Next Article in Journal
Diverse Genotypes and Species of Cryptosporidium in Wild Rodent Species from the West Coast of the USA and Implications for Raw Produce Safety and Microbial Water Quality
Next Article in Special Issue
Elevated Incidences of Antimicrobial Resistance and Multidrug Resistance in the Maumee River (Ohio, USA), a Major Tributary of Lake Erie
Previous Article in Journal
Characterisation of Phage Susceptibility Variation in Salmonellaenterica Serovar Typhimurium DT104 and DT104b
Previous Article in Special Issue
Culture-Independent Genotyping, Virulence and Antimicrobial Resistance Gene Identification of Staphylococcus aureus from Orthopaedic Implant-Associated Infections
Article

Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries

1
CEITEC VETUNI, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic
2
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic
3
Biomedical Centre, Charles University, 32300 Pilsen, Czech Republic
4
Department of Microbiology, University Hospital of Larissa, 41110 Larissa, Greece
5
Faculty of Medicine in Pilsen, Charles University, 30605 Pilsen, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Sangryeol Ryu
Microorganisms 2021, 9(4), 866; https://doi.org/10.3390/microorganisms9040866
Received: 27 March 2021 / Revised: 14 April 2021 / Accepted: 15 April 2021 / Published: 17 April 2021
Poultry represents a common source of bacteria with resistance to antibiotics including the critically important ones. Selective cultivation using colistin, cefotaxime and meropenem was performed for 66 chicken samples coming from 12 farms in Paraguay while two breeding companies supplied the farms. A total of 62 Escherichia coli and 22 Klebsiella pneumoniae isolates were obtained and representative isolates were subjected to whole-genome sequencing. Relatively high prevalence of phylogenetic group D and F was observed in E. coli isolates and several zoonotic sequence types (STs) including ST457 (14 isolates), ST38 (5), ST10 (2), ST117 (2) or ST93 (4) were detected. Isolates from three farms, which purchased chicken from a Paraguayan hatchery showed higher prevalence of mcr-5.1 and blaCTX-M-8 compared to the other nine farms, which purchased chickens from a Brazilian hatchery. Moreover, none of the K. pneumoniae isolates were linked to the Paraguayan hatchery. ESBL/AmpC and mcr-5-carrying multi-drug resistant (MDR) plasmids were characterized, and complete sequences were obtained for eight plasmids. The study shed light on Paraguayan poultry farms as a reservoir of antibiotic resistance commonly conferred via MDR plasmids and showed linkage between resistance and origin of the chickens at the hatcheries level. View Full-Text
Keywords: ESBL/AmpC; colistin; E. coli; K. pneumoniae; I1 plasmids ESBL/AmpC; colistin; E. coli; K. pneumoniae; I1 plasmids
Show Figures

Figure 1

MDPI and ACS Style

Nesporova, K.; Valcek, A.; Papagiannitsis, C.; Kutilova, I.; Jamborova, I.; Davidova-Gerzova, L.; Bitar, I.; Hrabak, J.; Literak, I.; Dolejska, M. Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries. Microorganisms 2021, 9, 866. https://doi.org/10.3390/microorganisms9040866

AMA Style

Nesporova K, Valcek A, Papagiannitsis C, Kutilova I, Jamborova I, Davidova-Gerzova L, Bitar I, Hrabak J, Literak I, Dolejska M. Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries. Microorganisms. 2021; 9(4):866. https://doi.org/10.3390/microorganisms9040866

Chicago/Turabian Style

Nesporova, Kristina, Adam Valcek, Costas Papagiannitsis, Iva Kutilova, Ivana Jamborova, Lenka Davidova-Gerzova, Ibrahim Bitar, Jaroslav Hrabak, Ivan Literak, and Monika Dolejska. 2021. "Multi-Drug Resistant Plasmids with ESBL/AmpC and mcr-5.1 in Paraguayan Poultry Farms: The Linkage of Antibiotic Resistance and Hatcheries" Microorganisms 9, no. 4: 866. https://doi.org/10.3390/microorganisms9040866

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop