and Natalia Zinovieva. 2021. "Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park" Animals 11, no. 12: 3416. https://doi.org/10.3390/ani11123416

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