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Article

Methanogenesis and Salt Tolerance Genes of a Novel Halophilic Methanosarcinaceae Metagenome-Assembled Genome from a Former Solar Saltern

1
Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
2
Southern California Coastal Water Research Project, Costa Mesa, CA 92626, USA
3
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Avelino Álvarez-Ordoñez
Genes 2021, 12(10), 1609; https://doi.org/10.3390/genes12101609
Received: 21 September 2021 / Revised: 5 October 2021 / Accepted: 9 October 2021 / Published: 13 October 2021
(This article belongs to the Section Microbial Genetics and Genomics)
Anaerobic archaeal methanogens are key players in the global carbon cycle due to their role in the final stages of organic matter decomposition in anaerobic environments such as wetland sediments. Here we present the first draft metagenome-assembled genome (MAG) sequence of an unclassified Methanosarcinaceae methanogen phylogenetically placed adjacent to the Methanolobus and Methanomethylovorans genera that appears to be a distinct genus and species. The genome is derived from sediments of a hypersaline (97–148 ppt chloride) unrestored industrial saltern that has been observed to be a significant methane source. The source sediment is more saline than previous sources of Methanolobus and Methanomethylovorans. We propose a new genus name, Methanosalis, to house this genome, which we designate with the strain name SBSPR1A. The MAG was binned with CONCOCT and then improved via scaffold extension and reassembly. The genome contains pathways for methylotrophic methanogenesis from trimethylamine and dimethylamine, as well as genes for the synthesis and transport of compatible solutes. Some genes involved in acetoclastic and hydrogenotrophic methanogenesis are present, but those pathways appear incomplete in the genome. The MAG was more abundant in two former industrial salterns than in a nearby reference wetland and a restored wetland, both of which have much lower salinity levels, as well as significantly lower methane emissions than the salterns. View Full-Text
Keywords: methanogenesis; salt tolerance; anaerobic; archaea; phylogenomics methanogenesis; salt tolerance; anaerobic; archaea; phylogenomics
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MDPI and ACS Style

Bueno de Mesquita, C.P.; Zhou, J.; Theroux, S.M.; Tringe, S.G. Methanogenesis and Salt Tolerance Genes of a Novel Halophilic Methanosarcinaceae Metagenome-Assembled Genome from a Former Solar Saltern. Genes 2021, 12, 1609. https://doi.org/10.3390/genes12101609

AMA Style

Bueno de Mesquita CP, Zhou J, Theroux SM, Tringe SG. Methanogenesis and Salt Tolerance Genes of a Novel Halophilic Methanosarcinaceae Metagenome-Assembled Genome from a Former Solar Saltern. Genes. 2021; 12(10):1609. https://doi.org/10.3390/genes12101609

Chicago/Turabian Style

Bueno de Mesquita, Clifton P., Jinglie Zhou, Susanna M. Theroux, and Susannah G. Tringe. 2021. "Methanogenesis and Salt Tolerance Genes of a Novel Halophilic Methanosarcinaceae Metagenome-Assembled Genome from a Former Solar Saltern" Genes 12, no. 10: 1609. https://doi.org/10.3390/genes12101609

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