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Article

PD-L1, CD4+, and CD8+ Tumor-Infiltrating Lymphocytes (TILs) Expression Profiles in Melanoma Tumor Microenvironment Cells

by
Bogdan Marian Caraban
1,
Elena Matei
2,*,
Georgeta Camelia Cozaru
2,3,
Mariana Aşchie
2,3,
Mariana Deacu
1,3,
Manuela Enciu
1,3,
Gabriela Izabela Bălţătescu
2,3,
Anca Chisoi
2,3,
Nicolae Dobrin
3,
Lucian Petcu
4,
Emma Gheorghe
1,
Laurențiu-Tony Hangan
1,
Mihai Cătălin Roșu
2,
Cristian Ionuț Orasanu
2,3 and
Antonela-Anca Nicolau
2,3
1
Faculty of Medicine, “Ovidius” University of Constanta, 900470 Constanta, Romania
2
Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, “Ovidius” University of Constanta, 900591 Constanta, Romania
3
Clinical Service of Pathology, “Sf. Apostol Andrei” Emergency County Hospital, 900591 Constanta, Romania
4
Dental Medicine Faculty, “Ovidius” University of Constanta, 900470 Constanta, Romania
*
Author to whom correspondence should be addressed.
J. Pers. Med. 2023, 13(2), 221; https://doi.org/10.3390/jpm13020221
Submission received: 20 December 2022 / Revised: 21 January 2023 / Accepted: 25 January 2023 / Published: 27 January 2023
(This article belongs to the Special Issue Skin Cancers: Biomarkers and Potential Therapeutic Targets)
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Abstract

(1) Background: Because melanoma is an aggressive tumor with an unfavorable prognosis, we aimed to characterize the PD-L1 expression in melanomas in association with T cell infiltrates because PD-1/PD-L1 blockade represents the target in treating melanoma strategy. (2) Methods: The immunohistochemical manual quantitative methods of PD-L1, CD4, and CD8 TILs were performed in melanoma tumor microenvironment cells. (3) Results: Most of the PD-L1 positive, expressing tumors, have a moderate score of CD4+ TILs and CD8+TILs (5−50% of tumor area) in tumoral melanoma environment cells. The PD-L1 expression in TILs was correlated with different degrees of lymphocytic infiltration described by the Clark system (X2 = 8.383, p = 0.020). PD-L1 expression was observed often in melanoma cases, with more than 2−4 mm of Breslow tumor thickness being the associated parameters (X2 = 9.933, p = 0.014). (4) Conclusions: PD-L1 expression represents a predictive biomarker with very good accuracy for discriminating the presence or absence of malign tumoral melanoma cells. PD-L1 expression was an independent predictor of good prognosis in patients with melanomas.

1. Introduction

Melanoma is a highly aggressive tumor, most often with a cutaneous presentation with an immunogenic character, unfavorable prognosis, and raised mortality rate. Older fair-skinned males represent the high-risk population with a personal or family history of melanoma and with chronic UV exposure [1,2]. In recent years, significant progress in targeted therapy development [3,4] and novel prognostic biomarkers have been necessary for patient treatment strategy tailoring [5].
It is recognized that the immune inhibitory signaling pathways have an important role in immunosuppressive microenvironment maintenance, which favor cancer development [6]. An important co-inhibitory pathway is the programmed death-ligand 1 (PD-L1) and programmed death-1 (PD-1) axis [7]. The expression of PD-1 is induced on effector T-cells in response to inflammatory signals, and PD-L1 (B7-H1 or CD274) was the first identified ligand of PD-1 and is expressed in lymphocytes, vascular endothelium, mesenchymal stem cells, neuronal cells, and tumor cells [8]. PD-1/PD-L1 interactions have roles in T-cell-mediated immune responses inhibiting, cytokine production limiting, and tumor immune escape promotion [5,9]. PD-L1 binding to PD-1 suppresses the immune response through CD8+ T cell inhibition and CD4+T-regulatory lymphocyte activation, and melanoma tumor cells use this mechanism [10,11]. PD-L1 is expressed in the tumor microenvironment cells facilitating immune evasion, a predictive biomarker for malignant melanoma evolution [12,13,14]. PD-L1 expression in tumor cells is the most studied predictive biomarker, but a correlation with a therapeutic response is insufficient for melanoma for clinical use. PD-L1 overexpression is examined as a prognostic factor in diverse cancers, such as lung cancer [15], gastric cancer [16], ovarian cancer [17], breast cancer [18], prostate cancer [19], bladder cancer [20], cervical cancer [21], colorectal cancer [22], pancreatic cancer [23], and renal cell carcinoma [24]. Various studies reported the prognostic value of PD-L1 expression in melanoma patients [5,25,26,27,28,29,30,31,32,33,34,35,36,37].
Other predictive biomarkers studied in melanoma tumors include T cell receptors and T cell infiltrates. CD8+ T cell presence in the melanoma periphery is associated with a better response to PD-1 inhibitors [38,39]. Most malignant melanocytic tumors evolved despite immune cell presence in the tumor microenvironment, suggesting that these lymphocytes fail to control tumor growth [7,40,41]. The tumor-infiltrating lymphocytes (TILs) were a part of many studies in the past few years [42], bringing important evidence that a high number of lymphocytes are present in the development site of a tumor, with numerous CD8-positive T lymphocytes. These lymphocytes present in melanoma must be differentiated from lymphocytes in a skin lymphoma that has immunoreactivity for CD20 but a negative reaction for CD30 and CD45 [43]. A statistically significant correlation between skin melanoma survivors and increased risk of Non-Hodgkin Lymphoma was observed in the first three years after the diagnosis of skin melanoma [44].
In this study, we used a melanoma patient cohort to characterize the PD-L1 expression in melanoma cases associated with T-cell infiltrates, with roles in diagnosis and prognosis.

2. Materials and Methods

2.1. Patient Group

This study collected the cases from the electronic database of the Pathology Department of ”St. Andrew’s” Clinical Emergency County Hospital of Constanta diagnosed with different forms of melanoma in the last two years. We identified 61 patients with surgically removed skin melanocytic tumors upon dermatological recommendation that proved to be melanomas before any oncological treatment. The histologic diagnosis for each specimen was confirmed on Hematoxylin Eosin (HE) stain slides by two experienced pathologists, with a concordance of measurements between 80−90%. We kept a representative tissue block to use for additional studies. Clinico-morphologic features were taken from the pathological reports, including age, gender, Clark level, Brelow thickness, TILs, and vascular and perineural invasion.
Our study followed these criteria: (1) inclusion of patients diagnosed with histologically confirmed melanoma; (2) detection of PD-L1, CD4, and CD8 TILs immunohistochemical expression in the melanomas (IHC) analysis; (3) identification of predictive biomarkers as PD-L1, CD4, CD8 tumor-infiltrating lymphocytes expression patterns; and calculation of the hazard ratio (HR) and 95% confidence interval (95% CI).

2.2. TILs Evaluation

TILs evaluation was performed on Hematoxylin Eosin (HE) stain using the Clark scoring system, classifying immune infiltrate as brisk (TILs present throughout the vertical growth), non-brisk (TILs in one or more foci of the vertical growth phase), or absent.

2.3. Immunohistochemical (IHC) Analysis

The immunohistochemical evaluation was performed with a panel of three antibodies from Master Diagnostica (Granada, Spain) (ready-to-use): PD-L1 (CAL10-clone, rabbit monoclonal antibody), CD4 (EP204-clone, rabbit monoclonal antibody), CD8 (SP16-clone, rabbit monoclonal antibody). We used formalin-fixed paraffin-embedded blocks to obtain four µm-thick sections, 3, 3′diaminobenzidine (DAB) as chromogen, with brown staining. The sections were finally counterstained with Mayer‘s Hematoxylin and mounted. For positive and negative quality controls, we used tonsil tissue according to the manufacturer’s recommendation. The three immunohistochemical biomarkers were evaluated using optic microscopy, considering the positive reaction ≥5%. The degrees of intensities were considered: weakly positive (1+), moderate positive (2+), and intense positive (3+). We assessed the PD-L1 expression in melanoma cells, and TIL’s and CD4 and CD8 TIL’s expression in melanoma tumor microenvironment cells.

2.4. Statistical Analysis

The experimental data were analyzed using IBM SPSS Statistics Version 26 (Armonk, NY: IBM Corp). The procedures used were descriptive statistics (for characterization of the discreet and continuous variables defined in the database), parametric test (Independent Sample t Test with Levene’s Test and One Way ANOVA with posthoc Tukey’s range test and Games-Howell Test), Nonparametric tests (Pearson Chi-Square with Cramer’s V test for the association between categorical variables, Mann-Whitney U Test and Kruskal-Wallis H Test for continuous variables). All p-values below 0.05 were considered statistically significant. Receiver operating characteristic (ROC) and area under the curve (AUC) made by MedCalc v20.111 Software Ltd. (Ostend, Belgium) were used to establish the accuracy of the biomarkers in melanoma diagnoses. The sensitivity and specificity of biomarkers are represented by the Youden index, which is the optimal cut-off point as the value that maximizes the area under the ROC curve [45,46]. Furthermore, multivariate Cox-proportional hazard regression was performed to determine the potential prognostic values of PD-L1, CD4, and CD8 TILs in melanoma patients.

3. Results

3.1. Clinico-Pathological Analysis of Melanoma Tumor Microenvironment Cells, Associated with IHC Patterns of PD-L1 (+/-) Expression

PD-L1 (+/-) expression patterns in patients with acral, cervical-cranial, and limb localization of melanomas are summarized in Table 1. The incidence of PDL-1 positive expressions for patients with acral and cervico-cranial localization of melanoma was notably lower than for patients with upper body and limbs localizations of melanoma (TILs-A:5.41%; CC:8.11% vs. UB:51.35%; L:35.14%, p < 0.05; MTCs-A:7.10%; CC:17.86% vs. UB:53.62%; L:21.43%, p ≥ 0.05). We observed PD-L1 positive expression patterns in 56.76% of TILs and 46.43% in MTCs (p ≥ 0.05) for IV grade of Clark level of invasion in melanoma samples from 61 patients when a TILs threshold of positive reaction ≥5%.
When comparing the Breslow tumor thickness between TILs with MTCs, were observed the lowest proportion of PD-L1 (+) expression, at 5.41% (TILs) and 0% (MTCs) for <1 mm diameter, while the highest proportion of PD-L1 (+) specimens was seen for >4mm diameter, at 54.05% (TILs, p ≥ 0.05) and 57.14% (MTCs, p < 0.05) from cases.
The association between PD-L1 expression and clinicopathological features was analyzed, age (≥65 vs. <65 years), gender (male vs. female), localization (acral, upper body, cervico-cranial, limb), Clark level of invasion (I, II, III, IV, V), Breslow tumor thickness (<1 mm; 1–2 mm; 2–4 mm; >4 mm), lymphovascular invasion (present, absent), perineural invasion (present, absent) (Table 1).
PD-L1 expression was significantly correlated with melanoma localizations in infiltrating immune cells (X2 = 9.603, p = 0.018) and Breslow tumor thickness in melanoma tumor cells (X2 = 9.933, p = 0.014), but no significant relationship between PD-L1 expression and age, gender, or morphological characteristics was seen (Clark level of invasion, lymphovascular invasion, and perineural invasion).

3.2. PD-L1 Expression in Tumor Cells and Immune-Infiltrating Cells in Various Morphological Types of Melanoma and Tils Quantification and Characterization

PD-L1 (+/-) expression patterns were observed in the different morphological types of melanomas as lentigo maligna melanoma (LMM), superficial spreading melanoma (SSM), superficial spreading melanoma with vertical growth nodule (SSM-VGN), and nodular melanoma (NM), being presented in Table 2.
The highest incidences of PD-L1 (+) in TILs were observed for SSM (37.84%) and SSM-VGN (37.84%, p ≥ 0.05). Superficial spreading melanoma has in MTCs the highest incidence (46.43%, p ≥ 0.05). PD-L1 (+/-) expressions in TILs were observed in strong association with CD8+ and CD4+ T-lymphocytes (X2 = 11.238, p = 0.003; X2 = 12.614, p = 0.001, Table 2).
PD-L1 (+) patterns associated with moderate to severe CD8+ and CD4+ lymphocytes intensities (CD8+TILs: 69.57%; MTCs: 75.00%; CD4+TILs: 97.30%; MTCs: 85.71%) compared to mild (CD8+TILs: 2.79%; MTCs: 7.14%, CD4+TILs: 0.00%; MTCs: 3.57%, p < 0.05) were observed in the majority of morphological types of melanomas (Table 2) (Figure 1, Figure 2, Figure 3, Figure 4 and Figure 5). Melanomas negative for PD-L1 show a significant degree of staining (2+) by CD4 and CD8 infiltrating lymphocytes (CD8+TILs: 58.53%; MTCs: 75.76%, CD4+TILs: 70.83%; MTCs: 87.88%, p < 0.05).
Although PD-L1 expression associated with no TILs was not observed in melanoma tumor cells (X2 = 2.775, p = 0.514), PD-L1 was expressed in T lymphocytes (X2 = 8.383, p = 0.020). In our study, PD-L1 showed intertumoral heterogeneity. PD-L1, CD4 TILs, and CD8TILs biomarkers intensities were observed in Figure 1, Figure 2, Figure 3, Figure 4 and Figure 5.

3.3. Predictive and Prognostic Roles of PD-L1, CD4, and CD8 Tumor-Infiltrating Lymphocytes Biomarkers in Melanoma Tumor Microenvironment Cells

To establish the laboratory diagnostic, the predictive model (ROC curves) is used to estimate the risk of patient adverse outcomes in medical research and to use as a discriminatory tool for true positive values (sensitivity) and true negative values (specificity) [45]. ROC curves were used to show the accuracy of the methods by interpreting the true positive results (TPR, sensitivity) and false positive results (FPR, 100-specificity) for each biomarker with two overlapping distributions (negative, positive) (Table 3, Figure 6). Sensitivities of methods in patients with melanoma were very good for PD-L1, CD8TILs, and CD4TILs biomarkers (TILs: 97.30%; M: 96.43%, 89.13%, and 84.31%, p < 0.001 (Table 3, Figure 6). Also, specificities of analyzed biomarkers in melanoma tumor microenvironment cells were very good for PD-L1 (TILs: 91.67%; M: 96.97%), CD8 (86.67%), CD4 (90.00%, p < 0.001) (Table 3, Figure 6). The area under the curve (AUC) for our studied biomarkers (PD-L1, CD4 TILs, and CD8 TILs) starts from 0.941 to 0.975, which means a very good ability of the ROC test to discriminate the presence or absence of malign tumoral melanoma cells.
Furthermore, multivariate analysis Cox-proportional hazard regression analyzed the following parameters as the PD-L1, CD4, and CD8 TILs expression patterns to observe their potential prognostic roles in melanoma tumor microenvironment cells (Table 4). In our analysis, we observed that PDL-1 represents a favorable prognostic biomarker, an independent predictor factor implied in the prognosis of the melanoma patients.

4. Discussion

Immunotherapy has proved in the past few years to have a real success for many types of cancer. So far, the most effective immunotherapies are the monoclonal antibodies targeting the checkpoint molecules CTLA-4 (cytotoxic T lymphocytes-associated protein 4) and PD-1 (programmed cell death protein 1) and its ligand PD-L1. Antibody blockade of PD-1 and its ligand PD-L1 have antitumor efficacy and durability of responses in melanoma patients [14]. PD-L1 expression by melanoma tumor cells is heterogeneous and contiguous to tumor-infiltrating lymphocyte areas [11]. Melanomas may express PD-L1 against antitumor immune effector cells, facilitating immune evasion even if the B7-H1 costimulatory molecule is present on the surface of tumor cells. This regulates the cellular and humoral immune responses through the PD-1 receptor on activated T and B cells. Also, it was observed that in vitro, the B7-H1 tumor cell lines might increase the apoptosis of antigen-specific human T-cells, which means the apoptotic effects of B7-H1 are mediated by one or more receptors other than PD-1 [10]. TILs recognize tumors and secrete pro-inflammatory cytokines with a role in the upregulation of PD-L1 expression in the TME. PD-L1 ligates PD-1 on TILs determining their downregulation [47]. The PD-L1 expression in TILs is interesting to keep in observation because PD-L1 was correlated with different degrees of lymphocytic infiltration described by the Clark system (X2 = 8.383, p = 0.020), according to references [48].
PD-L1 (+) expression shows a higher incidence in cases with superficial spreading melanoma, superficial spreading melanoma with vertical growth nodules, and a lower incidence rate in lentigo maligna melanoma and nodular melanomas, in conformity with references [49,50].
In the studies on melanomas, a high Breslow tumor thickness represents an indicator of poor prognosis [51,52,53]. PD-L1 expression was observed often in melanoma cases with more than 2−4 mm of Breslow tumor thickness [54,55]. According to the references, our study shows a significant correlation between the PD-L1 expression and Breslow tumor thickness in melanoma tumor cells (X2 = 9.933, p = 0.014).
Tumor-infiltrating lymphocytes (TILs) infiltrate tumor cells. The degree of infiltration is described by the TIL infiltrate’s extent and intensity. The most applied grading scheme for assessing the presence of TILs is the Clark system, as follows: 1; absent TILs infiltrate. 2; non-brisk TILs infiltrate: focal areas of lymphocytic infiltration in the tumor. 3; brisk TILs infiltrate: TILs infiltration of the entire tumor base or diffuse permeation of the tumor. Other systems for grading TILs infiltrates have been proposed based on their density and distribution but have not been validated [51,52,53]. TILs are an essential histopathological feature in diagnosing melanocytic tumors, composed of a series of cells, including T lymphocytes [56].
CD8+ cytotoxic and CD4+ T cells are important for the action of immune checkpoint inhibitors [38]. PD-L1 (+/-) expression patterns were observed on both tumor and immune cells. They were differentiated in the function of morphologic features, and tumors were considered to be PD-L1 positive when ≥5% of tumor cells presented membrane staining [57,58]. The presence of CD8+ TILs was scored as 0 (negative), weak (1+, positive cells focally at the edge of tumor or perivascular, 5% of tumor area), moderate (2+, infiltrating tumor by extending away from intratumoral vessels, 5−50% of tumor area), or intense (3+, broad infiltration by TILs, more than 50% of tumor area). Our study presented that PD-L1 expression was associated with CD8 TILs and CD4 TILs in the tumor immune microenvironment (X2 = 11.238, p = 0.003; X2 = 12.614, p = 0.001). These results are in accordance with multiple previous studies showing how PD-L1 positive expression represents an adaptive mechanism of expression induced by the host immune antitumor response [40,50,59,60].
In melanoma, PD-L1 is expressed by activated lymphocytes, presumably to decrease inflammation [61,62]. PD-L1 in stromal cells interacts with PD-1 on effector T cells, inhibiting their function. Moreover, in the tumor microenvironment, PD-L1 expression might be induced by interferon-gamma secretion via effector T cells [12]. Responding to immune checkpoint inhibitors may depend on active interaction between PD-1 on CD8 cells and PD-L1 in stromal cells [63].
PD-L1 plays an important role in the immune response regulation in the tumor microenvironment [64]. When PD-L1 binds to PD-1, it determines the T cell proliferation inhibition and its secretion of cytokines [65]. PD-1/PD-L1 blockade represents an essential strategy with therapeutic goals [66].
PD-L1 status was examined in TILs and MTCs cells, and cut-off values were calculated by the ROC curve method. Sensitivities of methods for PD-L1, CD8, and CD4 biomarkers, in patients with melanoma, were from 84.31% to 97.30%, and specificities of these analyzed biomarkers in melanoma tumor microenvironment cells were from 86.67% to 96.97%, offering the possibility to be biomarkers with predictive accuracy in melanomas. The area under the curve (AUC) for PD-L1, CD4, and CD8 biomarkers was from 0.941 to 0.975, which means a very good ability of the ROC test to discriminate the presence or absence of malign tumoral melanoma cells. To obtain more accurate measurements of PD-L1 expression, an IHC quantitative method associated with TILs characterization by CD4 and CD8 TILs expression was used, in conformity with other studies [67,68,69]. To verify antibody specificity, we used quality negative and positive controls for PD-L1 containing placental and tonsil tissue as previously described studies [57,67]. Many clinical assays develop predictive biomarkers by comparing the expression of PD-L1 in the tumor microenvironment cells in samples from patients treated with immune checkpoint inhibitors. Expressing these biomarkers in the tumor microenvironment predicts a better response to therapy [39].
One of the challenges to assessing the potential role of PD-L1 expression as a predictive biomarker was the variation in assays used across studies. Most studies used chromogenic IHC assays and obtained results of PD-L1 expression were interpreted by pathologists by reporting the percentage of tumor cells and immune cells demonstrating expression [14]. Other predictive biomarker studies involving PD-L1 expression used standard immunohistochemistry (IHC) as references [63,70,71,72].
We assessed the PD-L1, CD4, and CD8 lymphocytes infiltrations expression patterns, lymphovascular invasion, and perineural invasion in MTCs by multivariate analysis of Cox-proportional hazard regression. We observed that PDL-1 represents a favorable prognostic biomarker, being an independent predictor factor implied in the prognosis of the melanoma patients.
The association between PD-L1 expression and prognosis in melanoma has been explored extensively in previous studies [32,33,40,57]. PD-L1 expression was an independent predictor of good prognosis in patients with melanomas. In some studies, PD-L1 expression was correlated with the treatment response, but other studies show it must be complemented by additional biomarkers for immune profiling of the CD8+ T cells [32,40,50,58,73].
Other studies demonstrated that a high expression rate of PD-L1 with a dense and diffuse T-cell inflammatory infiltrate is a characteristic feature of melanoma and represents a good prognostic marker for metastatic melanoma [74,75,76]. Adaptive PD-L1 expression in melanoma microenvironment pretreatment is associated with a good prognosis [57] and biomarker predictive of response to anti-PD-1/PD-L1 therapies in melanoma [38,70,72].
It is unclear if the PD-L1 negative status in advanced melanoma determinates phenotypic differences in immune responses or is an intrinsic tumor quality, and PD-L1–negative tumors are frequently associated with TILs. Melanoma with PD-L1–negative expression has a worse prognosis and responds less frequently to immune checkpoint therapies [11].
Lymphovascular invasion represents the presence of melanoma cells within the lamina of blood vessels or lymphatics, or both and is recorded as absent or present. Neurotropism represents the presence of melanoma cells close to the nerve sheaths. It is best identified at the periphery of the tumor and is associated with an increased local recurrence rate or local persistence. The density of lymphatic vessels within or surrounding growing tumors correlates with poor patient outcomes, being observed in many types of solid tumors [77,78]. Our data shows that lymphovascular and perineural invasion, both reliable morphologic prognostic features, did not correlate with PD-L1 expression and may not be considered an unfavorable prognostic marker.
Many studies have investigated the impact of PD-L1 on the prognosis of solid tumors [79]. PD-L1 expression was an independent prognostic factor in renal cell carcinoma [80]. A high PD-L1 expression was a poor prognostic biomarker in patients with non-Hodgkin lymphoma [5,81].
Our results might have limitations such as a limited sampling, the lack of consensus method in the evaluation of PD-L1 expression (only quantitative in our study), not considering more categories of positivity depending on the amount and distribution of positive melanoma cells, and the fact that the immunohistochemical tests were performed on tissue fragments prior to any treatment. Other study limitations in the association between PD-L1 and melanoma prognosis are the heterogeneity, patient ethnicity, and treatment, which could influence patient survival. In further studies, we propose working with a larger cohort of melanoma patients to have meaningful results to validate the immuno-histochemical PD-L1 expression with roles in diagnosis and prognosis.

5. Conclusions

In this study, we used a melanoma patient cohort to characterize the PD-L1 expression in melanoma in the context of T-cell infiltrates because the PD-1/PD-L1 blockade represents the target in treating melanoma strategy. Most of the PD-L1 positive expressing tumors have a moderate score of CD4+ TILs and CD8+TILs (5-50% of tumor area) in tumoral melanoma environment cells. PD-L1 expression represents a predictive biomarker with very good accuracy to discriminate the presence or absence of malign tumoral melanoma cells. PD-L1 expression was an independent predictor of good prognosis in patients with melanomas.

Author Contributions

All authors contributed equally to this study and shared the first authorship. Conceptualization, B.M.C., A.-A.N., E.M., M.E., M.A., M.D., E.G., L.-T.H. and G.C.C.; methodology, M.E., M.A., G.I.B., A.-A.N., A.C. and M.D.; software, E.M., L.-T.H., N.D. and L.P.; validation, E.M., L.-T.H.; formal analysis, M.E.; investigation B.M.C., A.-A.N., G.I.B., M.E. and C.I.O.; resources, A.-A.N. and M.E.; writing—original draft preparation, E.M., A.-A.N., B.M.C., M.E., A.C., M.A., M.D., M.C.R. and G.C.C.; writing—review and editing, E.M., A.-A.N. and M.E.; visualization, N.D. and G.C.C.; supervision, M.D. and M.A.; project administration, A.-A.N. and M.E.; funding acquisition, A.-A.N., M.D. and G.C.C. All authors have read and agreed to the published version of the manuscript.

Funding

This study is part of the result indicators assumed within a SEPMEL grant, contract number 06/14 November 2018. This grant was won in the biomedical competition organized into the CNFIS-FDI-2018-0462 project entitled “Excellence, performance, and competitiveness in biomedical research at the Ovidius University of Constanta”.

Institutional Review Board Statement

All aspects of ethics approval and consent to participate of patients in this study are made in conformity with the declaration of Helsinki 2000 and approved by the Ethics Committee of “Ovidius” University of Constanta, Romania, 5418/14 December 2018.

Informed Consent Statement

Informed consent was obtained from all patients involved in the study.

Data Availability Statement

Data are contained within the article.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. O’Neill, C.H.; Scoggins, C.R. Melanoma. J. Surg. Oncol. 2019, 120, 873–881. [Google Scholar] [CrossRef] [PubMed]
  2. Rodić, N.; Anders, R.A.; Eshleman, J.R.; Lin, M.-T.; Xu, H.; Kim, J.H.; Beierl, K.; Chen, S.; Luber, B.S.; Wang, H.; et al. PD-L1 expression in melanocytic lesions does not correlate with the BRAF V600E mutation. Cancer Immunol. Res. 2015, 3, 110–115. [Google Scholar] [CrossRef]
  3. Fujimura, T.; Fujisawa, Y.; Kambayashi, Y.; Aiba, S. Significance of BRAF kinase inhibitors for melanoma treatment: From bench to bedside. Cancers 2019, 11, 1342. [Google Scholar] [CrossRef] [PubMed]
  4. Weiss, S.A.; Wolchok, J.D.; Sznol, M. Immunotherapy of melanoma: Facts and hopes. Clin Cancer Res. 2019, 25, 5191–5201. [Google Scholar] [CrossRef]
  5. Yang, J.; Dong, M.; Shui, Y.; Zhang, Y.; Zhang, Z.; Mi, Y.; Zuo, X.; Jiang, L.; Liu, K.; Liu, Z.; et al. A pooled analysis of the prognostic value of PD-L1 in melanoma: Evidence from 1062 patients. Cancer Cell Int. 2020, 20, 96. [Google Scholar] [CrossRef]
  6. Chen, J.; Jiang, C.C.; Jin, L.; Zhang, X.D. Regulation of PD-L1: A novel role of pro-survival signalling in cancer. Ann. Oncol. 2016, 27, 409–416. [Google Scholar] [CrossRef] [PubMed]
  7. Wei, R.; Guo, L.B.; Wang, Q.S.; Miao, J.; Kwok, H.F.; Lin, Y. Targeting PD-L1 protein: Translation, modification and transport. Curr. Protein Pept. Sci. 2019, 20, 82–91. [Google Scholar] [CrossRef]
  8. Jiang, X.J.; Wang, J.; Deng, X.Y.; Xiong, F.; Ge, J.S.; Xiang, B.; Wu, X.; Ma, J.; Zhou, M.; Li, X.L.; et al. Role of the tumor microenvironment in PD-L1/PD-1-mediated tumor immune escape. Mol. Cancer 2019, 18, 1–7. [Google Scholar] [CrossRef]
  9. Chen, L.P.; Han, X. Anti-PD-1/PD-L1 therapy of human cancer: Past, present, and future. J. Clin. Investig. 2015, 125, 3384–3391. [Google Scholar] [CrossRef] [PubMed]
  10. Dong, H.; Strome, S.E.; Salomao, D.R.; Tamura, H.; Hirano, F.; Flies, D.B.; Roche, P.C.; Lu, J.; Zhu, G.; Tamada, K.; et al. Tumor-associated B7-H1 promotes T-cell apoptosis: A potential mechanism of immune evasion. Nat. Med. 2002, 8, 793–800. [Google Scholar] [CrossRef]
  11. Madore, J.; Strbenac, D.; Vilain, R.; Menzies, A.M.; Yang, J.Y.H.; Thompson, J.F.; Long, G.V.; Mann, G.J.; Scolyer, R.A.; Wilmott, J.S. PD-L1 Negative Status is Associated with Lower Mutation Burden, Differential Expression of Immune-Related Genes, and Worse Survival in Stage III Melanoma. Clin. Cancer Res. 2016, 22, 3915–3923. [Google Scholar] [CrossRef]
  12. Robert, C.; Long, G.V.; Brady, B.; Dutriaux, C.; Maio, M.; Mortier, L.; Hassel, J.C.; Rutkowski, P.; McNeil, C.; Kalinka-Warzocha, E.; et al. Nivolumab in previously untreated melanoma without BRAF mutation. N. Engl. J. Med. 2015, 372, 320–330. [Google Scholar] [CrossRef] [PubMed]
  13. Weber, J.S.; D’Angelo, S.P.; Minor, D.; Hodi, F.S.; Gutzmer, R.; Neyns, B.; Hoeller, C.; Khushalani, N.I.; Miller, W.H., Jr.; Lao, C.D.; et al. Nivolumab versus chemotherapy in patients with advanced melanoma who progressed after anti-CTLA-4 treatment (CheckMate 037): A randomized, controlled, open-label, phase 3 trial. Lancet Oncol. 2015, 16, 375–384. [Google Scholar] [CrossRef] [PubMed]
  14. Sunshine, J.C.; Nguyen, P.L.; Kaunitz, G.J.; Cottrell, T.R.; Berry, S.; Esandrio, J.; Xu, H.; Ogurtsova, A.; Bleich, K.B.; Cornish, T.C.; et al. PD-L1 Expression in Melanoma: A Quantitative Immunohistochemical Antibody Comparison. Clin. Cancer Res. 2017, 23, 4938–4944. [Google Scholar] [CrossRef]
  15. Yu, H.; Boyle, T.A.; Zhou, C.; Rimm, D.L.; Hirsch, F.R. PD-L1 expression in lung cancer. J. Thorac. Oncol. 2016, 11, 964–975. [Google Scholar] [CrossRef]
  16. Liu, Y.X.; Wang, X.S.; Wang, Y.F.; Hu, X.C.; Yan, J.Q.; Zhang, Y.L.; Wang, W.; Yang, R.J.; Feng, Y.Y.; Gao, S.G.; et al. Prognostic significance of PD-L1 expression in patients with gastric cancer in East Asia: A meta-analysis. Oncotarg. Ther. 2016, 9, 2649–2654. [Google Scholar]
  17. Huang, L.J.; Deng, X.F.; Chang, F.; Wu, X.L.; Wu, Y.; Diao, Q.Z. Prognostic significance of programmed cell death ligand 1 expression in patients with ovarian carcinoma A systematic review and meta-analysis. Medicine 2018, 97, 43. [Google Scholar]
  18. Wu, Z.; Zhang, L.; Peng, J.; Xu, S.; Zhou, L.; Lin, Y.; Wang, Y.; Lu, J.; Yin, W.; Lu, J. Predictive and prognostic value of PDL1 protein expression in breast cancer patients in neoadjuvant setting. Cancer Biol. Ther. 2019, 20, 941–947. [Google Scholar] [CrossRef]
  19. Gevensleben, H.; Dietrich, D.; Golletz, C.; Steiner, S.; Jung, M.; Thiesler, T.; Majores, M.; Stein, J.; Uhl, B.; Muller, S.; et al. The immune checkpoint regulator PD-L1 is highly expressed in aggressive primary prostate cancer. Clin. Cancer Res. 2016, 22, 1969–1977. [Google Scholar] [CrossRef] [PubMed]
  20. Wang, B.; Pan, W.; Yang, M.; Yang, W.; He, W.; Chen, X.; Bi, J.; Jiang, N.; Huang, J.; Lin, T. Programmed death ligand-1 is associated with tumor infiltrating lymphocytes and poorer survival in urothelial cell carcinoma of the bladder. Cancer Sci. 2019, 110, 489–498. [Google Scholar] [CrossRef]
  21. Gu, X.; Dong, M.; Liu, Z.; Mi, Y.; Yang, J.; Zhang, Z.; Liu, K.; Jiang, L.; Zhang, Y.; Dong, S.; et al. Elevated PD-L1 expression predicts poor survival outcomes in patients with cervical cancer. Cancer Cell Int. 2019, 19, 146. [Google Scholar] [CrossRef]
  22. Lee, L.H.; Cavalcanti, M.S.; Segal, N.H.; Hechtman, J.F.; Weiser, M.R.; Smith, J.J.; Garcia-Aguilar, J.; Sadot, E.; Ntiamoah, P.; Markowitz, A.J.; et al. Patterns and prognostic relevance of PD-1 and PD-L1 expression in colorectal carcinoma. Mod. Pathol. 2016, 29, 1433–1442. [Google Scholar] [CrossRef]
  23. Liang, X.; Sun, J.; Wu, H.; Luo, Y.; Wang, L.; Lu, J.; Zhang, Z.; Guo, J.; Liang, Z.; Liu, T. PD-L1 in pancreatic ductal adenocarcinoma: A retrospective analysis of 373 Chinese patients using an in vitro diagnostic assay. Diagn. Pathol. 2018, 13, 5. [Google Scholar] [CrossRef] [PubMed]
  24. Motoshima, T.; Komohara, Y.; Ma, C.; Dewi, A.K.; Noguchi, H.; Yamada, S.; Nakayama, T.; Kitada, S.; Kawano, Y.; Takahashi, W.; et al. PD-L1 expression in papillary renal cell carcinoma. BMC Urol. 2017, 17, 8. [Google Scholar] [CrossRef] [PubMed]
  25. Cho, J.; Ahn, S.; Yoo, K.H.; Kim, J.H.; Choi, S.H.; Jang, K.T.; Lee, J. Treatment outcome of PD-1 immune checkpoint inhibitor in Asian metastatic melanoma patients: Correlative analysis with PD-L1 immunohistochemistry. Invest. N. Drugs 2016, 34, 677–684. [Google Scholar] [CrossRef] [PubMed]
  26. Gadiot, J.; Hooijkaas, A.I.; Kaiser, A.D.M.; van Tinteren, H.; van Boven, H.; Blank, C. Overall survival and PD-L1 expression in metastasized malignant melanoma. Cancer 2011, 117, 2192–2201. [Google Scholar] [CrossRef] [PubMed]
  27. Johnson, D.B.; Bordeaux, J.; Kim, J.Y.; Vaupel, C.; Rimm, D.L.; Ho, T.H.; Joseph, R.W.; Daud, A.I.; Conry, R.M.; Gaughan, E.M.; et al. Quantitative spatial profiling of PD-1/PD-L1 interaction and HLA-DR/IDO-1 predicts improved outcomes of anti-PD-1 therapies in metastatic melanoma. Clin. Cancer Res. 2018, 24, 5250–5260. [Google Scholar] [CrossRef] [PubMed]
  28. Madonna, G.; Ballesteros-Merino, C.; Feng, Z.; Bifulco, C.; Capone, M.; Giannarelli, D.; Mallardo, D.; Simeone, E.; Grimaldi, A.M.; Caraco, C.; et al. PD-L1 expression with immune-infiltrate evaluation and outcome prediction in melanoma patients treated with ipilimumab. Oncoimmunology 2018, 7, e1405206. [Google Scholar] [CrossRef]
  29. Madore, J.; Vilain, R.E.; Menzies, A.M.; Kakavand, H.; Wilmott, J.S.; Hyman, J.; Yearley, J.H.; Kefford, R.F.; Thompson, J.F.; Long, G.V.; et al. PD-L1 expression in melanoma shows marked heterogeneity within and between patients: Implications for anti-PD-1/PD-L1 clinical trials. Pigment Cell Melanoma Res. 2015, 28, 245–253. [Google Scholar] [CrossRef] [PubMed]
  30. Massi, D.; Brusa, D.; Merelli, B.; Falcone, C.; Xue, G.; Carobbio, A.; Nassini, R.; Baroni, G.; Tamborini, E.; Cattaneo, L.; et al. The status of PD-L1 and tumor infiltrating immune cells predict resistance and poor prognosis in BRAF treated melanoma patients harboring mutant BRAFV600. Ann. Oncol. 2015, 26, 1980–1987. [Google Scholar] [CrossRef] [PubMed]
  31. Morrison, C.; Pabla, S.; Conroy, J.M.; Nesline, M.K.; Glenn, S.T.; Dressman, D.; Papanicolau-Sengos, A.; Burgher, B.; Andreas, J.; Giamo, V.; et al. Predicting response to checkpoint inhibitors in melanoma beyond PD-L1 and mutational burden. J. Immunother. Cancer 2018, 6, 32. [Google Scholar] [CrossRef] [PubMed]
  32. Obeid, J.M.; Erdag, G.; Smolkin, M.E.; Deacon, D.H.; Patterson, J.W.; Chen, L.; Bullock, T.N.; Slingluff, C.L. PD-L1, PD-L2 and PD-1 expression in metastatic melanoma: Correlation with tumor-infiltrating immune cells and clinical outcome. Oncoimmunology 2016, 5, e1235107. [Google Scholar] [CrossRef] [PubMed]
  33. Ren, M.; Dai, B.; Kong, Y.Y.; Lv, J.J.; Cai, X. PD-L1 expression in tumour-infiltrating lymphocytes is a poor prognostic factor for primary acral melanoma patients. Histopathology 2018, 73, 386–396. [Google Scholar] [CrossRef]
  34. Ren, Y.; Lv, Q.; Yue, W.; Liu, B.; Zou, Z. The programmed cell death protein-1/programmed cell death ligand 1 expression, CD3+ T cell infiltration, NY-ESO-1 expression, and microsatellite instability phenotype in primary cutaneous melanoma and mucosal melanoma and their clinical significance and prognostic value: A study of 89 consecutive cases. Melanoma Res. 2019, 30, 85–101. [Google Scholar]
  35. Schaper-Gerhardt, K.; Okoye, S.; Herbst, R.; Ulrich, J.; Terheyden, P.; Pfohler, C.; Utikal, J.S.; Kreuter, A.; Mohr, P.; Dippel, E.; et al. PD-L1 status does not predict the outcome of BRAF inhibitor therapy in metastatic melanoma. Eur. J. Cancer 2018, 88, 67–76. [Google Scholar] [CrossRef]
  36. Thierauf, J.; Veit, J.A.; Affolter, A.; Bergmann, C.; Grunow, J.; Laban, S.; Lennerz, J.K.; Grunmuller, L.; Mauch, C.; Plinkert, P.K.; et al. Identification and clinical relevance of PD-L1 expression in primary mucosal malignant melanoma of the head and neck. Melanoma Res. 2015, 25, 503–509. [Google Scholar] [CrossRef] [PubMed]
  37. Wang, H.Y.; Wu, X.Y.; Zhang, X.; Yang, X.H.; Long, Y.K.; Feng, Y.F.; Wang, F. Prevalence of NRAS mutation, PD-L1 expression and amplification, and overall survival analysis in 36 primary vaginal melanomas. Oncologist 2019, 25, e291–e301. [Google Scholar] [CrossRef]
  38. Tumeh, P.C.; Harview, C.L.; Yearley, J.H.; Shintaku, I.P.; Taylor, E.J.M.; Robert, L.; Chmielowski, B.; Spasic, M.; Henry, G.; Ciobanu, V.; et al. PD-1 blockade induces responses by inhibiting adaptive immune resistance. Nature 2014, 515, 568–571. [Google Scholar] [CrossRef] [PubMed]
  39. Kluger, H.M.; Zito, C.R.; Turcu, G.; Baine, M.K.; Zhang, H.; Adeniran, A.; Sznol, M.; Rimm, D.L.; Kluger, Y.; Chen, L.; et al. PD-L1 Studies Across Tumor Types, its Differential Expression and Predictive Value in Patients Treated with Immune Checkpoint Inhibitors. Clin. Cancer Res. 2017, 23, 4270–4279. [Google Scholar] [CrossRef] [PubMed]
  40. Kakavand, H.; Rawson, R.V.; Pupo, G.M.; Yang, J.Y.H.; Menzies, A.M.; Carlino, M.S.; Kefford, R.F.; Howle, J.R.; Saw, R.P.; Thompson, J.F.; et al. PD-L1 expression and immune escape in melanoma resistance to MAPK inhibitors. Clin. Cancer Res. 2017, 23, 6054–6061. [Google Scholar] [CrossRef] [PubMed]
  41. Cancer Genome Atlas Network. Genomic Classification of Cutaneous Melanoma. Cell 2015, 161, 1681–1696. [Google Scholar] [CrossRef] [PubMed]
  42. Ionescu, C.A.; Aschie, M.; Matei, E.; Cozaru, G.C.; Deacu, M.; Mitroi, A.F.; Baltatescu, G.I.; Nicolau, A.A.; Mazilu, L.; Tuta, L.A.; et al. Characterization of the Tumor Microenvironment and the Biological Processes with a Role in Prostatic Tumorigenesis. Biomedicines 2022, 10, 1672. [Google Scholar] [CrossRef] [PubMed]
  43. Bosoteanu, C.; Bosoteanu, M.; Aschie, M. Differential diagnosis issues in a case of gastric carcinoma associated with leukemoid reaction. Rom. J. Morphol. Embryol. 2009, 50, 701–705. [Google Scholar]
  44. Goggins, W.B.; Finkelstein, D.M.; Tsao, H. Evidence for an association between cutaneous melanoma and non-Hodgkin lymphoma. Cancer 2001, 91, 874–880. [Google Scholar] [CrossRef] [PubMed]
  45. Matei, E.; Aschie, M.; Mitroi, A.F.; Ghinea, M.M.; Gheorghe, E.; Petcu, L.; Dobrin, N.; Chisoi, A.; Mihaela, M. Biomarkers involved in evaluation of platelets function in South-Eastern Romanian patients with hematological malignancies subtypes. Medicine 2021, 100, e25944. [Google Scholar] [CrossRef] [PubMed]
  46. Brinzan, C.; Așchie, M.; Matei, E.; Mitroi, A.F.; Cozaru, G.C. Molecular expression profiles of selected microRNAs in colorectal adenocarcinoma in patients from south-eastern part of Romania. Medicine 2019, 98, e18122. [Google Scholar] [CrossRef]
  47. Karydis, I.; Chan, P.Y.; Wheater, M.; Arriola, E.; Szlosarek, P.W.; Ottensmeier, C. Clinical activity and safety of Pembrolizumab in Ipilimumab pre-treated patients with uveal melanoma. Oncoimmunology 2016, 5, e1143997. [Google Scholar] [CrossRef]
  48. Lee, S.-J.; Lim, H.J.; Choi, Y.H.; Chang, Y.H.; Lee, W.J.; Kim, D.W.; Yoon, G.S. The clinical significance of tumor infiltrating lymphocytes and microscopic satellites in acral melanoma in a Korean population. Ann. Dermatol. 2013, 25, 61–66. [Google Scholar] [CrossRef]
  49. Song, H.; Wu, Y.; Ren, G.; Guo, W.; Wang, L. Prognostic factors of oral mucosal melanoma: Histopathological analysis in a retrospective cohort of 82 cases. Histopathology 2015, 67, 548–556. [Google Scholar] [CrossRef]
  50. Kaunitz, G.J.; Cottrell, T.R.; Lilo, M.; Muthappan, V.; Esandrio, J.; Berry, S.; Xu, H.; Ogurtsova, A.; Anders, R.A.; Fischer, A.H.; et al. Melanoma subtypes demonstrate distinct PD-L1 expression profiles. Lab. Investig. 2017, 97, 1063–1071. [Google Scholar] [CrossRef]
  51. Elder, D.E.; Massi, D.; Scolyer, R.; Willemze, R. WHO Classification of Skin Tumours, 4th ed.; World Health Organization: Geneva, Switzerland, 2018. [Google Scholar]
  52. Elder, D.E. Lever’s Histopathology of the Skin, 11th ed.; Wolters-Kluwer: Alphen aan den Rijn, The Netherlands, 2014; pp. 488–547. [Google Scholar]
  53. Calonje, E.; Brenn, T.; Lazar, A. McKee PH McKee’s Pathology of the Skin, 4th ed.; Elsevier: Amsterdam, The Netherlands, 2012; pp. 1126–1267. [Google Scholar]
  54. Massi, D.; Brusa, D.; Merelli, B.; Ciano, M.; Audrito, V.; Serra, S.; Buonincontri, R.; Baroni, G.; Nassini, R.; Minocci, D.; et al. PD-L1 marks a subset of melanomas with a shorter overall survival and distinct genetic and morphological characteristics. Ann. Oncol. 2014, 25, 2433–2442. [Google Scholar] [CrossRef]
  55. Schramm, S.J.; Mann, G.J. Melanoma prognosis: A REMARK-based systematic review and bioinformatic analysis of immunohistochemical and gene microarray studies. Mol. Cancer Ther. 2011, 10, 1520–1528. [Google Scholar] [CrossRef]
  56. Nicolau, A.A.; Caraban, B.M.; Gheorghe, E.; Hangan, T.L.; Cojocaru, O.; Orasanu, C.I.; Voda, R.I. Proteomic Implications of Tumoral Infiltrating Lymphocytes in Melanoma: PD-L1, CD4 and CD8—Short Review. ARS Med. Tomitana 2020, 3, 117–121. [Google Scholar] [CrossRef]
  57. Taube, J.M.; Anders, R.A.; Young, G.D.; Xu, H.; Sharma, R.; McMiller, T.L.; Chen, S.; Klein, A.P.; Pardoll, D.M.; Topalian, S.L.; et al. Colocalization of inflammatory response with B7-H1 expression in human melanocytic lesions supports an adaptive resistance mechanism of immune escape. Sci. Transl. Med. 2012, 4, 127ra37. [Google Scholar] [CrossRef] [PubMed]
  58. Taube, J.M.; Klein, A.; Brahmer, J.R.; Xu, H.; Pan, X.; Kim, J.H.; Chen, L.; Pardoll, D.M.; Topalian, S.L.; Anders, R.A. Association of PD-1, PD-1 ligands, and other features of the tumor immune microenvironment with response to anti-PD-1 therapy. Clin. Cancer Res. 2014, 20, 5064–5074. [Google Scholar] [CrossRef]
  59. Ahmadzadeh, M.; Johnson, L.A.; Heemskerk, B.; Wunderlich, J.R.; Dudley, M.E.; White, D.E.; Rosenberg, S.A. Tumor antigen-specific CD8 T cells infiltrating the tumor express high levels of PD-1 and are functionally impaired. Blood 2009, 114, 1537–1544. [Google Scholar] [CrossRef] [PubMed]
  60. Mandala, M.; Merelli, B.; Massi, D. PD-L1 in melanoma: Facts and myths. Melanoma Manag. 2016, 3, 187–194. [Google Scholar] [CrossRef]
  61. Bønnelykke-Behrndtz, M.L.; Steiniche, T.; Damsgaard, T.E.; Georgsen, J.B.; Danielsen, A.; Bastholt, L.; Møller, H.J.; Nørgaard, P.H.; Schmidt, H. Melanoma negative spindle-cell associated melanoma, a distinct inflammatory phenotype correlated with dense infiltration of CD163 macrophages and loss of E-cadherin. Melanoma Res. 2015, 25, 113–118. [Google Scholar] [CrossRef]
  62. Le, D.T.; Uram, J.N.; Wang, H.; Bartlett, B.R.; Kemberling, H.; Eyring, A.D.; Skora, A.D.; Luber, B.S.; Azad, N.S.; Laheru, D.; et al. PD-1 blockade in tumors with mismatch-repair deficiency. N. Engl. J. Med. 2015, 372, 2509–2520. [Google Scholar] [CrossRef]
  63. Robert, C.; Ribas, A.; Wolchok, J.D.; Hodi, F.S.; Hamid, O.; Kefford, R.; Weber, J.S.; Joshua, A.M.; Hwu, W.-J.; Gangadhar, T.C.; et al. Anti-programmed-death-receptor 1 treatment with pembrolizumab in ipilimumab-refractory advanced melanoma: A randomised dose-comparison cohort of a phase 1 trial. Lancet 2014, 384, 1109–1117. [Google Scholar] [CrossRef] [PubMed]
  64. Dermani, F.K.; Samadi, P.; Rahmani, G.; Kohlan, A.K.; Najafi, R. PD-1/PD-L1 immune checkpoint: Potential target for cancer therapy. J. Cell Physiol. 2019, 234, 1313–1325. [Google Scholar] [CrossRef]
  65. Zou, W.P.; Wolchok, J.D.; Chen, L.P. PD-L1 (B7-H1) and PD-1 pathway blockade for cancer therapy: Mechanisms, response biomarkers, and combinations. Sci. Transl. Med. 2016, 8, 328. [Google Scholar] [CrossRef]
  66. Clark, C.A.; Gupta, H.B.; Sareddy, G.; Pandeswara, S.; Lao, S.; Yuan, B.; Drerup, J.M.; Padron, A.; Conejo-Garcia, J.; Murthy, K.; et al. Tumor-intrinsic PD-L1 signals regulate cell growth, pathogenesis, and autophagy in ovarian cancer and melanoma. Cancer Res. 2016, 76, 6964–6974. [Google Scholar] [CrossRef]
  67. Jilaveanu, L.B.; Shuch, B.; Zito, C.R.; Parisi, F.; Barr, M.; Kluger, Y.; Chen, L.; Kluger, H.M. PD-L1 expression in clear cell renal cell carcinoma: An analysis of nephrectomy and sites of metastases. J Cancer 2014, 5, 166–172. [Google Scholar] [CrossRef]
  68. Schalper, K.A.; Velcheti, V.; Carvajal, D.; Wimberly, H.; Brown, J.; Pusztai, L.; Rimm, D.L. In situ tumor PD-L1 mRNA expression is associated with increased TILs and better outcome in breast carcinomas. Clin. Cancer Res. 2014, 20, 2773–2782. [Google Scholar] [CrossRef] [PubMed]
  69. Velcheti, V.; Schalper, K.A.; Carvajal, D.E.; Anagnostou, V.K.; Syrigos, K.N.; Sznol, M.; Herbst, R.S.; Gettinger, S.N.; Chen, L.; Rimm, D.L. Programmed death ligand-1 expression in non-small cell lung cancer. Lab. Investig. 2014, 94, 107–116. [Google Scholar] [CrossRef]
  70. Topalian, S.L.; Sznol, M.; McDermott, D.F.; Kluger, H.M.; Carvajal, R.D.; Sharfman, W.H.; Brahmer, J.R.; Lawrence, D.P.; Atkins, M.B.; Powderly, J.D.; et al. Survival, durable tumor remission, and long-term safety in patients with advanced melanoma receiving nivolumab. J. Clin. Oncol. 2014, 32, 1020–1030. [Google Scholar] [CrossRef] [PubMed]
  71. Ribas, A.; Puzanov, I.; Dummer, R.; Schadendorf, D.; Hamid, O.; Robert, C.; Hodi, F.S.; Schachter, J.; Pavlick, A.C.; Lewis, K.D.; et al. Pembrolizumab versus investigator-choice chemotherapy for ipilimumab-refractory melanoma (KEYNOTE-002): A randomised, controlled, phase 2 trial. Lancet Oncol. 2015, 16, 908–918. [Google Scholar] [CrossRef] [PubMed]
  72. Topalian, S.L.; Drake, C.G.; Pardoll, D.M. Immune checkpoint blockade: A common denominator approach to cancer therapy. Cancer Cell 2015, 27, 450–461. [Google Scholar] [CrossRef]
  73. Kwong, F.N.K.; Laggner, U.; McKinney, O.; Croud, J.; Rice, A.; Nicholson, A.G. Expression of PD-L1 correlates with pleomorphic morphology and histological patterns of non-small-cell lung carcinomas. Histopathology 2018, 72, 1024–1032. [Google Scholar] [CrossRef]
  74. Ghebeh, H.; Mohammed, S.; Al-Omair, A.; Qattant, A.; Lehe, C.; Al-Qudaihi, G.; Elkum, N.; Alshabanah, M.; Bin Amer, S.; Tulbah, A.; et al. The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: Correlation with important high-risk prognostic factors. Neoplasia 2006, 8, 190–198. [Google Scholar] [CrossRef] [PubMed]
  75. Hino, R.; Kabashima, K.; Kato, Y.; Yagi, H.; Nakamura, M.; Honjo, T.; Okazaki, T.; Tokura, Y. Tumor cell expression of programmed cell death-1 ligand 1 is a prognostic factor for malignant melanoma. Cancer 2010, 116, 1757–1766. [Google Scholar] [CrossRef] [PubMed]
  76. Krönig, H.; Falchner, K.J.; Odendahl, M.; Brackertz, B.; Conrad, H.; Muck, D.; Hein, R.; Blank, C.; Peschel, C.; Haller, B.; et al. PD-1 expression on Melan-A-reactive T cells increases during progression to metastatic disease. Int. J. Cancer 2012, 130, 2327–2336. [Google Scholar] [CrossRef] [PubMed]
  77. Fujimoto, N.; Dieterich, L.C. Mechanisms and Clinical Significance of Tumor Lymphatic Invasion. Cells 2021, 10, 2585. [Google Scholar] [CrossRef] [PubMed]
  78. Brinzan, C.; Așchie, M.; Grasa, C.N.; Mitroi, A.F.; Matei, E.; Cozaru, G.C. The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort. Rev. Chim. 2019, 70, 1346–1350. [Google Scholar] [CrossRef]
  79. Iacovelli, R.; Nole, F.; Verri, E.; Renne, G.; Paglino, C.; Santoni, M.; Rocca, M.C.; Giglione, P.; Aurilio, G.; Cullura, D.; et al. Prognostic role of PD-L1 expression in renal cell carcinoma. a systematic review and meta-analysis. Target. Oncol. 2016, 11, 143–148. [Google Scholar] [CrossRef]
  80. Pyo, J.S.; Kang, G.; Kim, J.Y. Prognostic role of PD-L1 in malignant solid tumors: A meta-analysis. Int. J. Biol. Markers 2017, 32, E68–E74. [Google Scholar] [CrossRef]
  81. Zhao, S.; Zhang, M.H.; Zhang, Y.; Meng, H.X.; Wang, Y.; Liu, Y.P.; Jing, J.; Huang, L.; Sun, M.Q.; Zhang, Y.; et al. The prognostic value of programmed cell death ligand 1 expression in non-Hodgkin lymphoma: A meta-analysis. Cancer Biol. Med. 2018, 15, 290–298. [Google Scholar]
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Figure 1. In nodular polypoid melanoma, CD4 intense membranous expression in intra and peritumoral lymphocytes (CD4 monoclonal antibody, clone EP204). In the lower left side detail, obx200.
Figure 1. In nodular polypoid melanoma, CD4 intense membranous expression in intra and peritumoral lymphocytes (CD4 monoclonal antibody, clone EP204). In the lower left side detail, obx200.
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Figure 2. CD8 intense positive reaction in superficial spreading melanoma (CD8 Monoclonal Antibody, clone SP16, scanned image). In the lower right side, detail ob.x200.
Figure 2. CD8 intense positive reaction in superficial spreading melanoma (CD8 Monoclonal Antibody, clone SP16, scanned image). In the lower right side, detail ob.x200.
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Figure 3. PD-L1 negative expression in tumor cells and TILs of nodular melanoma (PD-L1 Monoclonal Antibody, Clone CAL10). The brown staining is due to melanin pigment and melanophages. In the lower right side, detail ob.x100.
Figure 3. PD-L1 negative expression in tumor cells and TILs of nodular melanoma (PD-L1 Monoclonal Antibody, Clone CAL10). The brown staining is due to melanin pigment and melanophages. In the lower right side, detail ob.x100.
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Figure 4. PD-L1 positive expression in melanoma cells of a superficial spreading melanoma (PD-L1 Monoclonal Antibody, Clone CAL10). Lower right side, detail, ob.x200.
Figure 4. PD-L1 positive expression in melanoma cells of a superficial spreading melanoma (PD-L1 Monoclonal Antibody, Clone CAL10). Lower right side, detail, ob.x200.
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Figure 5. PD-L1 negative expression in tumor cells and focally positive in TILs in a case of superficial spreading melanoma (PD-L1 Monoclonal Antibody, Clone CAL10). Lower right side, detail, ob.x400.
Figure 5. PD-L1 negative expression in tumor cells and focally positive in TILs in a case of superficial spreading melanoma (PD-L1 Monoclonal Antibody, Clone CAL10). Lower right side, detail, ob.x400.
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Figure 6. PD-L1, CD4, and CD8 tumor-infiltrating lymphocytes expressions patterns as potential biomarkers in melanoma diagnosis. Receiver operating curve (ROC) analyses were generated from 61 patients and had a value of the area under the curve (AUC) for PD-L1 (TILs: sensitivity: 97.30%, specificity: 91.67%; p < 0.001; M: sensitivity: 96.43%, specificity: 96.97%; p < 0.001), CD8 tumor-infiltrating lymphocytes (M: sensitivity: 89.13%, specificity: 86.67%; p < 0.001), and CD4 tumor-infiltrating lymphocytes (M: sensitivity: 84.31%, specificity: 90.00%; p < 0.001), respectively.
Figure 6. PD-L1, CD4, and CD8 tumor-infiltrating lymphocytes expressions patterns as potential biomarkers in melanoma diagnosis. Receiver operating curve (ROC) analyses were generated from 61 patients and had a value of the area under the curve (AUC) for PD-L1 (TILs: sensitivity: 97.30%, specificity: 91.67%; p < 0.001; M: sensitivity: 96.43%, specificity: 96.97%; p < 0.001), CD8 tumor-infiltrating lymphocytes (M: sensitivity: 89.13%, specificity: 86.67%; p < 0.001), and CD4 tumor-infiltrating lymphocytes (M: sensitivity: 84.31%, specificity: 90.00%; p < 0.001), respectively.
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Table 1. Melanoma tumor microenvironment characterization in the function of PD-L1 (+/-) expression.
Table 1. Melanoma tumor microenvironment characterization in the function of PD-L1 (+/-) expression.
Nb.Clinico-
Pathological Aspects		PD-L1 Expression in TILsX2
Score		p-ValuePD-L1 Expression in MTCsX2
Score		p-Value
Negative
n (%)		Positive
n (%)		Negative
n (%)		Positive
n (%)
1.Age
<65 years
>65 years
8 (33.33%)
16 (66.67%)
20 (54.05%)
17 (54.95%)
2.517
0.126
17 (51.52%)
16 (48.48%)
11 (39.29%)
17 (60.71%)
0.912
0.243
2.Gender
Males
Females
9 (37.50%)
15 (62.50%)
22 (59.46%)
15 (40.54%)
2.809
0.120
15 (45.45%)
18 (54.55%)
16 (57.14%)
12 (42.86%)
0.828
0.444
3.Localization
Acral
Upper body
Cervico-cranial
Limbs
3 (12.5%)
7 (29.17%)
9 (37.5%
5 (20.83%)
2 (5.41%)
19 (51.35%)
3 (8.11%)
13 (35.14%)
9.603 *
0.018
3 (9.1%)
11 (33.33%)
7 (21.2%)
12 (36.36%)
2 (7.1%)
15 (53.62%)
5 (17.86%)
6 (21.43%)
2.791
0.443
4.Clark level of
invasion
I
II
III
IV
V

6 (25%)
2 (8.33%)
4(16.67%)
11(45.83%)
1(4.17%)

3 (8.11%)
4 (10.81%)
6(16.22%)
21 (56.76%)
3 (8.11%)

3.584

0.494

7 (21.22%)
1 (3.03%)
3 (9.09%)
19 (57.58%)
3 (9.09%)

2(7.14%)
5 (17.86%)
7 (25.00%)
13 (46.43%)
1 (3.57%)

8.819

0.068
5.Breslow tumor thickness
<1 mm
1–2 mm
2–4 mm
>4 mm

4 (16.67%)
0 (0%)
3 (12.50%)
17 (70.83%)

2 (5.41%)
3 (8.11%)
12 (32.43%)
20 (54.05%)

6.851

0.086

6 (18.18%)
0 (0%)
6 (18.18%)
21 (63.64%)

0 (0%)
3 (10.71%)
9 (32.14%)
16 (57.14%)

9.933 *

0.014
6.Lymphovascular invasion
Present
Absent

10 (41.67%)
14(52.14%)

24 (64.86%)
13(35.14%)

3.175

0.113

16 (48.48%)
17 (51.52%)

18 (64.29%)
10 (35.71%)

1.533

0.216
7.Perineural
invasion
Present
Absent

5 (20.83%)
19 (79.17%)

9 (14.75%)
33 (89.19)

1.163

0.298

5 (15.15%)
28 (84.85%)

4 (14.29%)
24 (85.71%)

0.009

0.926
PD-L1- Programmed cell death ligand-1; TILs-tumor-infiltrating lymphocytes; MTCs-melanoma tumor cells; * p < 0.05 represents statistically significant differences between cases made by Pearson Chi-Square with Cramer’s V test for the association between categorical variables, Mann-Whitney U Test and Kruskal-Wallis H Test for Continuous variables (X2 Score).
Table 2. Association between PD-L1 (+/-) expressions in tumor microenvironment cells and CD4 and CD8 tumor-infiltrating lymphocytes.
Table 2. Association between PD-L1 (+/-) expressions in tumor microenvironment cells and CD4 and CD8 tumor-infiltrating lymphocytes.
Nb.Morphological FeaturesInfiltrating Immune Cells X2
Score		p-ValueMelanoma Tumor CellsX2
Score		p-Value
PD-L1 (-)
n (%)		PD-L1 (+)
n (%)		PD-L1 (-)
n (%)		PD-L1 (+)
n (%)
1.Morphologic type
LMM
SSM
SSM with VGN
NM
2 (8.33%)
7 (29.17%)
6 (25%)
9 (37.50%)
1 (2.70%)
14 (37.84%)
14 (37.84%)
8 (21.62%)

3.305

0.332
3 (9.09%)
8 (24.24%)
13 (39.39%)
9 (27.27%)
0 (0%)
13 (46.43%)
7 (25%)
8 (28.57%)

5.678

0.144
2.TILs
evaluation
Absent
Brisk
Non-brisk

8 (33.33%)
3 (12.50%)
13 (54.17%)

2 (5.41%)
8 (21.62%)
27 (72.97%)

8.383 *

0.020

7 (21.21%)
4 (12.12%)
22 (66.67%)

3 (10.71%)
7 (25%)
18 (64.29%)

2.425

0.287
3.CD8 TIL’s
Negative
<5%
5–50%
>50%
9(37.50%)
1(4.17%)
14(58.33%)
0 (0.00%)
2 (5.41%)
1 (2.70%)
32 (69.57%)
2 (5.41%)

11.238 **

0.003
7 (21.21%)
0(0.00%)
25 (75.76%)
1 (50.00%)
4 (14.29%)
2 (7.14%)
21 (75.00%)
1 (50.00%)

2.775

0.514
4.CD4 TIL’s
Negative
<5%
5–50%
>50
6 (25.00%)
1 (4.17%)
17 (70.83%)
0 (0.00%)
0 (0.00%)
0 (0.00%)
36 (97.30%)
1 (2.70%)

12.614 **

0.001
3 (9.09%)
0 (0.00%)
29 (87.88%)
1 (3.03%)
3 (10.71%)
1 (3.57%)
24 (85.71%)
0 (0.00%)

2.076

0.906
PD-L1- Programmed cell death ligand-1; TILs- tumor-infiltrating lymphocytes; LMM- lentigo maligna melanoma; SSM- Superficial spreading melanoma; SSM-VGN- superficial spreading melanoma with vertical growth nodule; NM- nodular melanoma; * p < 0.05 and ** p ≤ 0.001 represent statistically significant differences between cases made by Pearson Chi-Square with Cramer’s V test for the association between categorical variables, Mann-Whitney U Test and Kruskal-Wallis H Test for Continuous variables (X2 Score).
Table 3. Receiver operating characteristic (ROC) analysis of PD-L1, CD4, and CD8 tumor-infiltrating lymphocyte biomarkers in melanoma patients.
Table 3. Receiver operating characteristic (ROC) analysis of PD-L1, CD4, and CD8 tumor-infiltrating lymphocyte biomarkers in melanoma patients.
Nb.BiomarkersAUC95% CI *p-ValueYouden J
Index		Cut-Off ValueSensitivitySpecificity
1.PD-L1 TILs0.9590.875 to 0.993<0.0010.889>397.3091.67
2.PD-L1 M0.9750.898 to 0.998<0.0010.934>096.4396.97
3.CD4 M0.9500.862 to 0.989<0.0010.743>1584.3190.00
4.CD8 M0.9410.850 to 0.985<0.0010.758>1089.1386.67
UC = area under the curve; ROC = receiver operating characteristic; * Confidence interval; Youden J = sensitivity + specificity – 100; PD-L1- Programmed cell death ligand-1; TILs- tumor-infiltrating lymphocytes; M- melanoma tumor cells.
Table 4. Logistic regression of potential prognostic values of PD-L1, CD4, and CD8 TILs in melanoma tumor microenvironment cells.
Table 4. Logistic regression of potential prognostic values of PD-L1, CD4, and CD8 TILs in melanoma tumor microenvironment cells.
Nb.Clinical VariablesMultivariate Analysis
Melanoma Tumor Microenvironment Cells
Hazard Ratiop-Value95% CI *
1.Lymphovascular invasion3.0490.5280.095−97.914
2.Perineural invasion0.026 *0.0230.001−0.607
3.PD-L12.7000.4550.198−36.683
4.CD4 TILs11.838 *0.0231.386−101.108
5.CD8 TILs7.748 *0.0181.408−42.622
CI∗- Confidence interval; * p < 0.05 represents statistically significant differences between variables made by Cox Logistic & Snell regression (Cox & Snell R2). PDL-1 in MTC-R2 = 0.23, p = 0.43; CD8 TILs in MTC – R2 = 0.49, p < 0.0001; CD4 TILs in MTC - R2 = 0.38, p = 0.002.
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MDPI and ACS Style

Caraban, B.M.; Matei, E.; Cozaru, G.C.; Aşchie, M.; Deacu, M.; Enciu, M.; Bălţătescu, G.I.; Chisoi, A.; Dobrin, N.; Petcu, L.; et al. PD-L1, CD4+, and CD8+ Tumor-Infiltrating Lymphocytes (TILs) Expression Profiles in Melanoma Tumor Microenvironment Cells. J. Pers. Med. 2023, 13, 221. https://doi.org/10.3390/jpm13020221

AMA Style

Caraban BM, Matei E, Cozaru GC, Aşchie M, Deacu M, Enciu M, Bălţătescu GI, Chisoi A, Dobrin N, Petcu L, et al. PD-L1, CD4+, and CD8+ Tumor-Infiltrating Lymphocytes (TILs) Expression Profiles in Melanoma Tumor Microenvironment Cells. Journal of Personalized Medicine. 2023; 13(2):221. https://doi.org/10.3390/jpm13020221

Chicago/Turabian Style

Caraban, Bogdan Marian, Elena Matei, Georgeta Camelia Cozaru, Mariana Aşchie, Mariana Deacu, Manuela Enciu, Gabriela Izabela Bălţătescu, Anca Chisoi, Nicolae Dobrin, Lucian Petcu, and et al. 2023. "PD-L1, CD4+, and CD8+ Tumor-Infiltrating Lymphocytes (TILs) Expression Profiles in Melanoma Tumor Microenvironment Cells" Journal of Personalized Medicine 13, no. 2: 221. https://doi.org/10.3390/jpm13020221

APA Style

Caraban, B. M., Matei, E., Cozaru, G. C., Aşchie, M., Deacu, M., Enciu, M., Bălţătescu, G. I., Chisoi, A., Dobrin, N., Petcu, L., Gheorghe, E., Hangan, L.-T., Roșu, M. C., Orasanu, C. I., & Nicolau, A.-A. (2023). PD-L1, CD4+, and CD8+ Tumor-Infiltrating Lymphocytes (TILs) Expression Profiles in Melanoma Tumor Microenvironment Cells. Journal of Personalized Medicine, 13(2), 221. https://doi.org/10.3390/jpm13020221

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