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Article

Consolidated Bioprocess for Bioethanol Production from Raw Flour of Brosimum alicastrum Seeds Using the Native Strain of Trametes hirsuta Bm-2

1
Renewable Energy Department, Yucatan Center for Scientific Research, Merida 97302, Mexico
2
Natural Resource Department, Yucatan Center for Scientific Research, Merida 97205, Mexico
3
Mycology Laboratory, Biology Institute, National Autonomous University of Mexico, Mexico 04510, Mexico
4
Biorefinery Group, Food Research Department, Faculty of Chemistry Sciences, Autonomous University of Coahuila, Saltillo 25280, Mexico
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(11), 483; https://doi.org/10.3390/microorganisms7110483
Received: 27 August 2019 / Revised: 23 September 2019 / Accepted: 7 October 2019 / Published: 23 October 2019
(This article belongs to the Section Microbial Biotechnology)
Consolidated bioprocessing (CBP), which integrates biological pretreatment, enzyme production, saccharification, and fermentation, is a promising operational strategy for cost-effective ethanol production from biomass. In this study, the use of a native strain of Trametes hirsuta (Bm-2) was evaluated for bioethanol production from Brosimum alicastrum in a CBP. The raw seed flour obtained from the ramon tree contained 61% of starch, indicating its potential as a raw material for bioethanol production. Quantitative assays revealed that the Bm-2 strain produced the amylase enzyme with activity of 193.85 U/mL. The Bm-2 strain showed high tolerance to ethanol stress and was capable of directly producing ethanol from raw flour at a concentration of 13 g/L, with a production yield of 123.4 mL/kg flour. This study demonstrates the potential of T. hirsuta Bm-2 for starch-based ethanol production in a consolidated bioprocess to be implemented in the biofuel industry. The residual biomass after fermentation showed an average protein content of 22.5%, suggesting that it could also be considered as a valuable biorefinery co-product for animal feeding. View Full-Text
Keywords: consolidated bioprocess; biofuels; starch; α-amylase; white rot fungi consolidated bioprocess; biofuels; starch; α-amylase; white rot fungi
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MDPI and ACS Style

Olguin-Maciel, E.; Larqué-Saavedra, A.; Lappe-Oliveras, P.E.; Barahona-Pérez, L.F.; Alzate-Gaviria, L.; Chablé-Villacis, R.; Domínguez-Maldonado, J.; Pacheco-Catalán, D.; Ruíz, H.A.; Tapia-Tussell, R. Consolidated Bioprocess for Bioethanol Production from Raw Flour of Brosimum alicastrum Seeds Using the Native Strain of Trametes hirsuta Bm-2. Microorganisms 2019, 7, 483. https://doi.org/10.3390/microorganisms7110483

AMA Style

Olguin-Maciel E, Larqué-Saavedra A, Lappe-Oliveras PE, Barahona-Pérez LF, Alzate-Gaviria L, Chablé-Villacis R, Domínguez-Maldonado J, Pacheco-Catalán D, Ruíz HA, Tapia-Tussell R. Consolidated Bioprocess for Bioethanol Production from Raw Flour of Brosimum alicastrum Seeds Using the Native Strain of Trametes hirsuta Bm-2. Microorganisms. 2019; 7(11):483. https://doi.org/10.3390/microorganisms7110483

Chicago/Turabian Style

Olguin-Maciel, Edgar, Alfonso Larqué-Saavedra, Patricia E. Lappe-Oliveras, Luis F. Barahona-Pérez, Liliana Alzate-Gaviria, Rubí Chablé-Villacis, Jorge Domínguez-Maldonado, Daniella Pacheco-Catalán, Hector A. Ruíz, and Raúl Tapia-Tussell. 2019. "Consolidated Bioprocess for Bioethanol Production from Raw Flour of Brosimum alicastrum Seeds Using the Native Strain of Trametes hirsuta Bm-2" Microorganisms 7, no. 11: 483. https://doi.org/10.3390/microorganisms7110483

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