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J. Pers. Med. 2019, 9(1), 2; https://doi.org/10.3390/jpm9010002

Association of TLR4 Polymorphisms, Expression, and Vitamin D with Helicobacter pylori Infection

1
Department of Life and Earth Sciences, Faculty of Sciences II, Lebanese University, Fanar P.O. Box 26110217, Lebanon
2
Faculty of Medicine, University of Ottawa, Ottawa, ON K1G 5Z3, Canada
3
Rammal Hassan Rammal Research Laboratory, Physio-toxicity (PhyTox) research group, Lebanese University, Faculty of Sciences (V), Nabatieh, Lebanon
4
Department of Biological and Environmental Sciences, Faculty of Science, Beirut Arab University, Lebanon
5
Université de Lorraine, Inserm, IGE-PCV, F-54000 Nancy, France
6
Department of Medical Laboratory Technology, Faculty of Health Sciences, Beirut Arab University, Beirut 1107 2809, Lebanon
*
Author to whom correspondence should be addressed.
These authors are contributed equally to this work.
Received: 15 November 2018 / Revised: 21 December 2018 / Accepted: 3 January 2019 / Published: 11 January 2019
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Abstract

Helicobacter pylori (H. pylori) infection is the strongest recognized risk factor for gastric adenocarcinoma. Since previous observations have shown that polymorphisms in innate immune system genes, as well as vitamin D (VitD) levels, could modify the risk of infection with Helicobacter pylori (H. pylori), we analyzed the relation between single nucleotide polymorphisms (SNPs) in TLRs (TLR1, TLR2, TLR4) CD14, RUNX3 and VitD levels with H. pylori infection. A case-control study on four hundred sixty Lebanese individuals was conducted. Eleven SNPs in total were genotyped and gene expression analysis using real-time PCR was performed in white blood cells of a subsample of eight individuals. A total of 49% of the participants were affected. Although no direct association was found between the SNPs and H. pylori infection, rs4986790G>A and rs4986791T>C in TLR4 were negatively associated with VitD levels (β = −0.371, p = 5 × 10−3 and β = −0.4, p = 2 × 10−3, respectively), which was negatively associated with H. pylori infection (OR = 0.01, p < 1 × 10−3). TLR4 expression was 3× lower in individuals with H. pylori compared with non-infected (p = 0.01). TLR4 polymorphisms, expression, and VitD could be implicated in H. pylori infection and further development of gastric adenocarcinoma. View Full-Text
Keywords: Helicobacter pylori; Toll-like Receptor 4; single nucleotide polymorphisms; gene expression; vitamin D Helicobacter pylori; Toll-like Receptor 4; single nucleotide polymorphisms; gene expression; vitamin D
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Assaad, S.; Costanian, C.; Jaffal, L.; Tannous, F.; Stathopoulou, M.G.; Shamieh, S.E. Association of TLR4 Polymorphisms, Expression, and Vitamin D with Helicobacter pylori Infection. J. Pers. Med. 2019, 9, 2.

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