Taguchi statistical design, an orthogonal array (OA) method, was used to study the impact of the COD/SO
42− ratio, hydraulic retention time (HRT) and linoleic acid (LA) concentration on sulfate (SO
42−) reduction in an anaerobic sequencing batch reactor using
[...] Read more.
Taguchi statistical design, an orthogonal array (OA) method, was used to study the impact of the COD/SO
42− ratio, hydraulic retention time (HRT) and linoleic acid (LA) concentration on sulfate (SO
42−) reduction in an anaerobic sequencing batch reactor using glucose as the electron donor. Based on the OA, optimum condition for maximum SO
42− reduction was evaluated. Increasing the COD/SO
42− ratio and HRT caused decreasing SO
42− reduction while increased SO
42− reduction was observed with increasing LA concentration (1 g L
−1). In control (not fed LA) cultures, higher SO
42− reduction (87% ± 3%) was observed at a low COD/SO
42− ratio of 0.8. This indicates that increasing SO
42− reduction was observed at increasing SO
42− loading rates. In general, results from this study reveal that limiting the substrate concentration with high SO
42− levels (low COD/SO
42− ratio) favors high SO
42− removal. Surface plots were used to evaluate the significant interactions between the experimental factors. Accuracy of the model was verified using an analysis of residuals. Optimum conditions for maximum SO
42− reduction (97.61%) were observed at a COD/SO
42− ratio of 0.8 (level 1), 12 h HRT (level 1) together with 1000 mg L
−1 LA addition (level 3). In general, the Taguchi OA provided a useful approach for predicting the percent SO
42− reduction in inhibited mixed anaerobic cultures within the factor levels investigated.
Full article