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Article

Phenotypic and Molecular Detection of Biofilm Formation in Staphylococcus aureus Isolated from Different Sources in Algeria

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Faculty of Nature and Life and Earth Sciences, Djilali-Bounaama University, Soufay, Khemis-Miliana 44225, Algeria
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Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany
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Faculty of Sciences, Yahia-Farès University, Urban Pole, 26000 Médéa, Algeria
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Leibniz Institute of Photonic Technology (IPHT), 07745 Jena, Germany
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InfectoGnostics Research Campus Jena e. V., 07743 Jena, Germany
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Institute for Physical Chemistry, Friedrich-Schiller-University, 07743 Jena, Germany
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Faculty of Veterinary Medicine, Kafrelsheik University, Kafr El-Sheik 35516, Egypt
*
Authors to whom correspondence should be addressed.
Pathogens 2020, 9(2), 153; https://doi.org/10.3390/pathogens9020153
Received: 5 February 2020 / Revised: 18 February 2020 / Accepted: 21 February 2020 / Published: 24 February 2020
(This article belongs to the Section Animal Pathogens)
Staphylococcus aureus is an opportunistic bacterium causing a wide variety of diseases. Biofilm formation of Staphylococcus aureus is of primary public and animal health concern. The purposes of the present study were to investigate the ability of Staphylococcus aureus isolated from animals, humans, and food samples to form biofilms and to screen for the presence of biofilm-associated and regulatory genes. In total, 55 Staphylococcus aureus isolated from sheep mastitis cases (n = 28), humans (n = 19), and from food matrices (n = 8) were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The ability of Staphylococcus aureus for slime production and biofilm formation was determined quantitatively. A DNA microarray examination was performed to detect adhesion genes (icaACD and biofilm-associated protein gene (bap)), genes encoding microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), regulatory genes (accessory gene regulator (agr) and staphylococcal accessory regulator (sarA)), and the staphylococcal cassette chromosome mec elements (SCCmec). Out of 55 Staphylococcus aureus isolates, 39 (71.0%) and 23 (41.8%) were producing slime and biofilm, respectively. All Staphylococcus aureus strains isolated from food showed biofilm formation ability. 52.6% of the Staphylococcus aureus strains isolated from sheep with mastitis, and 17.9% of isolates from humans, were able to form a biofilm. Microarray analysis typed the Staphylococcus aureus into 15 clonal complexes. Among all Staphylococcus aureus isolates, four of the human isolates (21.1%) harbored the mecA gene (SCCmec type IV) typed into 2 clonal complexes (CC22-MRSA-IV and CC80-MRSA-IV) and were considered as methicillin-resistant, while two of them were slime-producing. None of the isolates from sheep with mastitis harbored the cna gene which is associated with biofilm production. The fnbB gene was found in 100%, 60% and 40% of biofilm-producing Staphylococcus aureus isolated from food, humans, and sheep with mastitis, respectively. Three agr groups were present and agr group III was predominant with 43.6%, followed by agr group I (38.2%), and agr group II (18.2%). This study revealed the capacity of Staphylococcus aureus isolates to form biofilms and highlighted the genetic background displayed by Staphylococcus aureus isolates from different sources in Algeria. View Full-Text
Keywords: Staphylococcus aureus; biofilm formation; adhesion genes; microarray assay; Algeria Staphylococcus aureus; biofilm formation; adhesion genes; microarray assay; Algeria
MDPI and ACS Style

Achek, R.; Hotzel, H.; Nabi, I.; Kechida, S.; Mami, D.; Didouh, N.; Tomaso, H.; Neubauer, H.; Ehricht, R.; Monecke, S.; El-Adawy, H. Phenotypic and Molecular Detection of Biofilm Formation in Staphylococcus aureus Isolated from Different Sources in Algeria. Pathogens 2020, 9, 153. https://doi.org/10.3390/pathogens9020153

AMA Style

Achek R, Hotzel H, Nabi I, Kechida S, Mami D, Didouh N, Tomaso H, Neubauer H, Ehricht R, Monecke S, El-Adawy H. Phenotypic and Molecular Detection of Biofilm Formation in Staphylococcus aureus Isolated from Different Sources in Algeria. Pathogens. 2020; 9(2):153. https://doi.org/10.3390/pathogens9020153

Chicago/Turabian Style

Achek, Rachid, Helmut Hotzel, Ibrahim Nabi, Souad Kechida, Djamila Mami, Nassima Didouh, Herbert Tomaso, Heinrich Neubauer, Ralf Ehricht, Stefan Monecke, and Hosny El-Adawy. 2020. "Phenotypic and Molecular Detection of Biofilm Formation in Staphylococcus aureus Isolated from Different Sources in Algeria" Pathogens 9, no. 2: 153. https://doi.org/10.3390/pathogens9020153

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