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Transient Transfection of the Zoonotic Parasite Babesia microti

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Nanchang Center for Disease Control and Prevention, Nanchang 330038, China
Author to whom correspondence should be addressed.
Pathogens 2020, 9(2), 108;
Received: 7 January 2020 / Revised: 8 February 2020 / Accepted: 10 February 2020 / Published: 10 February 2020
(This article belongs to the Section Human Pathogens)
The development of genetic manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not been established for Babesia microti. Here, we report the first successful transient transfection of B. microti. The plasmids containing the firefly luciferase reporter gene were transfected into B. microti by an AMAXA 4D Nucleofection system. Twenty-four-hour synchronization, the 5′-actin promoter, program FA100, and 50 μg of plasmid DNA constituted the best conditions for the transient transfection of B. microti. This finding is the first step towards a stable transfection method for B. microti, which may contribute to a better understanding of the biology of the parasite. View Full-Text
Keywords: human babesiosis; Babesia microti; transient transfection human babesiosis; Babesia microti; transient transfection
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Liu, M.; Ji, S.; Rizk, M.A.; Adjou Moumouni, P.F.; Galon, E.M.; Li, J.; Li, Y.; Zheng, W.; Benedicto, B.; Tumwebaze, M.A.; Asada, M.; Xuan, X. Transient Transfection of the Zoonotic Parasite Babesia microti. Pathogens 2020, 9, 108.

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