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Article

Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons

1
Interdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edifício do Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal
2
Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
3
Faculty of Sciences, University of Porto, Rua do Campo Alegre 790, 4150-171 Porto, Portugal
*
Author to whom correspondence should be addressed.
Water 2021, 13(1), 66; https://doi.org/10.3390/w13010066
Received: 13 November 2020 / Revised: 22 December 2020 / Accepted: 26 December 2020 / Published: 31 December 2020
Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera Pseudomonas, Rhodococcus and Acinetobacter. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA/P) and bioaugmentation with inoculum pre-grown in acetate (BA/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA/P and BA/A but not in NA. Acetate was the best substrate for consortium growth. BA/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills. View Full-Text
Keywords: autochthonous bacteria; oil spills; bioaugmentation; biostimulation; petroleum; bioremediation; beach sediments autochthonous bacteria; oil spills; bioaugmentation; biostimulation; petroleum; bioremediation; beach sediments
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MDPI and ACS Style

Perdigão, R.; Almeida, C.M.R.; Santos, F.; Carvalho, M.F.; Mucha, A.P. Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons. Water 2021, 13, 66. https://doi.org/10.3390/w13010066

AMA Style

Perdigão R, Almeida CMR, Santos F, Carvalho MF, Mucha AP. Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons. Water. 2021; 13(1):66. https://doi.org/10.3390/w13010066

Chicago/Turabian Style

Perdigão, Rafaela, C. Marisa R. Almeida, Filipa Santos, Maria F. Carvalho, and Ana P. Mucha. 2021. "Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons" Water 13, no. 1: 66. https://doi.org/10.3390/w13010066

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