Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Cerebroventricular Cannulation for Intracerebroventricular Injection
2.3. Drug Administration
2.4. Rat Model of Ischemic Stroke Was Prepared by MCAO/Reperfusion
2.5. Neurological Deficit Score Was Assessed to Evaluate the Pharmacological Effects of MEL
2.6. The Brain Infarct Volume Was Measured by TTC Staining
2.7. Neuronal Survival Was Detected by Nissl Staining
2.8. Neuron Loss Was Detected by Fluoro-Jade C Staining
2.9. Western Blot
2.10. Immunofluorescence
2.11. Statistical Analysis
3. Results
3.1. MEL Prominently Ameliorated the Autophagy Flux at the Penumbra after Ischemic Stroke
3.2. The MEL-Ameliorated Autophagy Flux Might Be Achieved by Induction of TFEB Nuclear Translocation in Neurons
3.3. Enhancing the Lysosomal Capacity Was the Main Contribution of MEL-Boosted TFEB Nuclear Translocation
3.4. MEL Dramatically Promoted Neuron Survival at the Penumbra
3.5. MEL Dramatically Attenuated Brain Infarct Volume after MCAO/Reperfusion
3.6. MEL Markedly Alleviated the Neurological Deficit and Reduced Animal Mortality after Ischemic Stroke
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wu, Z.; Zhang, Y.; Liu, Y.; Chen, X.; Huang, Z.; Zhao, X.; He, H.; Deng, Y. Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons. Life 2021, 11, 948. https://doi.org/10.3390/life11090948
Wu Z, Zhang Y, Liu Y, Chen X, Huang Z, Zhao X, He H, Deng Y. Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons. Life. 2021; 11(9):948. https://doi.org/10.3390/life11090948
Chicago/Turabian StyleWu, Zhiyuan, Yongjie Zhang, Yuyuan Liu, Xuemei Chen, Zhiwen Huang, Xiaoming Zhao, Hongyun He, and Yihao Deng. 2021. "Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons" Life 11, no. 9: 948. https://doi.org/10.3390/life11090948