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Open AccessArticle

Platelet-Rich Plasma Ameliorates Cyclophosphamide-Induced Acute Interstitial Cystitis/Painful Bladder Syndrome in a Rat Model

1
Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan
2
Departments of Medical Research, Urology and Obstetrics and Gynecology, China Medical University Hospital, Taichung 40447, Taiwan
3
Department of Psychology, College of Medical and Health Science, Asia University, Taichung 41354, Taiwan
4
Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan
5
Department of Anesthesiology, China Medical University Hsinchu Hospital, Hsinchu 30272, Taiwan
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Department of Medicinal Botanicals and Health Applications, Da Yeh University, Changhua 51591, Taiwan
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Department of Respiratory Therapy, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
8
Department of Applied Cosmetology, Hungkuang University, Taichung 43302, Taiwan
9
Department of Chinese Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan
10
Department of Neurology, Kuang Tien General Hospital, Taichung 43303, Taiwan
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(6), 381; https://doi.org/10.3390/diagnostics10060381
Received: 1 May 2020 / Revised: 4 June 2020 / Accepted: 4 June 2020 / Published: 8 June 2020
(This article belongs to the Special Issue Animal Models for Studying and Screening Human Diseases)
Background: Interstitial cystitis/painful bladder syndrome (IC/PBS) could be treated to ameliorate urothelial injury. Here, we investigated the efficacy of intravesical instillation with platelet-rich plasma (PRP) and hyaluronic acid for acute IC/PBS. Methods: The effects of PRP and hyaluronic acid on the proliferation of normal human fibroblast cells (HFCs) were assessed. Additionally, thirty virgin female rats were randomized into five groups: group 1, saline-injected control; group 2, cyclophosphamide (CYP) plus intravesical instillation with normal saline; group 3, CYP plus intravesical instillation with hyaluronic acid (1 mg/mL); group 4, CYP plus intravesical instillation with PRP; and group 5, CYP plus intravesical instillation with PRP plus hyaluronic acid. A cystometry and histological assessments were performed. The expression of cell junction-associated protein zonula occludens-2 (ZO-2) and inflammatory cytokine interleukin 6 (IL-6) was also measured. Results: Low dose PRP increased proliferation in HFCs. The acute IC/PBS rats showed significantly lower voiding interval values. Voiding interval values were significantly higher in the CYP plus intravesical instillation with PRP group than in the CYP-induced acute IC/PBS group. Additionally, the expression of ZO-2 was increased and IL-6 was decreased in the CYP plus intravesical instillation with PRP group compared with the CYP-induced acute IC/PBS group. Conclusion: These findings suggest that PRP modulate urothelial repair, which ameliorate the increase in urination frequency in rats treated with CYP. Overall, PRP may confer potential benefits by acting as urothelial repair modulators. View Full-Text
Keywords: platelet-rich plasma; interstitial cystitis; painful bladder syndrome; cyclophosphamide platelet-rich plasma; interstitial cystitis; painful bladder syndrome; cyclophosphamide
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MDPI and ACS Style

Chen, Y.-H.; Man, K.-M.; Chen, W.-C.; Liu, P.-L.; Tsai, K.-S.; Tsai, M.-Y.; Wu, Y.-T.; Chen, H.-Y. Platelet-Rich Plasma Ameliorates Cyclophosphamide-Induced Acute Interstitial Cystitis/Painful Bladder Syndrome in a Rat Model. Diagnostics 2020, 10, 381.

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