First Report of Genetic Variability of Erysipelothrix sp. Strain 2 in Turkeys Associated to Vero Cells Morphometric Alteration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples
2.2. Identification of Erysipelothrix spp.
2.3. Transmission Electronic Microscopy (TEM)
2.4. Pulsed Field Gel Electrophoresis (PFGE)
2.5. Epidemiological and Statistical Analysis
2.6. Cellular Morphometry
2.7. Apoptosis
2.8. Invasion Test
2.9. Statistical Analysis
3. Results
3.1. Electron Microscopy of ES2
3.2. Epidemiologic Analysis Relative to PFGE
3.3. Morphometry in Vero Cells
3.4. Apoptosis
3.5. Bacterial Multiplication
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Est06 | Est07 | LB | |
---|---|---|---|
Initial inoculum | 3.00 a | 3.00 a | 3.00 |
Inside the cell culture medium | 7.22 b A | 7.11 b A | 0 |
After four washes | 2.89 a B | 2.81 a B | 0 |
After treatment with triton | 3.13 a C | 3.23 a C | 0 |
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dos Reis, T.F.M.; Hoepers, P.G.; Peres, P.A.B.M.; Mendonça, E.P.; de Sousa Braga, P.F.; Beletti, M.E.; Rossi, D.A.; Grazziotin, A.L.; Goulart, L.R.; Fonseca, B.B. First Report of Genetic Variability of Erysipelothrix sp. Strain 2 in Turkeys Associated to Vero Cells Morphometric Alteration. Pathogens 2021, 10, 141. https://doi.org/10.3390/pathogens10020141
dos Reis TFM, Hoepers PG, Peres PABM, Mendonça EP, de Sousa Braga PF, Beletti ME, Rossi DA, Grazziotin AL, Goulart LR, Fonseca BB. First Report of Genetic Variability of Erysipelothrix sp. Strain 2 in Turkeys Associated to Vero Cells Morphometric Alteration. Pathogens. 2021; 10(2):141. https://doi.org/10.3390/pathogens10020141
Chicago/Turabian Styledos Reis, Thais Fernanda Martins, Patrícia Giovana Hoepers, Phelipe Augusto Borba Martins Peres, Eliane Pereira Mendonça, Paula Fernanda de Sousa Braga, Marcelo Emilio Beletti, Daise Aparecida Rossi, Ana Laura Grazziotin, Luiz Ricardo Goulart, and Belchiolina Beatriz Fonseca. 2021. "First Report of Genetic Variability of Erysipelothrix sp. Strain 2 in Turkeys Associated to Vero Cells Morphometric Alteration" Pathogens 10, no. 2: 141. https://doi.org/10.3390/pathogens10020141
APA Styledos Reis, T. F. M., Hoepers, P. G., Peres, P. A. B. M., Mendonça, E. P., de Sousa Braga, P. F., Beletti, M. E., Rossi, D. A., Grazziotin, A. L., Goulart, L. R., & Fonseca, B. B. (2021). First Report of Genetic Variability of Erysipelothrix sp. Strain 2 in Turkeys Associated to Vero Cells Morphometric Alteration. Pathogens, 10(2), 141. https://doi.org/10.3390/pathogens10020141