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Microorganisms
Volume 9
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10.3390/microorganisms9010051
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Article

Comparison of Microbiological Characteristics and Genetic Diversity between Burkholderia cepacia Complex Isolates from Vascular Access and Other Clinical Infections

by
Min Yi Wong
1,2,3,
Yuan-Hsi Tseng
1,4,
Tsung-Yu Huang
2,4,5,
Bor-Shyh Lin
3,
Chun-Wu Tung
4,6,
Chishih Chu
7 and
Yao-Kuang Huang
1,4,5,*
1
Division of Thoracic and Cardiovascular Surgery, Chiayi Chang Gung Memorial Hospital, Chiayi County 61363, Taiwan
2
Microbiology Research and Treatment Center, Chiayi Chang Gung Memorial Hospital, Chiayi County 61363, Taiwan
3
Institute of Imaging and Biomedical Photonics, National Chiao Tung University, Tainan 71150, Taiwan
4
College of Medicine, Chang Gung University, Taoyuan City 33302, Taiwan
5
Division of Infectious Diseases, Department of Internal Medicine, Chiayi Chang Gung Memorial Hospital, Chiayi County 61363, Taiwan
6
Department of Nephrology, Chiayi Chang Gung Memorial Hospital, Chiayi County 61363, Taiwan
7
Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi City 60004, Taiwan
*
Author to whom correspondence should be addressed.
Microorganisms 2021, 9(1), 51; https://doi.org/10.3390/microorganisms9010051
Submission received: 12 November 2020 / Revised: 19 December 2020 / Accepted: 23 December 2020 / Published: 27 December 2020
(This article belongs to the Section Medical Microbiology)
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Abstract

Burkholderia cepacia complex (BCC) is a group of closely related bacteria with widespread environmental distribution. BCC bacteria are opportunistic pathogens that cause nosocomial infections in patients, especially cystic fibrosis (CF). Multilocus sequence typing (MLST) is used nowadays to differentiate species within the BCC complex. This study collected 41 BCC isolates from vascular access infections (VAIs) and other clinical infections between 2014 and 2020. We preliminarily identified bacterial isolates using standard biochemical procedures and further conducted recA gene sequencing and MLST for species identification. We determined genetic diversity indices using bioinformatics software. We studied 14 isolates retrieved from patients with VAIs and observed that Burkholderia cepacia was the predominant bacterial species, and B. contaminans followed by B. cenocepacia were mainly retrieved from patients with other infections. According to MLST data, we identified that all B. contaminans isolates belonged to ST102, while a wide variety of sequence types (STs) were found in B. cenocepacia isolates. In summary, the high diversity and easy transmission of BCC increase BCC infections, which provides insights into their potential clinical effects in non-CF infections.
Keywords: Burkholderia cepacia complex (BCC); B. contaminans; B. cenocepacia; non-CF; recA sequencing; multilocus sequence typing (MLST) Burkholderia cepacia complex (BCC); B. contaminans; B. cenocepacia; non-CF; recA sequencing; multilocus sequence typing (MLST)

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MDPI and ACS Style

Wong, M.Y.; Tseng, Y.-H.; Huang, T.-Y.; Lin, B.-S.; Tung, C.-W.; Chu, C.; Huang, Y.-K. Comparison of Microbiological Characteristics and Genetic Diversity between Burkholderia cepacia Complex Isolates from Vascular Access and Other Clinical Infections. Microorganisms 2021, 9, 51. https://doi.org/10.3390/microorganisms9010051

AMA Style

Wong MY, Tseng Y-H, Huang T-Y, Lin B-S, Tung C-W, Chu C, Huang Y-K. Comparison of Microbiological Characteristics and Genetic Diversity between Burkholderia cepacia Complex Isolates from Vascular Access and Other Clinical Infections. Microorganisms. 2021; 9(1):51. https://doi.org/10.3390/microorganisms9010051

Chicago/Turabian Style

Wong, Min Yi, Yuan-Hsi Tseng, Tsung-Yu Huang, Bor-Shyh Lin, Chun-Wu Tung, Chishih Chu, and Yao-Kuang Huang. 2021. "Comparison of Microbiological Characteristics and Genetic Diversity between Burkholderia cepacia Complex Isolates from Vascular Access and Other Clinical Infections" Microorganisms 9, no. 1: 51. https://doi.org/10.3390/microorganisms9010051

APA Style

Wong, M. Y., Tseng, Y.-H., Huang, T.-Y., Lin, B.-S., Tung, C.-W., Chu, C., & Huang, Y.-K. (2021). Comparison of Microbiological Characteristics and Genetic Diversity between Burkholderia cepacia Complex Isolates from Vascular Access and Other Clinical Infections. Microorganisms, 9(1), 51. https://doi.org/10.3390/microorganisms9010051

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