Aquaporin 1 (AQP1) Expression in Healthy Dog Tears
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Experimental Protocol
2.3. Complete Blood Count and Serum Biochemistry
2.4. Tear Sample Collection
2.5. Tear Protein Analysis
2.6. AQP1 Expression in Tears by Western Blotting
2.7. Statistical Analysis
3. Results
3.1. Ocular Parameters
3.2. Analysis of Dog Tear Parameters (Volume and Total Protein Content)
3.3. AQP1 Expression by Western Blotting
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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ID | Breed | BW (kg) | Sex | Age (years) |
---|---|---|---|---|
1 | Labrador | 32 | F | 2 |
2 | Golden Retriever | 34 | M | 5 |
3 | Kurzhaar | 28 | M | 3 |
4 | Mix breed | 10 | F | 2 |
5 | Whippet | 17 | M | 6 |
6 | Whippet | 19 | M | 7 |
7 | Mix breed | 30 | M | 3 |
8 | Mix breed | 31 | M | 4 |
9 | Mix breed | 25 | F | 5 |
10 | Jack Russel | 13 | M | 3 |
11 | Beagle | 17 | M | 6 |
12 | Mix breed | 19 | M | 1 |
13 | Mix breed | 14 | F | 3 |
14 | Border Collie | 15 | F | 2 |
15 | Pointer | 23 | M | 1 |
Order of Testing | Study Procedure |
---|---|
Day 0 | |
1 | Visual examination |
2 | Slit lamp examination of eyelids, cornea, tear meniscus and conjunctiva |
3 | Shirmer tear test (STT-1) and intraocular pressure (IOP) (both eyes) |
Day 1 | |
1 | Tear sample collection: by randomly using Schirmer tear strips (STS) in one eye and ophthalmic sponges (OS) in the other |
2 | Analysis of parameters (volume and total protein content) |
3 | Evaluation of aquaporin 1 (AQP1) expression by Western blotting |
Day 2 | |
1 | Assessment of tear film break up time (BUT) |
2 | Fluorescein test corneal staining |
3 | Lyssamine green corneal staining |
ID | STT-1 (RE) (mm/min) | STT-1(LE) (mm/min) | IOP (RE) (mm/Hg) | IOP (LE) (mm/Hg) | BUT (RE) (s) | BUT (LE) (s) |
---|---|---|---|---|---|---|
1 | 15 | 18 | 15 | 20 | 18 | 19 |
2 | 20 | 22 | 16 | 22 | 19 | 17 |
3 | 22 | 20 | 21 | 14 | 18 | 18 |
4 | 15 | 15 | 14 | 17 | 21 | 20 |
5 | 30 | 24 | 22 | 19 | 20 | 21 |
6 | 13 | 16 | 14 | 20 | 19 | 18 |
7 | 17 | 24 | 16 | 22 | 18 | 19 |
8 | 18 | 20 | 23 | 21 | 20 | 22 |
9 | 25 | 25 | 22 | 23 | 18 | 18 |
10 | 23 | 17 | 20 | 22 | 18 | 19 |
11 | 23 | 20 | 17 | 17 | 20 | 19 |
12 | 26 | 26 | 19 | 21 | 21 | 20 |
13 | 20 | 15 | 23 | 19 | 20 | 19 |
14 | 15 | 20 | 14 | 18 | 20 | 21 |
15 | 19 | 22 | 14 | 17 | 18 | 18 |
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Lamagna, B.; Ciaramella, P.; Lamagna, F.; Di Loria, A.; Brunetti, A.; Pelagalli, A. Aquaporin 1 (AQP1) Expression in Healthy Dog Tears. Animals 2020, 10, 820. https://doi.org/10.3390/ani10050820
Lamagna B, Ciaramella P, Lamagna F, Di Loria A, Brunetti A, Pelagalli A. Aquaporin 1 (AQP1) Expression in Healthy Dog Tears. Animals. 2020; 10(5):820. https://doi.org/10.3390/ani10050820
Chicago/Turabian StyleLamagna, Barbara, Paolo Ciaramella, Francesco Lamagna, Antonio Di Loria, Arturo Brunetti, and Alessandra Pelagalli. 2020. "Aquaporin 1 (AQP1) Expression in Healthy Dog Tears" Animals 10, no. 5: 820. https://doi.org/10.3390/ani10050820
APA StyleLamagna, B., Ciaramella, P., Lamagna, F., Di Loria, A., Brunetti, A., & Pelagalli, A. (2020). Aquaporin 1 (AQP1) Expression in Healthy Dog Tears. Animals, 10(5), 820. https://doi.org/10.3390/ani10050820