Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes
AbstractPancreatic islets (PIs) transplantation is an alternative approach for the treatment of severe forms of type 1 diabetes (T1D). To monitor the success of transplantation, it is desirable to follow the location of engrafted PIs non-invasively. In vivo magnetic resonance imaging (MRI) of transplanted PIs is a feasible cell tracking method; however, this requires labeling with a suitable contrast agent prior to transplantation. We have tested the feasibility of cationic magnetoliposomes (MLs), compared to commercial contrast agents (Endorem and Resovist), by labeling insulinoma cells and freshly isolated rat PIs. It was possible to incorporate Magnetic Ressonance (MR)-detectable amounts of MLs in a shorter time (4 h) when compared to Endorem and Resovist. MLs did not show negative effects on the PIs’ viability and functional parameters in vitro. Labeled islets were transplanted in the renal sub-capsular region of healthy mice. Hypointense contrast in MR images due to the labeled PIs was detected in vivo upon transplantation, while MR detection of PIs labeled with Endorem and Resovist was only possible after the addition of transfection agents. These findings indicate that MLs are suitable to image PIs, without affecting their function, which is promising for future longitudinal pre-clinical and clinical studies involving the assessment of PI transplantation. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 367 KB)
Share & Cite This Article
Garcia Ribeiro, R.S.; Ketkar-Atre, A.; Yin, T.; Louchami, K.; Struys, T.; Lambrichts, I.; Sener, A.; Malaisse, W.J.; De Cuyper, M.; Himmelreich, U. Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes. J. Pers. Med. 2018, 8, 12.
Garcia Ribeiro RS, Ketkar-Atre A, Yin T, Louchami K, Struys T, Lambrichts I, Sener A, Malaisse WJ, De Cuyper M, Himmelreich U. Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes. Journal of Personalized Medicine. 2018; 8(1):12.Chicago/Turabian Style
Garcia Ribeiro, Rita S.; Ketkar-Atre, Ashwini; Yin, Ting; Louchami, Karim; Struys, Tom; Lambrichts, Ivo; Sener, Abdullah; Malaisse, Willy J.; De Cuyper, Marcel; Himmelreich, Uwe. 2018. "Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes." J. Pers. Med. 8, no. 1: 12.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.