Correction: Tanida et al. Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples. Pathogens 2021, 10, 656
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1
Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, Germany
2
Department of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany
3
Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine & I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany
4
Institute for Transfusion Medicine, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany
In the original publication [1], there was a mistake in Table 2 as published. All oligonucleotides of PCR 1 in Table 2 had erroneously been printed in reverse-complement style. The corrected Table 2 appears below. The authors apologize for any inconvenience caused and state that the scientific conclusions are unaffected. The original publication has also been updated.
Table 2.
Details of the real-time PCR assays 1–6, which were included in the test comparison without a reference standard with perfect accuracy for the diagnosis of microsporidia in stool samples. Positive control plasmid inserts are provided in Appendix A.
Reference
Tanida, K.; Hahn, A.; Eberhardt, K.A.; Tannich, E.; Landt, O.; Kann, S.; Feldt, T.; Sarfo, F.S.; Di Cristanziano, V.; Frickmann, H.; et al. Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples. Pathogens2021, 10, 656. [Google Scholar] [CrossRef] [Scilit] [PubMed]
Table 2.
Details of the real-time PCR assays 1–6, which were included in the test comparison without a reference standard with perfect accuracy for the diagnosis of microsporidia in stool samples. Positive control plasmid inserts are provided in Appendix A.
Table 2.
Details of the real-time PCR assays 1–6, which were included in the test comparison without a reference standard with perfect accuracy for the diagnosis of microsporidia in stool samples. Positive control plasmid inserts are provided in Appendix A.
PCR 1
PCR 2
PCR 3
PCR 4
PCR 5
PCR 6
Target specificity
Small subunit ribosomal RNA gene of Enterocytozoon bieneusi, Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis
Small subunit ribosomal RNA gene of Enterocytozoon bieneusi, Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis
Small subunit ribosomal RNA gene of Enterocytozoon bieneusi
Internal transcribed spacer (ITS) sequence of Enterocytozoon bieneusi
Small subunit ribosomal RNA gene of Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis
Internal transcribed spacer (ITS) sequence of the non-target microorganism Microsporidium spp.
Amplicon length
394 base pairs
280 base pairs
202 base pairs
105 base pairs
227 base pairs
87 base pairs
Cycle number
50
40
40
50
40
45
Forward primer 1
5′-CACCAGGTTGATTCTGCCTGA-3′
5′-CAGGTTGATTCTGCCTGACG-3′
5′-CCAGGGTCAAGTCATTCGTT-3′
5′-TGTGTAGGCGTGAGAGTGTATCTG-3′
5′-CACCAGGTTGATTCTGCCTGAC-3′
5′-TCTTGCGCGTTAATGATCCTT-3′
Forward primer 2
5′-TCCGGAGAGGGAGCCTGAG-3′
n.a.
n.a.
n.a.
n.a.
n.a.
Reverse primer 1
5′-GCTTGCCCTCCAATTGCTTC-3′
5′-CCATCTCTCAGGCTCCCTC-3′
5′-TATTGTATTGCGCTTGCTGC-3′
5′-CATCCAACCATCACGTACCAATC-3′
5′-CTAGTTAGGCCATTACCCTAACTACCA-3′
5′-AGGTTGCGGGCGGC-3′
Reverse primer 2
5′-GACTTGCCCTCCAATCACATG-3′
n.a.
n.a.
n.a.
n.a.
n.a.
Reverse primer 3
5′-CCGACTTGCCCTCCAGTAAA-3′
n.a.
n.a.
n.a.
n.a.
n.a.
Reverse primer 4
5′-CTTGGCCTCCAATCAATCTCG-3′
n.a.
n.a.
n.a.
n.a.
n.a.
Hybridization probe *
5′-TGGCAGCAGGCGCGAAACTTGT-3′
n.a.
5′-GATGCCCTTAGATATCCTGG-3′
5′-CACTGCACCCACATCCCTCACCCTT-3′
5′-CTATCACTGAG+C+CGT+CC-3′
5′-ACGGAAGAGCTTCGGGGGCCA-3′
n.a. = not applicable. * Bases with a plus (+) in front of them are locked nucleic acid (LNA) bases.
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