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Reduced Glutamine Synthetase Activity Alters the Fecundity of Female Bactrocera dorsalis (Hendel)

1
Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China
2
International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
3
Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent 9000, Belgium
*
Author to whom correspondence should be addressed.
Insects 2019, 10(7), 186; https://doi.org/10.3390/insects10070186
Received: 4 May 2019 / Revised: 24 June 2019 / Accepted: 25 June 2019 / Published: 27 June 2019
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Abstract

Glutamine synthetase (GS) is a key enzyme in glutamine synthesis and is associated with multiple physiological processes in insects, such as embryonic development, heat shock response, and fecundity regulation. However, little is known about the influence of GS on female fecundity in the oriental fruit fly, Bactrocera dorsalis. Based on the cloning of BdGSs, mitochondrial BdGSm and cytoplasmic BdGSc, we determined their expressions in the tissues of adult B. dorsalis. BdGSm was highly expressed in the fat body, while BdGSc was highly expressed in the head and midgut. Gene silencing by RNA interference against two BdGSs isoforms suppressed target gene expression at the transcriptional level, leading to a reduced ovarian size and lower egg production. The specific inhibitor L-methionine S-sulfoximine suppressed enzyme activity, but only the gene expression of BdGSm was suppressed. A similar phenotype of delayed ovarian development occurred in the inhibitor bioassay. Significantly lower expression of vitellogenin and vitellogenin receptor was observed when GS enzyme activity was suppressed. These data illustrate the effects of two GS genes on adult fecundity by regulating vitellogenin synthesis in different ways. View Full-Text
Keywords: oriental fruit fly; glutamine synthetase; fecundity; L-methionine S-sulfoximine; vitellogenin oriental fruit fly; glutamine synthetase; fecundity; L-methionine S-sulfoximine; vitellogenin
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Wei, D.; Zhang, M.-Y.; Zhang, Y.-X.; Zhang, S.-Y.; Smagghe, G.; Wang, J.-J. Reduced Glutamine Synthetase Activity Alters the Fecundity of Female Bactrocera dorsalis (Hendel). Insects 2019, 10, 186.

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