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Article

Metabolic Profiling of Plasma in Different Calving Body Condition Score Cows Using an Untargeted Liquid Chromatography-Mass Spectrometry Metabolomics Approach

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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Animals 2020, 10(9), 1709; https://doi.org/10.3390/ani10091709
Received: 4 August 2020 / Revised: 10 September 2020 / Accepted: 15 September 2020 / Published: 21 September 2020
(This article belongs to the Special Issue Dairy Cow Health and Welfare)
Over-conditioning around calving predisposes cows to a higher risk of postpartum metabolic disorders and diseases. Using untargeted liquid chromatography-mass spectrometry, seven metabolites were found that were significantly correlated to high body condition, as well as to classic metabolic parameters indicating changes in lipid metabolism and inflammation. These seven metabolites include six distinct lysophosphatidylcholines and a phosphatidylethanolamine. By investigating the depth of metabolic changes in the plasma of over-conditioned fresh cows, this study may provide a metabolic basis for the diseases associated with over-conditioning.
This study was undertaken to identify metabolite differences in plasma of dairy cows with a normal or high calving body condition score (CBCS), using untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics. Sixteen multiparous dairy cows were assigned to one of two groups based on CBCS (0 to 5 scale): Normal group (NBCS, 3.25 ≤ BCS ≤ 3.5, n = 8), and high BCS group (HBCS, BCS ≥ 4, n = 8). Plasma samples were collected for metabolomics analysis and evaluation of biomarkers of lipid metabolism (nonesterified fatty acid (NEFA) and β-hydroxybutyrate (BHB)), and cytokines (leptin, adiponectin, tumor necrosis factor–α (TNF-α) and interleukin 6 (IL-6)). A total of 23 differential metabolites were identified, and functional analyses were performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Among these metabolites, the concentrations of six lysophosphatidylcholines and one phosphatidylethanolamine, were lower in the HBCS group than in the NBCS group (p < 0.01). Furthermore, these metabolites were involved in these four pathways, among others: glycerophospholipid metabolism, retrograde endocannabinoid signaling, autophagy, and glycosylphosphatidylinositol (GPI)-anchor biosynthesis (p < 0.05). In addition, plasma concentrations of leptin (p = 0.06) and TNF-α (p = 0.08) tended to be greater while adiponectin (p = 0.09) lower in HBCS cows than in NBCS cows. The concentrations of NEFA, BHB, or IL-6 did not differ between NBCS and HBCS groups. More importantly, based on the results of the Spearman’s correlation analysis, the seven important metabolites were negatively correlated with indices of lipid metabolisms, proinflammatory cytokines, and leptin, but positively correlated with adiponectin. These results demonstrate that CBCS has a measurable impact on the plasma metabolic profile, even when NEFA and BHB are not different. In addition, the identified differential metabolites were significantly correlated to lipid metabolism and inflammation in the over-conditioned fresh cows, which are expected to render a metabolic basis for the diseases associated with over-conditioned dry cows. View Full-Text
Keywords: calving body condition score; metabolomics; lysophosphatidylcholines; phosphatidylethanolamine; cow calving body condition score; metabolomics; lysophosphatidylcholines; phosphatidylethanolamine; cow
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MDPI and ACS Style

Wang, J.; Zhang, C.; Zhao, Q.; Li, C.; Jin, S.; Gu, X. Metabolic Profiling of Plasma in Different Calving Body Condition Score Cows Using an Untargeted Liquid Chromatography-Mass Spectrometry Metabolomics Approach. Animals 2020, 10, 1709. https://doi.org/10.3390/ani10091709

AMA Style

Wang J, Zhang C, Zhao Q, Li C, Jin S, Gu X. Metabolic Profiling of Plasma in Different Calving Body Condition Score Cows Using an Untargeted Liquid Chromatography-Mass Spectrometry Metabolomics Approach. Animals. 2020; 10(9):1709. https://doi.org/10.3390/ani10091709

Chicago/Turabian Style

Wang, Jian, Chuang Zhang, Qingyao Zhao, Congcong Li, Shuang Jin, and Xianhong Gu. 2020. "Metabolic Profiling of Plasma in Different Calving Body Condition Score Cows Using an Untargeted Liquid Chromatography-Mass Spectrometry Metabolomics Approach" Animals 10, no. 9: 1709. https://doi.org/10.3390/ani10091709

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