In the original publication [
1], there was a mistake in
Table 2 as published. All oligonucleotides of PCR 1 in
Table 2 had erroneously been printed in reverse-complement style. The corrected
Table 2 appears below. The authors apologize for any inconvenience caused and state that the scientific conclusions are unaffected. The original publication has also been updated.
Table 2.
Details of the real-time PCR assays 1–6, which were included in the test comparison without a reference standard with perfect accuracy for the diagnosis of microsporidia in stool samples. Positive control plasmid inserts are provided in Appendix A.
Reference
- Tanida, K.; Hahn, A.; Eberhardt, K.A.; Tannich, E.; Landt, O.; Kann, S.; Feldt, T.; Sarfo, F.S.; Di Cristanziano, V.; Frickmann, H.; et al. Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples. Pathogens 2021, 10, 656. [Google Scholar] [CrossRef] [PubMed]
Table 2.
Details of the real-time PCR assays 1–6, which were included in the test comparison without a reference standard with perfect accuracy for the diagnosis of microsporidia in stool samples. Positive control plasmid inserts are provided in Appendix A.
| | PCR 1 | PCR 2 | PCR 3 | PCR 4 | PCR 5 | PCR 6 |
|---|
| Target specificity | Small subunit ribosomal RNA gene of Enterocytozoon bieneusi, Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis | Small subunit ribosomal RNA gene of Enterocytozoon bieneusi, Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis | Small subunit ribosomal RNA gene of Enterocytozoon bieneusi | Internal transcribed spacer (ITS) sequence of Enterocytozoon bieneusi | Small subunit ribosomal RNA gene of Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis | Internal transcribed spacer (ITS) sequence of the non-target microorganism Microsporidium spp. |
| Amplicon length | 394 base pairs | 280 base pairs | 202 base pairs | 105 base pairs | 227 base pairs | 87 base pairs |
| Cycle number | 50 | 40 | 40 | 50 | 40 | 45 |
| Forward primer 1 | 5′-CACCAGGTTGATTCTGCCTGA-3′ | 5′-CAGGTTGATTCTGCCTGACG-3′ | 5′-CCAGGGTCAAGTCATTCGTT-3′ | 5′-TGTGTAGGCGTGAGAGTGTATCTG-3′ | 5′-CACCAGGTTGATTCTGCCTGAC-3′ | 5′-TCTTGCGCGTTAATGATCCTT-3′ |
| Forward primer 2 | 5′-TCCGGAGAGGGAGCCTGAG-3′ | n.a. | n.a. | n.a. | n.a. | n.a. |
| Reverse primer 1 | 5′-GCTTGCCCTCCAATTGCTTC-3′ | 5′-CCATCTCTCAGGCTCCCTC-3′ | 5′-TATTGTATTGCGCTTGCTGC-3′ | 5′-CATCCAACCATCACGTACCAATC-3′ | 5′-CTAGTTAGGCCATTACCCTAACTACCA-3′ | 5′-AGGTTGCGGGCGGC-3′ |
| Reverse primer 2 | 5′-GACTTGCCCTCCAATCACATG-3′ | n.a. | n.a. | n.a. | n.a. | n.a. |
| Reverse primer 3 | 5′-CCGACTTGCCCTCCAGTAAA-3′ | n.a. | n.a. | n.a. | n.a. | n.a. |
| Reverse primer 4 | 5′-CTTGGCCTCCAATCAATCTCG-3′ | n.a. | n.a. | n.a. | n.a. | n.a. |
| Hybridization probe * | 5′-TGGCAGCAGGCGCGAAACTTGT-3′ | n.a. | 5′-GATGCCCTTAGATATCCTGG-3′ | 5′-CACTGCACCCACATCCCTCACCCTT-3′ | 5′-CTATCACTGAG+C+CGT+CC-3′ | 5′-ACGGAAGAGCTTCGGGGGCCA-3′ |
| Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
