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Open AccessArticle

Phosphate Deficiency Negatively Affects Early Steps of the Symbiosis between Common Bean and Rhizobia

1
Laboratorio de Genómica Funcional de Leguminosas, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de Mexico, Tlalnepantla 54090, Estado de Mexico, Mexico
2
Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de Mexico, Coyoacan 04510, Ciudad de Mexico, Mexico
3
Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Beadle Center, Lincoln, NE 68503, USA
4
Informatics Institute, University of Missouri, Columbia, MO 65211, USA
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Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA
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Department of Health Management and Informatics, School of Medicine, University of Missouri, Columbia, MO 65211, USA
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Departamento de Biología de Sistemas y Biología Sintética, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de Mexico, Cuernavaca 62210, Morelos, Mexico
8
Division of Plant Sciences, C. S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2018, 9(10), 498; https://doi.org/10.3390/genes9100498
Received: 11 September 2018 / Revised: 5 October 2018 / Accepted: 10 October 2018 / Published: 15 October 2018
(This article belongs to the Section Plant Genetics and Genomics)
Phosphate (Pi) deficiency reduces nodule formation and development in different legume species including common bean. Despite significant progress in the understanding of the genetic responses underlying the adaptation of nodules to Pi deficiency, it is still unclear whether this nutritional deficiency interferes with the molecular dialogue between legumes and rhizobia. If so, what part of the molecular dialogue is impaired? In this study, we provide evidence demonstrating that Pi deficiency negatively affects critical early molecular and physiological responses that are required for a successful symbiosis between common bean and rhizobia. We demonstrated that the infection thread formation and the expression of PvNSP2, PvNIN, and PvFLOT2, which are genes controlling the nodulation process were significantly reduced in Pi-deficient common bean seedlings. In addition, whole-genome transcriptional analysis revealed that the expression of hormones-related genes is compromised in Pi-deficient seedlings inoculated with rhizobia. Moreover, we showed that regardless of the presence or absence of rhizobia, the expression of PvRIC1 and PvRIC2, two genes participating in the autoregulation of nodule numbers, was higher in Pi-deficient seedlings compared to control seedlings. The data presented in this study provides a mechanistic model to better understand how Pi deficiency impacts the early steps of the symbiosis between common bean and rhizobia. View Full-Text
Keywords: phosphorus deficiency; common bean; rhizobia; molecular dialog; symbiosis; gene transcription phosphorus deficiency; common bean; rhizobia; molecular dialog; symbiosis; gene transcription
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Isidra-Arellano, M.C.; Reyero-Saavedra, M.D.R.; Sánchez-Correa, M.D.S.; Pingault, L.; Sen, S.; Joshi, T.; Girard, L.; Castro-Guerrero, N.A.; Mendoza-Cozatl, D.G.; Libault, M.; Valdés-López, O. Phosphate Deficiency Negatively Affects Early Steps of the Symbiosis between Common Bean and Rhizobia. Genes 2018, 9, 498.

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