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Cryopreservation and the Freeze–Thaw Stress Response in Yeast

Department of Biology, High Point University, High Point, NC 27268, USA
Author to whom correspondence should be addressed.
Genes 2020, 11(8), 835;
Received: 7 July 2020 / Revised: 19 July 2020 / Accepted: 20 July 2020 / Published: 22 July 2020
(This article belongs to the Special Issue Genetic Aspects of Yeast: Cell Biology, Ecology and Biotechnology)
The ability of yeast to survive freezing and thawing is most frequently considered in the context of cryopreservation, a practical step in both industrial and research applications of these organisms. However, it also relates to an evolved ability to withstand freeze–thaw stress that is integrated with a larger network of survival responses. These responses vary between different strains and species of yeast according to the environments to which they are adapted, and the basis of this adaptation appears to be both conditioned and genetic in origin. This review article briefly touches upon common yeast cryopreservation methods and describes in detail what is known about the biochemical and genetic determinants of cell viability following freeze–thaw stress. While we focus on the budding yeast Saccharomyces cerevisiae, in which the freeze–thaw stress response is best understood, we also highlight the emerging diversity of yeast freeze–thaw responses as a manifestation of biodiversity among these organisms. View Full-Text
Keywords: yeast; cryopreservation; cryoprotectants; freeze–thaw stress response yeast; cryopreservation; cryoprotectants; freeze–thaw stress response
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MDPI and ACS Style

Cabrera, E.; Welch, L.C.; Robinson, M.R.; Sturgeon, C.M.; Crow, M.M.; Segarra, V.A. Cryopreservation and the Freeze–Thaw Stress Response in Yeast. Genes 2020, 11, 835.

AMA Style

Cabrera E, Welch LC, Robinson MR, Sturgeon CM, Crow MM, Segarra VA. Cryopreservation and the Freeze–Thaw Stress Response in Yeast. Genes. 2020; 11(8):835.

Chicago/Turabian Style

Cabrera, Elizabeth, Laylah C. Welch, Meaghan R. Robinson, Candyce M. Sturgeon, Mackenzie M. Crow, and Verónica A. Segarra 2020. "Cryopreservation and the Freeze–Thaw Stress Response in Yeast" Genes 11, no. 8: 835.

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