Deletion of the Serotonin Receptor 7 Gene Changed the Development and Behavior of the Mosquito, Aedes aegypti
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insects
2.2. Phylogenetic Analysis
2.3. qPCR
2.4. CRISPR-Cas9-Mediated Knockout of the Ae. aegypti 5-HTR7A
2.5. Evaluation of Stress Response the Mutants
2.6. Statistical Analysis
3. Results
3.1. Phylogenetic Analysis of Ae. aegypti 5-HTR Proteins
3.2. Temporal and Spatial Expression Profiles of 5-HTR7A Genes
3.3. CRISPR-Cas9-Mediated 5-HTR7A Gene Knockout
3.4. Morphological Changes in 5-HTR7A Mutant Mosquitoes
3.5. Stress Response of 5-HTR7A Mutant Mosquitoes
3.6. Effects of 5-HTR7A Mutations on Egg Hatching in Offspring
3.7. 5-HTR7A Expression in Mutant Mosquitoes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Li, M.; Zhang, L.; Wu, Y.; Li, Y.; Chen, X.; Chen, J.; Wang, Q.; Liao, C.; Han, Q. Deletion of the Serotonin Receptor 7 Gene Changed the Development and Behavior of the Mosquito, Aedes aegypti. Insects 2022, 13, 671. https://doi.org/10.3390/insects13080671
Li M, Zhang L, Wu Y, Li Y, Chen X, Chen J, Wang Q, Liao C, Han Q. Deletion of the Serotonin Receptor 7 Gene Changed the Development and Behavior of the Mosquito, Aedes aegypti. Insects. 2022; 13(8):671. https://doi.org/10.3390/insects13080671
Chicago/Turabian StyleLi, Miaozhen, Lei Zhang, Yuchen Wu, Yixun Li, Xin Chen, Jing Chen, Qiuhui Wang, Chenghong Liao, and Qian Han. 2022. "Deletion of the Serotonin Receptor 7 Gene Changed the Development and Behavior of the Mosquito, Aedes aegypti" Insects 13, no. 8: 671. https://doi.org/10.3390/insects13080671