Next Article in Journal
Vascular Leak and Hypercytokinemia Associated with Severe Fever with Thrombocytopenia Syndrome Virus Infection in Mice
Previous Article in Journal
New Taxon-Specific Heterobasidion PCR Primers Detect and Differentiate North American Heterobasidion spp. in Various Substrates and Led to the Discovery of Heterobasidion irregulare in British Columbia, Canada
Open AccessArticle

Evolutionary Insight into the Trypanosomatidae Using Alignment-Free Phylogenomics of the Kinetoplast

1
School of Life Sciences, University of Technology Sydney, Ultimo, NSW 2007, Australia
2
Department of Microbiology, St Vincent’s Hospital Sydney, Darlinghurst, NSW 2010, Australia
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(3), 157; https://doi.org/10.3390/pathogens8030157
Received: 23 August 2019 / Revised: 10 September 2019 / Accepted: 13 September 2019 / Published: 18 September 2019
(This article belongs to the Special Issue Kinetoplastid Phylogenomics and Evolution)
Advancements in next-generation sequencing techniques have led to a substantial increase in the genomic information available for analyses in evolutionary biology. As such, this data requires the exponential growth in bioinformatic methods and expertise required to understand such vast quantities of genomic data. Alignment-free phylogenomics offer an alternative approach for large-scale analyses that may have the potential to address these challenges. The evolutionary relationships between various species within the trypanosomatid family, specifically members belonging to the genera Leishmania and Trypanosoma have been extensively studies over the last 30 years. However, there is a need for a more exhaustive analysis of the Trypanosomatidae, summarising the evolutionary patterns amongst the entire family of these important protists. The mitochondrial DNA of the trypanosomatids, better known as the kinetoplast, represents a valuable taxonomic marker given its unique presence across all kinetoplastid protozoans. The aim of this study was to validate the reliability and robustness of alignment-free approaches for phylogenomic analyses and its applicability to reconstruct the evolutionary relationships between the trypanosomatid family. In the present study, alignment-free analyses demonstrated the strength of these methods, particularly when dealing with large datasets compared to the traditional phylogenetic approaches. We present a maxicircle genome phylogeny of 46 species spanning the trypanosomatid family, demonstrating the superiority of the maxicircle for the analysis and taxonomic resolution of the Trypanosomatidae. View Full-Text
Keywords: Trypanosomatidae; kinetoplast; second-generation sequencing; third-generation sequencing; alignment-free phylogenetics Trypanosomatidae; kinetoplast; second-generation sequencing; third-generation sequencing; alignment-free phylogenetics
Show Figures

Figure 1

MDPI and ACS Style

Kaufer, A.; Stark, D.; Ellis, J. Evolutionary Insight into the Trypanosomatidae Using Alignment-Free Phylogenomics of the Kinetoplast. Pathogens 2019, 8, 157.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop