Correction: Tanida et al. Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples. Pathogens 2021, 10, 656

In the original publication [1], there was a mistake in

Table 2. Details of the real-time PCR assays 1–6, which were included in the test comparison without a reference standard with perfect accuracy for the diagnosis of microsporidia in stool samples. Positive control plasmid inserts are provided in Appendix A.

	PCR 1	PCR 2	PCR 3	PCR 4	PCR 5	PCR 6
Target specificity	Small subunit ribosomal RNA gene of Enterocytozoon bieneusi, Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis	Small subunit ribosomal RNA gene of Enterocytozoon bieneusi, Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis	Small subunit ribosomal RNA gene of Enterocytozoon bieneusi	Internal transcribed spacer (ITS) sequence of Enterocytozoon bieneusi	Small subunit ribosomal RNA gene of Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis	Internal transcribed spacer (ITS) sequence of the non-target microorganism Microsporidium spp.
Amplicon length	394 base pairs	280 base pairs	202 base pairs	105 base pairs	227 base pairs	87 base pairs
Cycle number	50	40	40	50	40	45
Forward primer 1	5′-CACCAGGTTGATTCTGCCTGA-3′	5′-CAGGTTGATTCTGCCTGACG-3′	5′-CCAGGGTCAAGTCATTCGTT-3′	5′-TGTGTAGGCGTGAGAGTGTATCTG-3′	5′-CACCAGGTTGATTCTGCCTGAC-3′	5′-TCTTGCGCGTTAATGATCCTT-3′
Forward primer 2	5′-TCCGGAGAGGGAGCCTGAG-3′	n.a.	n.a.	n.a.	n.a.	n.a.
Reverse primer 1	5′-GCTTGCCCTCCAATTGCTTC-3′	5′-CCATCTCTCAGGCTCCCTC-3′	5′-TATTGTATTGCGCTTGCTGC-3′	5′-CATCCAACCATCACGTACCAATC-3′	5′-CTAGTTAGGCCATTACCCTAACTACCA-3′	5′-AGGTTGCGGGCGGC-3′
Reverse primer 2	5′-GACTTGCCCTCCAATCACATG-3′	n.a.	n.a.	n.a.	n.a.	n.a.
Reverse primer 3	5′-CCGACTTGCCCTCCAGTAAA-3′	n.a.	n.a.	n.a.	n.a.	n.a.
Reverse primer 4	5′-CTTGGCCTCCAATCAATCTCG-3′	n.a.	n.a.	n.a.	n.a.	n.a.
Hybridization probe *	5′-TGGCAGCAGGCGCGAAACTTGT-3′	n.a.	5′-GATGCCCTTAGATATCCTGG-3′	5′-CACTGCACCCACATCCCTCACCCTT-3′	5′-CTATCACTGAG+C+CGT+CC-3′	5′-ACGGAAGAGCTTCGGGGGCCA-3′

n.a. = not applicable. * Bases with a plus (+) in front of them are locked nucleic acid (LNA) bases.

as published. All oligonucleotides of PCR 1 in Table 2 had erroneously been printed in reverse-complement style. The corrected Table 2 appears below. The authors apologize for any inconvenience caused and state that the scientific conclusions are unaffected. The original publication has also been updated.