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Open AccessArticle

Characteristics of Carbapenem-Resistant and Colistin-Resistant Escherichia coli Co-Producing NDM-1 and MCR-1 from Pig Farms in China

by Zhong Peng 1,2,†, Xiaosong Li 1,2,†, Zizhe Hu 1,2, Zugang Li 1,2, Yujin Lv 2,3, Minggang Lei 2, Bin Wu 1,2, Huanchun Chen 1,2 and Xiangru Wang 1,2,*
1
State Key Laboratory of Agricultural Microbiology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430040, China
2
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430040, China
3
College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450044, China
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Microorganisms 2019, 7(11), 482; https://doi.org/10.3390/microorganisms7110482
Received: 19 September 2019 / Revised: 14 October 2019 / Accepted: 22 October 2019 / Published: 23 October 2019
(This article belongs to the Special Issue Antimicrobial Resistance in Livestock)
The emergence of carbapenem-resistant and colistin-resistant Enterobacteriaceae represents a great risk for public health. In this study, the phenotypical and genetic characteristics of eight carbapenem-resistant and colistin-resistant isolates from pig farms in China were determined by the broth microdilution method and whole genome sequencing. Antimicrobial susceptibility testing showed that the eight carbapenem-resistant and colistin-resistant strains were resistant to three aminoglycosides, twelve β-lactams, one of the phenicols, one of the tetracyclines, and one of the fluoroquinolones tested, simultaneously. The prediction of acquired resistant genes using the whole genome sequences revealed the co-existence of blaNDM-1 and mcr-1 as well as the other genes that were responsible for the multidrug-resistant phenotypes. Bioinformatics analysis also showed that the carbapenem-resistant gene blaNDM-1 was located on a putative IncFII-type plasmid, which also carried the other acquired resistant genes identified, including fosA3, blaTEM-1B and rmtB, while the colistin-resistant gene mcr-1 was carried by a putative IncX4-type plasmid. Finally, we found that these resistant genes/plasmids were conjugative, and they could be co-conjugated, conferring resistance to multiple types of antibiotics, including the carbapenems and colistin, to the recipient Escherichia coli strains. View Full-Text
Keywords: Escherichia coli; carbapenem-resistance; colistin-resistance; blaNDM-1; mcr-1; plasmid; co-existence Escherichia coli; carbapenem-resistance; colistin-resistance; blaNDM-1; mcr-1; plasmid; co-existence
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MDPI and ACS Style

Peng, Z.; Li, X.; Hu, Z.; Li, Z.; Lv, Y.; Lei, M.; Wu, B.; Chen, H.; Wang, X. Characteristics of Carbapenem-Resistant and Colistin-Resistant Escherichia coli Co-Producing NDM-1 and MCR-1 from Pig Farms in China. Microorganisms 2019, 7, 482.

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