Importance of B Lymphocytes and the IgG-Binding Protein Sbi in Staphylococcus aureus Skin Infection
1
Department of Pediatrics, The University of Chicago, Knapp Center for Biomedical Discovery 5124, 900 E. 57th Street, Chicago, IL 60637, USA
2
Department of Surgery, The University of Chicago, Chicago, IL 60637; USA
*
Author to whom correspondence should be addressed.
Academic Editor: Rachel McLoughlin
Pathogens 2016, 5(1), 12; https://doi.org/10.3390/pathogens5010012
Received: 1 September 2015
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Revised: 19 January 2016
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Accepted: 21 January 2016
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Published: 27 January 2016
(This article belongs to the Special Issue Staphylococcus Aureus Infection)
Recurrent Staphylococcus aureus infections are common, suggesting that immunity elicited by these infections is not protective. We previously reported that S. aureus skin infection (SSTI) elicited antibody-mediated immunity against secondary SSTI in BALB/c mice. In this study, we investigated the role of humoral immunity and the IgG-binding proteins Sbi and SpA in S. aureus SSTI. We found that B lymphocyte-deficient μMT mice were highly susceptible to infection, compared with congenic BALB/c mice. Importantly, transfer of immune serum protected μMT mice, demonstrating an appropriate response to protective antibody. We found that deletion of sbi, but not spa, impaired virulence, as assessed by skin lesion severity, and that Sbi-mediated virulence required B lymphocytes/antibody. Furthermore, neither Sbi nor SpA impaired the elicited antibody response or protection against secondary SSTI. Taken together, these findings highlight a B lymphocyte/antibody-dependent role of Sbi in the pathogenesis of S. aureus SSTI, and demonstrate that neither Sbi nor SpA interfered with elicited antibody-mediated immunity.
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Keywords:
MRSA; skin infection; Sbi; protein A; Staphylococcus aureus; antibody; protective immunity
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MDPI and ACS Style
Zhao, F.; Chong, A.S.; Montgomery, C.P. Importance of B Lymphocytes and the IgG-Binding Protein Sbi in Staphylococcus aureus Skin Infection. Pathogens 2016, 5, 12. https://doi.org/10.3390/pathogens5010012
AMA Style
Zhao F, Chong AS, Montgomery CP. Importance of B Lymphocytes and the IgG-Binding Protein Sbi in Staphylococcus aureus Skin Infection. Pathogens. 2016; 5(1):12. https://doi.org/10.3390/pathogens5010012
Chicago/Turabian StyleZhao, Fan, Anita S. Chong, and Christopher P. Montgomery. 2016. "Importance of B Lymphocytes and the IgG-Binding Protein Sbi in Staphylococcus aureus Skin Infection" Pathogens 5, no. 1: 12. https://doi.org/10.3390/pathogens5010012
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