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Microorganisms
Volume 9
Issue 1
10.3390/microorganisms9010133
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Case Report

Hypervirulent Klebsiella pneumoniae of Lineage ST66-K2 Caused Tonsillopharyngitis in a German Patient

by
Kathleen Klaper
1,*,
Sebastian Wendt
2,3,
Christoph Lübbert
2,3,
Norman Lippmann
3,
Yvonne Pfeifer
1 and
Guido Werner
1
1
Division Nosocomial Pathogens and Antibiotic Resistance, Department of Infectious Diseases, Robert Koch Institute, Wernigerode Branch, 38855 Wernigerode, Germany
2
Division of Infectious Diseases and Tropical Medicine, Department of Medicine II, Leipzig University Hospital, 04109 Leipzig, Germany
3
Interdisciplinary Centre for Infectious Diseases, Leipzig University Hospital, 04109 Leipzig, Germany
*
Author to whom correspondence should be addressed.
Microorganisms 2021, 9(1), 133; https://doi.org/10.3390/microorganisms9010133
Submission received: 8 December 2020 / Revised: 5 January 2021 / Accepted: 6 January 2021 / Published: 8 January 2021
(This article belongs to the Special Issue Virulence and Resistance of Klebsiella pneumoniae)
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Abstract

Hypervirulent Klebsiella pneumoniae (hvKp) is a novel pathotype that has been rarely described in Europe. This study characterizes a hvKp isolate that caused a community-acquired infection. The hypermucoviscous Klebsiella pneumoniae (K. pneumoniae) strain 18-0005 was obtained from a German patient with tonsillopharyngitis in 2017. Antibiotic susceptibility testing was performed and the genome was sequenced by Illumina and Nanopore technology. Whole genome data were analyzed by conducting core genome multilocus sequence typing (cgMLST) and single nucleotide polymorphism (SNP) analysis. Virulence genes were predicted by applying Kleborate. Phenotypic and whole genome analyses revealed a high similarity of the study isolate 18-0005 to the recently reported antibiotic-susceptible hvKp isolate SB5881 from France and the “ancestral” strain Kp52.145; both were assigned to the ST66-K2 lineage. Comparative genomic analysis of the three plasmids showed that the 18-0005 plasmid II differs from SB5881 plasmid II by an additional 3 kb integrated fragment of plasmid I. Our findings demonstrate the genetic flexibility of hvKp and the occurrence of a strain of the clonal group CG66-K2 in Germany. Hence, it emphasizes the need to improve clinical awareness and infection monitoring of hvKp.
Keywords: Klebsiella pneumoniae; community-acquired infection; virulence; plasmids; Germany Klebsiella pneumoniae; community-acquired infection; virulence; plasmids; Germany

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MDPI and ACS Style

Klaper, K.; Wendt, S.; Lübbert, C.; Lippmann, N.; Pfeifer, Y.; Werner, G. Hypervirulent Klebsiella pneumoniae of Lineage ST66-K2 Caused Tonsillopharyngitis in a German Patient. Microorganisms 2021, 9, 133. https://doi.org/10.3390/microorganisms9010133

AMA Style

Klaper K, Wendt S, Lübbert C, Lippmann N, Pfeifer Y, Werner G. Hypervirulent Klebsiella pneumoniae of Lineage ST66-K2 Caused Tonsillopharyngitis in a German Patient. Microorganisms. 2021; 9(1):133. https://doi.org/10.3390/microorganisms9010133

Chicago/Turabian Style

Klaper, Kathleen, Sebastian Wendt, Christoph Lübbert, Norman Lippmann, Yvonne Pfeifer, and Guido Werner. 2021. "Hypervirulent Klebsiella pneumoniae of Lineage ST66-K2 Caused Tonsillopharyngitis in a German Patient" Microorganisms 9, no. 1: 133. https://doi.org/10.3390/microorganisms9010133

APA Style

Klaper, K., Wendt, S., Lübbert, C., Lippmann, N., Pfeifer, Y., & Werner, G. (2021). Hypervirulent Klebsiella pneumoniae of Lineage ST66-K2 Caused Tonsillopharyngitis in a German Patient. Microorganisms, 9(1), 133. https://doi.org/10.3390/microorganisms9010133

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