Molecular Cloning and Functional Analysis of IrUGT86A1-like Gene in Medicinal Plant Isodon rubescens (Hemsl.) Hara
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Sample Preparation
2.2. Total RNA Extraction and cDNA Reverse Transcription
2.3. Cloning of IrUGT86A1-like Gene
2.4. Bioinformatics Analysis
2.5. Multi-Sequence Alignment and Construction of Phylogenetic Tree
2.6. Fluorescence Quantitative Expression Analysis
2.7. Determination of Oridonin in I. rubescens
2.8. Prokaryotic Expression Analysis
3. Results
3.1. Cloning of IrUGT86A1-like Gene from I. rubescens
3.2. Physicochemical Properties and Localization Analysis of IrUGT86A1-like Protein
3.3. Structural Analysis of IrUGT86A1-like Protein from I. rubescens
3.4. Phylogenetic Analysis of IrUGT86A1-like in I. rubescens
3.5. IrUGT86A1-like Expression and Oridonin Content in Different Tissues of I. rubescens
3.6. IrUGT86A1-Like Expression under Different Abiotic Treatments of I. rubescens
3.7. Prokaryotic Expression Analysis of IrUGT86A1-like Protein in I. rubescens
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lian, C.; Lan, J.; Zhang, B.; Yang, H.; Guo, K.; Li, J.; Chen, S. Molecular Cloning and Functional Analysis of IrUGT86A1-like Gene in Medicinal Plant Isodon rubescens (Hemsl.) Hara. Life 2022, 12, 1334. https://doi.org/10.3390/life12091334
Lian C, Lan J, Zhang B, Yang H, Guo K, Li J, Chen S. Molecular Cloning and Functional Analysis of IrUGT86A1-like Gene in Medicinal Plant Isodon rubescens (Hemsl.) Hara. Life. 2022; 12(9):1334. https://doi.org/10.3390/life12091334
Chicago/Turabian StyleLian, Conglong, Jinxu Lan, Bao Zhang, Hao Yang, Kaihua Guo, Jingjing Li, and Suiqing Chen. 2022. "Molecular Cloning and Functional Analysis of IrUGT86A1-like Gene in Medicinal Plant Isodon rubescens (Hemsl.) Hara" Life 12, no. 9: 1334. https://doi.org/10.3390/life12091334
APA StyleLian, C., Lan, J., Zhang, B., Yang, H., Guo, K., Li, J., & Chen, S. (2022). Molecular Cloning and Functional Analysis of IrUGT86A1-like Gene in Medicinal Plant Isodon rubescens (Hemsl.) Hara. Life, 12(9), 1334. https://doi.org/10.3390/life12091334