Next Article in Journal
Comprehensive Genome-Wide Exploration of C2H2 Zinc Finger Family in Grapevine (Vitis vinifera L.): Insights into the Roles in the Pollen Development Regulation
Previous Article in Journal
The Power of Yeast in Modelling Human Nuclear Mutations Associated with Mitochondrial Diseases
 
 
Article

Understanding Rice-Magnaporthe Oryzae Interaction in Resistant and Susceptible Cultivars of Rice under Panicle Blast Infection Using a Time-Course Transcriptome Analysis

1
ICAR-National Institute for Plant Biotechnology, New Delhi 110012, India
2
Jamia Hamdard, Hamdard Nagar, New Delhi 110012, India
3
ICAR-Indian Agricultural Statistics Research Institute, New Delhi 110012, India
4
Indian Council of Agricultural Research, New Delhi 110012, India
*
Author to whom correspondence should be addressed.
Academic Editor: Bing Yang
Genes 2021, 12(2), 301; https://doi.org/10.3390/genes12020301
Received: 18 January 2021 / Revised: 15 February 2021 / Accepted: 16 February 2021 / Published: 20 February 2021
(This article belongs to the Section Plant Genetics and Genomics)
Rice blast is a global threat to food security with up to 50% yield losses. Panicle blast is a more severe form of rice blast and the response of rice plant to leaf and panicle blast is distinct in different genotypes. To understand the specific response of rice in panicle blast, transcriptome analysis of blast resistant cultivar Tetep, and susceptible cultivar HP2216 was carried out using RNA-Seq approach after 48, 72 and 96 h of infection with Magnaporthe oryzae along with mock inoculation. Transcriptome data analysis of infected panicle tissues revealed that 3553 genes differentially expressed in HP2216 and 2491 genes in Tetep, which must be the responsible factor behind the differential disease response. The defense responsive genes are involved mainly in defense pathways namely, hormonal regulation, synthesis of reactive oxygen species, secondary metabolites and cell wall modification. The common differentially expressed genes in both the cultivars were defense responsive transcription factors, NBS-LRR genes, kinases, pathogenesis related genes and peroxidases. In Tetep, cell wall strengthening pathway represented by PMR5, dirigent, tubulin, cell wall proteins, chitinases, and proteases was found to be specifically enriched. Additionally, many novel genes having DOMON, VWF, and PCaP1 domains which are specific to cell membrane were highly expressed only in Tetep post infection, suggesting their role in panicle blast resistance. Thus, our study shows that panicle blast resistance is a complex phenomenon contributed by early defense response through ROS production and detoxification, MAPK and LRR signaling, accumulation of antimicrobial compounds and secondary metabolites, and cell wall strengthening to prevent the entry and spread of the fungi. The present investigation provided valuable candidate genes that can unravel the mechanisms of panicle blast resistance and help in the rice blast breeding program. View Full-Text
Keywords: rice; panicle blast; RNA-Seq; Magnaporthe; cell wall modification; disease resistance rice; panicle blast; RNA-Seq; Magnaporthe; cell wall modification; disease resistance
Show Figures

Figure 1

MDPI and ACS Style

Kumar, V.; Jain, P.; Venkadesan, S.; Karkute, S.G.; Bhati, J.; Abdin, M.Z.; Sevanthi, A.M.; Mishra, D.C.; Chaturvedi, K.K.; Rai, A.; Sharma, T.R.; Solanke, A.U. Understanding Rice-Magnaporthe Oryzae Interaction in Resistant and Susceptible Cultivars of Rice under Panicle Blast Infection Using a Time-Course Transcriptome Analysis. Genes 2021, 12, 301. https://doi.org/10.3390/genes12020301

AMA Style

Kumar V, Jain P, Venkadesan S, Karkute SG, Bhati J, Abdin MZ, Sevanthi AM, Mishra DC, Chaturvedi KK, Rai A, Sharma TR, Solanke AU. Understanding Rice-Magnaporthe Oryzae Interaction in Resistant and Susceptible Cultivars of Rice under Panicle Blast Infection Using a Time-Course Transcriptome Analysis. Genes. 2021; 12(2):301. https://doi.org/10.3390/genes12020301

Chicago/Turabian Style

Kumar, Vishesh, Priyanka Jain, Sureshkumar Venkadesan, Suhas Gorakh Karkute, Jyotika Bhati, Malik Zainul Abdin, Amitha Mithra Sevanthi, Dwijesh Chandra Mishra, Krishna Kumar Chaturvedi, Anil Rai, Tilak Raj Sharma, and Amolkumar U. Solanke. 2021. "Understanding Rice-Magnaporthe Oryzae Interaction in Resistant and Susceptible Cultivars of Rice under Panicle Blast Infection Using a Time-Course Transcriptome Analysis" Genes 12, no. 2: 301. https://doi.org/10.3390/genes12020301

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop